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Matthias Lütolf

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Publications associées (180)

Efficient high-thoughput microfluidic trapping of single cells

Matthias Lütolf, Philippe Renaud, Ana Valero

2009

Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell-Based Assays

Matthias Lütolf, Steffen Cosson, Stefan Kobel

versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to gener ...

2009

Biomimetic PEG hydrogels crosslinked with minimal plasmin-sensitive tri-amino acid peptides

Matthias Lütolf, Jeffrey Alan Hubbell, Martin Ehrbar, Simone Rizzi

Semi-synthetic, proteolytically degradable polymer hydrogels have proven effective as scaffolds to augment bone and skin regeneration in animals. However, high costs due to expensive peptide building blocks pose a significant hurdle towards broad clinical ...

Wiley-Blackwell2009

Perturbation of single hematopoietic stem cell fates in artificial niches

Matthias Lütolf

Hematopoietic stem cells (HSCs) are capable of extensive self-renewal in vivo and are successfully employed clinically to treat hematopoietic malignancies, yet are in limited supply as in culture this self-renewal capacity is lost. Using an approach at the ...

2009

Artificial stem cell niches

Matthias Lütolf

Stem cells are characterized by their dual ability to reproduce themselves (self-renew) and specialize (differentiate), yielding essentially unlimited numbers of daughter cells that can maintain and regenerate tissues. In contrast to their embryonic counte ...

Wiley2009

Tailoring hydrogel degradation and drug release via neighboring amino acid-controlled ester hydrolysis

Matthias Lütolf, Jeffrey Alan Hubbell, Yun Suk Jo, Jay Gantz

We present a versatile scheme to rationally modulate the hydrolysis rate of ester bonds in hydrophilic polymer networks via adjacent charged amino acids. As soluble model systems, two cysteine-bearing oligopeptides containing either positively charged argi ...

2009

Micropatterning of Hydrogels by Soft Embossing

Matthias Lütolf, Thierry Laroche, Monika Limacher, Stefan Kobel, Samy Gobaa

Conventional in situ hydrogel micropatterning techniques work successfully for relatively stiff hydrogels, but they often result in locally damaged surfaces upon demolding in the case of soft and fragile polymer networks formed at low precursor concentrati ...

American Chemical Society2009

Integration column: microwell arrays for mammalian cell culture

Matthias Lütolf

Microwell arrays have emerged as robust and versatile alternatives to conventional mammalian cell culture substrates. Using standard microfabrication processes, biomaterials surfaces can be topographically patterned to comprise high-density arrays of micro ...

2009

Integration column: Artificial ECM: expanding the cell biology toolbox in 3D

Matthias Lütolf

Many crucial cellular processes in our tissues are governed by complex, spatio-temporally regulated interactions between cells and their extracellular matrix (ECM). These interactions can be studied using well-established 3D in vitro model systems such as ...

2009

Biomaterials: Spotlight on hydrogels

Matthias Lütolf

By using light to control the degradation of hydrogel components in space and time, researchers have generated a tool to help them reconstruct functional biological tissues in a culture dish. ...

2009

Designing materials to direct stem-cell fate

Matthias Lütolf

Proper tissue function and regeneration rely on robust spatial and temporal control of biophysical and biochemical microenvironmental cues through mechanisms that remain poorly understood. Biomaterials are rapidly being developed to display and deliver ste ...

2009

Injectable biomimetic matrices to guide impaired wound healing

Matthias Lütolf, Jeffrey Alan Hubbell, Martin Ehrbar, Simone Rizzi, Yushu Joy Xie

2008

Synthetic fibrin-like matrix for bone tissue engineering

Matthias Lütolf, Jeffrey Alan Hubbell, Martin Ehrbar, Simone Rizzi

2008

Part II: Fibroblasts preferentially migrate in the direction of principal strain

Matthias Lütolf, Jeffrey Alan Hubbell

A growing body of evidence suggests that the sensory information from the cytoskeleton and integrins may be responsible for guiding migration during mechano- and haptotaxis. However, the dual function of these subcellular structures as mechano-sensors and ...

Springer-Verlag2008

Enhancing the Reliability and Throughput of Neurosphere Culture on Hydrogel Microwell Arrays

Matthias Lütolf, Monika Limacher, Stefan Kobel

The neurosphere assay is the standard retrospective assay to test the self-renewal capability and multipotency of neural stem cells (NSC) in vitro. However, it has recently become clear that not all neurospheres are derived from a NSC and that on conventio ...

2008

Microfluidics-generated immobilized biomolecule gradients on hydrogels

Matthias Lütolf, Steffen Cosson, Stefan Kobel

Extra-cellular matrix (ECM) tethered morphogen gradients play an important role in tissue development and regeneration. There is currently only a limited set of model systems which can mimic these gradients in an artificial ECM-like environment. We have de ...

The Chemical and Biological Microsystems Society2008

Artificial extracellular matrices for bone tissue engineering

Matthias Lütolf, Jeffrey Alan Hubbell, Martin Ehrbar, Simone Rizzi

2008

Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering

Matthias Lütolf, Jeffrey Alan Hubbell

A review. New generations of synthetic biomaterials are being developed at a rapid pace for use as three-dimensional extracellular microenvironments to mimic the regulatory characteristics of natural extracellular matrixes (ECMs) and ECM-bound growth facto ...

2005

Molecularly engineered PEG hydrogels: A novel model system for proteolytically mediated cell migration

Matthias Lütolf, Jeffrey Alan Hubbell

Model systems mimicking the extracellular matrix (ECM) have greatly helped in quantifying cell migration in three dimensions and elucidated the mol. determinants of cellular motility in morphogenesis, regeneration, and disease progression. Here the authors ...

2005

The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides

Matthias Lütolf, Jeffrey Alan Hubbell

The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse vide ...

2005

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