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Didier Trono

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Related publications (149)

Page 4 of 8

Activity analysis of housekeeping promoters using self-inactivating lentiviral vector delivery into the mouse retina

Patrick Aebischer, Didier Trono, Nicole Déglon

For most retinal degeneration disorders, no efficient treatment exists to preserve photoreceptors (PRs) and, consequently, to maintain vision. Gene transfer appears to be a promising approach to prevent PR loss. In order to design adequate vectors to targe ...

2003

Genotypic features of lentivirus transgenic mice

Didier Trono, Felix Naef, Denis Duboule, Marc-Olivier Sauvain, Frédéric Schütz

Lentivector-mediated transgenesis is increasingly used, whether for basic studies as an alternative to pronuclear injection of naked DNA or to test candidate gene therapy vectors. In an effort to characterize the genetic features of this approach, we first ...

American Society for Microbiology2008

Lentiviral gene transfer into primary and secondary NOD/SCID repopulating cells

Didier Trono, Romain Zufferey

The ability of lentiviral vectors to transfer genes into human hematopoietic stem cells was studied, using a human immunodeficiency virus 1 (HIV-1)-derived vector expressing the green fluorescence protein (GFP) downstream of the phosphoglycerate kinase (PG ...

American Society of Hematology2000

APOBEC3-independent interferon-induced viral clearance in hepatitis B virus transgenic mice

Didier Trono, Priscilla Turelli, Sonia Verp, Bastien Mangeat, Stéphanie Jost

Interferon (IFN) has been part of the standard treatment of chronic hepatitis B infection for more than 2 decades, yet the mechanism of action of this antiviral remains poorly understood. It was recently observed that members of the human APOBEC family of ...

American Society for Microbiology2008

Transposable elements and their KZFP controllers are drivers of transcriptional innovation in the developing human brain

Didier Trono, Julien Léonard Duc, Evaristo Jose Planet Letschert, Alexandre Coudray, Shaoline Sheppard, Christopher James Playfoot, Sagane Dind

Transposable elements (TEs) account for more than 50% of the human genome and many have been co-opted throughout evolution to provide regulatory functions for gene expression networks. Several lines of evidence suggest that these networks are fine-tuned by ...

COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT2021

Regulation of Episomal Gene Expression by KRAB/KAP1-Mediated Histone Modifications

Patrick Aebischer, Didier Trono, Sonia Verp, Isabelle Barde, Christopher Towne, Vivianne Padrun, Anna Claire Groner

KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studi ...

American Society for Microbiology2009

Large variation in anti-SARS-CoV-2 antibody prevalence among essential workers in Geneva, Switzerland

Didier Trono, Idris Guessous, Francisco Javier Perez Saez, Francesco Pennacchio

Limited data exist on SARS-CoV-2 infection rates across sectors and occupations, hindering our ability to make rational policy, including vaccination prioritization, to protect workers and limit SARS-CoV-2 spread. Here, we present results from our SEROCoV- ...

Nature Research2021

Functional analysis and structural modeling of human APOBEC3G reveal the role of evolutionarily conserved elements in the inhibition of human immunodeficiency virus type 1 infection and Alu transposition

Didier Trono, Priscilla Turelli, Bastien Mangeat

Retroelements are important evolutionary forces but can be deleterious if left uncontrolled. Members of the human APOBEC3 family of cytidine deaminases can inhibit a wide range of endogenous, as well as exogenous, retroelements. These enzymes are structura ...

American Society for Microbiology2009

Novel lentiviral vectors displaying "early acting cytokines" selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cells

Didier Trono

A major limitation of current lentiviral vectors (LVs) is their inability to govern efficient gene transfer into quiescent cells, such as human CD34+ cells that reside into G0 phase of the cell cycle and that are highly enriched in hematopoietic stem cells ...

2005

Transduction of CpG DNA-stimulated primary human B cells with bicistronic lentivectors

Didier Trono

Recently, using HIV-1-derived lentivectors, we obtained efficient transduction of primary human B lymphocytes cocultured with murine EL-4 B5 thymoma cells, but not of isolated B cells activated by CD40 ligation. Coculture with a cell line is problematic fo ...

2005

Scan-based fusion proteins for modulating gene expression and methods of use

Didier Trono, Martina Begnis

The invention provides fusion protein comprising a SCAN domain bearing member of a zinc finger-containing protein, a fragment or variant thereof and a catalytically inactive RNA- guided endonuclease or a DNA adenine methyltransferase. Preferably, the fusio ...

