Deep neural network automated segmentation of cellular structures in volume electron microscopy

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Brain organoids enable the mechanistic study of human brain development and provide opportunities to explore self-organization in unconstrained developmental systems1, 2–3. Here we establish long-term, live light-sheet microscopy on unguided brain organoid ...

Single-cell impedance flow cytometry (IFC) is a rapid, label-free diagnostic capable of resolving the dielectric properties of individual cells. Here, single-cell IFC is employed to investigate the effects of plasma-activated water (PAW) on E. coli. This d ...

Correlative light and electron microscopy (CLEM) combines light microscopy, for identifying a target via genetic labels, dyes, antibodies and morphological features, with electron microscopy, for analyzing high-resolution subcellular ultrastructures. Here, ...

An equimolar NiCoFeCrGa high entropy alloy having dual-phase composition was studied, where the constituent phases exhibit distinct mechanical properties. Micropillars with various diameters were created from two differently heat treated samples, then they ...

This dataset contains some images (TIFF image data) of brain juvenile rats Wistar Han (P14) scanned immunostained slides by using an automated slide scanner (Olympus, VS120-L100, GER) equipped with a UPLSAPO 20x/0.75 air objective (Olympus, GER) and a Pi ...

Accessing the low-energy non-equilibrium dynamics of materials and their polaritons with simultaneous high spatial and temporal resolution has been a bold frontier of electron microscopy in recent years. One of the main challenges lies in the ability to re ...

Many eukaryotes form multinucleated cells during their devel-opment. Some cells persist as such during their lifetime, others choose to cleave each nucleus individually using a specialized cytokinetic process known as cellularization. What is cellula-rizat ...

Current methods have limited ability in directly quantifying the extent of glutathionylation on specific proteincysteines. In this issue of Cell Chemical Biology, Ahn et al.1 report G-ICAT (glutathione-based isotope-coded affinity tag), aimed at addressing ...

Simple Summary The cytoplasm of a living cell is a crowded place, containing hundreds of types of protein and other macromolecules. Cells reliably and continually perform thousands of biochemical reactions to maintain their health. Biomolecular condensates ...

The Bio Image and Signal Processing (BISP) Technical Committee (TC) of the IEEE Signal Processing Society (SPS) promotes activities within the broad technical field of biomedical image and signal processing. Areas of interest include medical and biological ...

With the development of modern medical techniques, the application of orthopedic implants has been continually increasing in recent years. Passive materials, such as Ti and its alloys as well as CoCrMo alloys, are widely used in artificial joints due to th ...

Cultured autologous human articular chondrocyte (HAC) implantation has been extensively investigated for safe and effective promotion of structural and functional restoration of knee cartilage lesions. HAC-based cytotherapeutic products for clinical use mu ...

Perpendicular shape anisotropy (PSA) and double magnetic tunnel junctions (DMTJs) offer practical solutions to downscale spin-transfer-torque Magnetic Random-Access Memory (STT-MRAM) beyond 20 nm technology nodes, while retaining their thermal stability an ...

Microscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ra ...

This work reports experimental results of the quantitative determination of oxygen and band gap measurement in the TiNx electrodes in planar TiNx top/La:HfO2/TiNx bottom MIM stacks obtained by plasma enhanced atomic layer deposition on SiO2. Methodological ...

Chemical reactions and phase transformations in nanometer-size objects may happen at very short timescales, so conventional characterization tools are not applicable. A new technique combining high spatial and temporal resolution is ultrafast (or dynamic) ...

In lymphopenic environments, secondary lymphoid organs regulate the size of B and T cell compartments by supporting the homeostatic proliferation of mature lymphocytes. The molecular mechanisms underlying these responses and their functional consequences r ...

Heteroatom doping engineering is deemed to be an adoptable strategy to boost the potassium (K) storage performance of carbonaceous materials. The inevitable issue for this strategy lies in the huge volume expansion originated from the large radius of K+. I ...

Atomic force microscopy (AFM) is the only technique that allows label-free imaging of nanoscale biomolecular dynamics, playing a crucial role in solving biological questions that cannot be addressed by other major bioimaging tools (fluorescence or electron ...

ER-phagy, literally endoplasmic reticulum (ER)-eating, defines the constitutive or regulated clearance of ER portions within metazoan endolysosomes or yeast and plant vacuoles. The advent of electron microscopy led to the first observations of ER-phagy ove ...

Deep neural network automated segmentation of cellular structures in volume electron microscopy

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Morphodynamics of human early brain organoid development

Investigating the effects of plasma-activated water on E. coli with the novel application of single-cell impedance flow cytometry

Correlative light and electron microscopy for human brain and other biological models

Micromechanics reveal strain rate dependent transition between dislocation mechanisms in a dual phase high entropy alloy

Nissl_4, Raw images for Machine learning for histological annotation and quantification of cortical layers

Coherently amplified ultrafast imaging using a free-electron interferometer

Cellularization across eukaryotes: Conserved mechanisms and novel strategies

Tag, you're it: G-ICAT identifies p120-cysteine glutathionylation triggering E-cadherin destabilization

Macromolecular Crowding Is Surprisingly Unable to Deform the Structure of a Model Biomolecular Condensate

From Nano to Macro An overview of the IEEE Bio Image and Signal Processing Technical Committee

In-vivo investigation of electrochemical behavior of Ti and CoCrMo alloy in human synovial fluids

Retrospective Analysis of Autologous Chondrocyte-Based Cytotherapy Production for Clinical Use: GMP Process-Based Manufacturing Optimization in a Swiss University Hospital

Off-axis electron holography for the direct visualization of perpendicular shape anisotropy in nanoscale 3D magnetic random-access-memory devices

The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution

Structure, Oxygen Content and Electric Properties of Titanium Nitride Electrodes in TiNx/La:HfO2/TiNx Stacks Grown by PEALD on SiO2/Si

Analytics at the nanometer and nanosecond scales by short electron pulses in an electron microscope

Stromal Notch ligands foster lymphopenia-driven functional plasticity and homeostatic proliferation of naive B cells

Dual-function engineering to construct ultra-stable anodes for potassium-ion hybrid capacitors: N, O-doped porous carbon spheres

Visualizing intracellular nanostructures of living cells by nanoendoscopy-AFM

ER-phagy responses in yeast, plants, and mammalian cells and their crosstalk with UPR and ERAD