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Florian Maria Wurm

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Publications associées (117)

Suspension-adapted Chinese hamster ovary-derived cells expressing green fluorescent protein as a screening tool for biomaterials

Florian Maria Wurm

Synthetic biomaterials play an important role in regenerative medicine. To be effective they must support cell attachment and proliferation in addition to being non-toxic and non-immunogenic. We used a suspension-adapted Chinese hamster ovary-derived cell ...

Springer Netherlands2009

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV f ...

2009

The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells

Florian Maria Wurm, Martin Bertschinger, David Hacker, Jean Cevey

The kinetics of polyethylenimine (PEI)-mediated gene transfer at early times after transfection of Chinese hamster ovary (CHO) cell in suspension were investigated using a novel in vitro assay. Addition of an excess of competitor DNA to the culture medium ...

Humana Press Inc, Springer2008

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Florian Maria Wurm, Madiha Derouazi, David Hacker, Nicolas Jaccard, Sébastien Chenuet

Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (G ...

2008

Compressed collagen-PLA hybrids as scaffold for human bladder cells

Florian Maria Wurm, Jeffrey Alan Hubbell, Peter Frey

2008

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet

Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient ge ...

2008

Recombinant human collagen as a bioactive element in scaffolds for tissue engineering

Florian Maria Wurm

2008

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Recombinant therapeutic proteins produced in mammalian cells represent a major class of biopharmaceuticals. In recent years, their demand has increased dramatically and is now driving the development of a variety of improvements to maximize their expressio ...

Elsevier2008

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, Sophie Bouchet, David Hacker, Stéphanie Tissot, Jean Cevey, Sarah Wulhfard

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Sustained activation and tumor targeting of NKT cells using a CD1d-anti-HER2-scFv fusion protein induce antitumor effects in mice

Florian Maria Wurm, Lucia Baldi Unser

Invariant NKT (iNKT) cells are potent activators of DCs, NK cells, and T cells, and their antitumor activity has been well demonstrated. A single injection of the high-affinity CD1d ligand α-galactosylceramide (αGalCer) leads to short-lived iNKT cell activ ...

2008

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler

Adeno-associated virus (AAV)-based vectors belong to the most promising gene transfer vectors in clinical studies. To provide vector for late-stage clinical trials as well as for a potential commercial phase, a scalable, cGMP-compliant process is required. ...

Springer, Springer Netherlands2007

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Pierre-André Besse, Radivoje Popovic, Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Franck Vincent, Klaus Ehrmann

NMR-microprobes based on solenoids and Helmholtz coils have been microfabricated and NMR-spectra of mammalian cells have successfully been taken. The microfabrication technology developed for these probes consists of three electroplated copper levels for l ...

2007

Recombinant protein production by large-scale transient gene expression in mammalian cells : state of the art and future perspectives

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gen ...

Kluwer Academic Publishers, Springer2007

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Florian Maria Wurm, Mathieu Benjamin Stettler, David Hacker, Xiaowei Zhang

Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese h ...

American Chemical Society2007

NMR spectroscopy and perfusion of mammalian cells using surface microprobes

Pierre-André Besse, Radivoje Popovic, Florian Maria Wurm, Mathieu Benjamin Stettler, Jürgen Brugger, Klaus Ehrmann, Kristopher Pataky

NMR spectra of mammalian cells are taken using surface microprobes that are based on microfabricated planar coils. The surface microprobe resembles a miniaturized Petri dish commonly used in biological research. The diameter of the planar coils is 1 mm. Ch ...

2007

Manufacturing of Biopharmaceuticals and Implications for Biosimilars

Florian Maria Wurm

Karger2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Natalie Muller, David Hacker, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI

Florian Maria Wurm, Gaurav Backliwal

2007

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Madiha Derouazi

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

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