Lipid exchange and signaling at ER–Golgi contact sites

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STARD4 regulates cholesterol homeostasis by transferring cholesterol between the plasma membrane and endoplasmic reticulum. The STARD4 structure features a helix-grip fold surrounding a large hydrophobic cavity holding the sterol. Its access is controlled ...

The application of computational biology in drug development for membrane protein targets has experienced a boost from recent developments in deep learning-driven structure prediction, increased speed and resolution of structure elucidation, machine learni ...

Endoplasmic reticulum (ER) retention of misfolded glycoproteins is mediated by the ER-localized eukaryotic glycoprotein secretion checkpoint, UDP-glucose glycoprotein glucosyl-transferase (UGGT). The enzyme recognizes a misfolded glycoprotein and flags it ...

Macrocycles offer an attractive format for drug development due to their good binding properties and potential to cross cell membranes. To efficiently identify macrocyclic ligands for new targets, methods for the synthesis and screening of large combinator ...

Post-translational modifications (PTMs) play a pivotal role in regulating protein structure, interaction, and function. Aberrant PTM patterns are associated with diseases. Moreover, individual PTMs have a complex interaction with each other, known as PTM c ...

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Hydrated lipid bilayer membranes are crucial components of cells and organelles, serving as the outer boundary that separates the cellular components from the extracellular environment. Lipid membranes regulate their structures and functions by dynamically ...

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Sphingolipids are a structurally diverse class of lipids predominantly found in the plasma membrane of eukaryotic cells. These lipids can laterally segregate with other rigid lipids and cholesterol into liquid-ordered domains that act as organizing centers ...

A key player in the formation of endoplasmic reticulum sheets is CLIMP-63, but mechanistic details remained elusive. Here authors combined cellular experiments and mathematical modelling to show that S-acylation of CLIMP-63 regulates its function by mediat ...

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Lipid exchange and signaling at ER–Golgi contact sites

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S-acylation and lipid exchange at the ER-Golgi membrane contact sites regulate pathogen entry in human cells

Membrane specificity of the human cholesterol transfer protein STARD4

Computational drug development for membrane protein targets

Rescue of secretion of rare-disease-associated misfolded mutant glycoproteins in UGGT1 knock-out mammalian cells

Large Libraries of Structurally Diverse Macrocycles Suitable for Membrane Permeation

Investigating the intra-molecular and inter-molecular effects of post-translational modifications on intrinsically disordered protein regions and structured protein regions

HaloTag-Based Tools for Live-Cell Imaging and Elucidation of Organellar Redox Biology

Advancements in Nanomechanical Characterization and Biomolecular Imaging with Atomic force Microscopy

From Surfactants to 3D Printing: How Ion-Chelator Pair Interactions Affect the Mechanical Properties of Bioinspired Materials

Simultaneous membrane and RNA binding by Tick-Borne Encephalitis Virus capsid protein

Effects of thermal, mechanical, and electrostatic perturbations on lipid membrane hydration investigated by second harmonic imaging

Simultaneous Electrophysiology and Imaging Reveal Changes in Lipid Membrane Thickness and Tension upon Uptake of Amphiphilic Gold Nanoparticles

Interplay of the mechanical and structural properties of DNA nanostructures determines their electrostatic interactions with lipid membranes

Cholesterol-Containing Liposomes Decorated With Au Nanoparticles as Minimal Tunable Fusion Machinery

Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains

Dynamics of CLIMP-63 S-acylation control ER morphology

Liposomes and Lipid Droplets Display a Reversal of Charge-Induced Hydration Asymmetry

Quantifying single-cell diacylglycerol signaling kinetics after uncaging

Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase

The Bioaccumulation of Ionizable Organic Compounds in Fish Cell Lines