2025

Cell-free method for the quantitative measurement of virus neutralizing antibodies

Didier Trono, Priscilla Turelli

The invention provides an in vitro method for the cell-free quantification of virus neutralizing activity, such as the SARS-CoV-2 neutralizing activity, of biological samples and kit for carrying out the method. The invention also provides a method of scre ...

2022

Microfluidic characterisation reveals broad range of SARS-CoV-2 antibody affinity in human plasma

Didier Trono, Priscilla Turelli

The clinical outcome of SARS-CoV-2 infections, which can range from asymptomatic to lethal, is crucially shaped by the concentration of antiviral antibodies and by their affinity to their targets. However, the affinity of polyclonal antibody responses in p ...

LIFE SCIENCE ALLIANCE LLC2022

Genetic features and genomic targets of human KRAB-zinc finger proteins

Jacques Fellay, Didier Trono, Priscilla Turelli, Christian Axel Wandall Thorball, Julien Léonard Duc, Sandra Eloise Kjeldsen, Evaristo Jose Planet Letschert, Alexandre Coudray, Michaël Imbeault, Cyril David Son-Tuyên Pulver, Romain Forey, Jonas Caspar De Tribolet-Hardy

Krüppel-associated box (KRAB) domain-containing zinc finger proteins (KZFPs) are one of the largest groups of transcription factors encoded by tetrapods, with 378 members in human alone. KZFP genes are often grouped in clusters reflecting amplification by ...

2023

Transposable Elements and Their KRAB-ZFP Controllers Regulate Gene Expression in Adult Tissues

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Julien Léonard Duc, Michaël Imbeault, Marco Cassano, Gabriela Ecco, Annamaria Kauzlaric, Andrea Coluccio

KRAB-containing zinc finger proteins (KRAB-ZFPs) are early embryonic controllers of transposable elements (TEs), which they repress with their cofactor KAP1 through histone and DNA methylation, a process thought to result in irreversible silencing. Using a ...

Cell Press - Elsevier2016

KRAB-zinc finger protein gene expansion in response to active retrotransposons in the murine lineage

Didier Trono, Alberto De Iaco

The Krüppel-associated box zinc finger protein (KRAB-ZFP) family diversified in mammals. The majority of human KRAB-ZFPs bind transposable elements (TEs), however, since most TEs are inactive in humans it is unclear whether KRAB-ZFPs emerged to suppress TE ...

2020

KAP1 is an antiparallel dimer with a functional asymmetry

Didier Trono, Matteo Dal Peraro, Beat Fierz, Davide Demurtas, Louise Catherine Bryan, Maria Josefina Marcaida Lopez, Giulia Fonti, Pierre-Yves Joseph Laurent Helleboid, Alexandra Styliani Kalantzi

KAP1 (KRAB domain-associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In doing so, KAP1 acts both as a platform for macromolecular i ...

Life Science Alliance, LLC2019

Broadly potent anti-SARS-CoV-2 antibody shares 93% of epitope with ACE2 and provides full protection in monkeys

Didier Trono, Henning Paul-Julius Stahlberg, Priscilla Turelli, Florence Pojer, Kelvin Ka Ching Lau, Charlène Mireille Raymonde Raclot, Myriam Isabelle Lamrayah, Yoan Désiré Duhoo, Jérémy Campos, Vanessa Genet

Due to the rapid evolution of SARS-CoV-2 to variants with reduced sensitivity to vaccine-induced humoral immunity and the near complete loss of protective efficacy of licensed therapeutic monoclonal antibodies, we isolated a potent, broad-spectrum neutrali ...

2023

DUX-family transcription factors regulate zygotic genome activation in placental mammals

Didier Trono, Evaristo Jose Planet Letschert, Julien Léonard Duc, Sonia Verp, Andrea Coluccio, Alberto De Iaco

In animal embryos, transcription is mostly silent for several cell divisions, until the release of the first major wave of embryonic transcripts through so-called zygotic genome activation (ZGA). Maternally provided ZGA-triggering factors have been identif ...

2017

Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Julien Léonard Duc, Gabriela Ecco, Andrea Coluccio

BACKGROUND: The KZFP/KAP1 (KRAB zinc finger proteins/KRAB-associated protein 1) system plays a central role in repressing transposable elements (TEs) and maintaining parent-of-origin DNA methylation at imprinting control regions (ICRs) during the wave of g ...

2018