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Giovanni Dietler

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Publications associées (156)

Differential peptide binding to CD40 evokes counteractive responses (vol 73, pg 465, 2012)

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Bhaskar Saha

Elsevier2012

Size-dependent hydrogen uptake behavior of Pd nanoparticles revealed by photonic crystal surface waves

Giovanni Dietler, Serguei Sekatski

We present an optical method of study of nanoparticle properties using photonic crystal surface waves. Palladium nanoparticles were deposited on a surface of a one-dimensional photonic crystal, which supports the propagation of p-polarized optical surface ...

American Institute of Physics2012

FM-AFM constant height imaging and force curves: high resolution study of DNA-tip interactions

Giovanni Dietler, Dusan Vobornik, Susana del Carmen Tobenas Borron, Livan Bladimir Alonso Sarduy, Andrea Cerreta

Interaction of the atomic force microscopy (AFM) tip with the sample can be invasive for soft samples. Frequency Modulation (FM) AFM is gentler because it allows scanning in the non-contact regime where only attractive forces exist between the tip and the ...

Wiley-Blackwell2012

DNA knots and DNA supercoiling

Giovanni Dietler, Andrzej Stasiak, Guillaume Witz

2011

Tightening of DNA knots by supercoiling facilitates their unknotting by type II DNA topoisomerases

Giovanni Dietler, Andrzej Stasiak, Guillaume Witz

Using numerical simulations, we compare properties of knotted DNA molecules that are either torsionally relaxed or supercoiled. We observe that DNA supercoiling tightens knotted portions of DNA molecules and accentuates the difference in curvature between ...

2011

Human topoisomerase II-DNA interaction study by using atomic force microscopy

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy

AFM; DNA topology; Human topoisomerase II; Binding geometry; Time-lapse imaging; Protein–DNA interaction ...

Elsevier2011

Nonkinetic Modeling of the Mechanical Unfolding of Multimodular Proteins: Theory and Experiments

Giovanni Dietler, Serguei Sekatski, Fabrizio Benedetti

We introduce and discuss a novel approach called back-calculation for analyzing force spectroscopy experiments on multimodular proteins. The relationship between the histograms of the unfolding forces for different peaks, corresponding to a different numbe ...

Elsevier2011

Conformation of Ring Polymers in 2D Constrained Environments

Giovanni Dietler, Guillaume Witz, Kristian Rechendorff

The combination of ring closure and spatial constraints has a fundamental effect on the statistics of semiflexible polymers such as DNA. However, studies of the interplay between circularity and constraints are scarce and single-molecule experimental data ...

American Physical Society2011

Ultrasharp carbon whisker optical fiber probes for scanning near-field optical microscopy

Giovanni Dietler, Mounir Driss Mensi, Serguei Sekatski, Andrey Mikhaylov

We report the growth of ultrasharp carbon whiskers onto apertured near-field optical glass fiber probes. The ultrasharp carbon whiskers are produced by the electron-assisted dissociation of residual oil vapors present in the vacuum chamber during the elect ...

SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA2010

Supercoiling Induces Denaturation Bubbles in Circular DNA

Giovanni Dietler

We present a theoretical framework for the thermodynamic properties of supercoiling-induced denaturation bubbles in circular double-stranded DNA molecules. We explore how DNA supercoiling, ambient salt concentration, and sequence heterogeneity impact on th ...

American Physical Society2010

Understanding amyloid aggregation by statistical analysis of atomic force microscopy images

Raffaele Mezzenga, Giovanni Dietler, Paolo De Los Rios, Jérôme Flakowski

The aggregation of proteins is central to many aspects of daily life, including food processing, blood coagulation, eye cataract formation disease and prion-related neurodegenerative infections[1–5]. However, the physical mechanisms responsible for amyloid ...

Nature Publishing Group2010

Force spectroscopy of barnase-barstar single molecule interaction

Giovanni Dietler, Mélanie Favre, Serguei Sekatski, Dmitry Klinov, Andrey Mikhaylov

Results of the single molecule force spectroscopy study of specific interactions between ribonuclease barnase and its inhibitor barstar are presented. Experimental data obtained for the force loading rate ranging 2-70 nN/s are well approximated by a single ...

Wiley-Blackwell2010

Characterization of glycoprotein E C-End of West Nile virus and evaluation of its interaction force with alpha V beta 3 integrin as putative cellular receptor

Giovanni Dietler, Serguei Sekatski

Recombinant polypeptide containing the 260-466 amino acid sequence of West Nile virus (WNV) strain LEIV-Vlg99-27889-human glycoprotein E (gpE, E260-466) was constructed. Immunochemical similarity between the E260-466 and gpE of WNV was proven by enzyme imm ...

2010

Microcontroller-driven fluid-injection system for atomic force microscopy

Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy

We present a programmable microcontroller-driven injection system for the exchange of imaging medium during atomic force microscopy. Using this low-noise system, high-resolution imaging can be performed during this process of injection without disturbance. ...

American Institute of Physics2010

Excluded Volume Effects on Semiflexible Ring Polymers

Giovanni Dietler, Guillaume Witz

Two-dimensional semiflexible polymer rings are studied both by imaging circular DNA adsorbed on a mica surface and by Monte Carlo simulations of phantom polymers as well as of polymers with finite thickness. Comparison of size and shape of the different mo ...

American Chemical Society (ACS)2010

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Giovanni Dietler, Mounir Driss Mensi, Serguei Sekatski, Kanat Dukenbayev

A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor?ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. T ...

2010

Near-field scanning optical microscopy using polymethylmethacrylate optical fiber probes

Giovanni Dietler, Mounir Driss Mensi, Serguei Sekatski, Kanat Dukenbayev

We report the first use of polymethylmethacrylate (PMMA) optical fiber-made probes for scanning near-field optical microscopy (SNOM). The sharp tips were prepared by chemical etching of the fibers in ethyl acetate, and the probes were prepared by proper gl ...

Elsevier2010

Stable -Synuclein Oligomers Strongly Inhibit Chaperone Activity of the Hsp70 System by Weak Interactions with J-domain Co-chaperones

Giovanni Dietler, Mounir Driss Mensi, Hilal Lashuel, Pierre Goloubinoff

alpha-Synuclein aggregation and accumulation in Lewy bodies are implicated in progressive loss of dopaminergic neurons in Parkinson disease and related disorders. In neurons, the Hsp70s and their Hsp40-like J-domain co-chaperones are the only known compone ...

2010

Intracellular nanomanipulation by a photonic-force microscope with real-time acquisition of a 3D stiffness matrix

László Forró, Gérard Gremaud, Andrzej Kulik, Philippe Thévenaz, Michaël Unser, Giovanni Dietler, Stefania Puttini, Sylvia Jeney, Elena Bertseva, Amardeep Singh

A traditional photonic-force microscope (PFM) results in huge sets of data, which requires tedious numerical analysis. In this paper, we propose instead an analog signal processor to attain real-time capabilities while retaining the richness of the traditi ...

Institute of Physics2009

Stiffness Tomography by Atomic Force Microscopy

Charles Roduit, Giovanni Dietler, Sandor Kasas, Serguei Sekatski

The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a namo-indenter to gather information about the mechanical properties of the sample. In thi ...

2009

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