Recherche

Afficher tous les résultats pour

Accueil

Personne

Florian Maria Wurm

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (117)

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, Martin Jordan, Martin Bertschinger, David Hacker, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, David Hacker, Nicolas Jaccard

We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugatio ...

2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells

Florian Maria Wurm, Frédéric Grosjean, Martin Bertschinger, David Hacker

The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter g ...

2006

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Florian Maria Wurm, Madiha Derouazi, Martin Bertschinger, David Hacker

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification sys ...

2006

Polyethylenimine-based quality control assay for plasmid DNA

Florian Maria Wurm, Martin Jordan, Martin Bertschinger, David Hacker, Gaurav Backliwal

2006

Orbital shaker technology for the cultivation of mammalian cells in suspension

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Natalie Muller, David Hacker

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice

Florian Maria Wurm

BACKGROUND: Recent advances in functional genomics, gene therapy, tissue engineering, drug discovery and biopharmaceuticals production have been fostered by precise small-molecule-mediated fine-tuning of desired transgenes. METHODS: Capitalizing on well-ev ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Philippe Pierre Girard, Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Low-temperature pausing of cultivated mammalian cells

Lisa Hunt, Florian Maria Wurm, Martin Jordan, Frédéric Grosjean, David Hacker

There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a v ...

2005

Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?

Florian Maria Wurm, Martin Jordan, David Hacker

2005

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker

The Gam1 protein of the avian CELO adenovirus activates transcription through inhibition of histone deacetylase 1 (HDAC1). We investigated the effect of Gam1 on both transient and stable transgene expression in Chinese hamster ovary (CHO) cells, one of the ...

2005

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells

Florian Maria Wurm, Lucia Baldi Unser, Martin Bertschinger, David Hacker

Human embryonic kidney 293 (HEK293) cells, a widely used host for large-scale transient expression of recombinant proteins, are transformed with the adenovirus E1A and E1B genes. Because the E1A proteins function as transcriptional activators or repressors ...

2004

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Jason Wright, Lucia Baldi Unser, Frédéric Grosjean, Natalie Muller, David Hacker

Springer2002

Transient expression of a soluble and secreted form of heterodimeric T-Cell receptor in Hek-293

Florian Maria Wurm, Martin Jordan

Kluwer Academic1998

Page 6 sur 6

Florian Maria Wurm

Graph Chatbot

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Polyethylenimine-based quality control assay for plasmid DNA

Orbital shaker technology for the cultivation of mammalian cells in suspension

Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Low-temperature pausing of cultivated mammalian cells

Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?

The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells

Pausing of mammalian cells by cold exposure, limits and opportunities

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Transient expression of a soluble and secreted form of heterodimeric T-Cell receptor in Hek-293