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Suliana Manley

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Related publications (87)

Nanoscale spatial organization of the HoxD gene cluster in distinct transcriptional states

Denis Duboule, Suliana Manley, Aleksandr Benke, Pierre Raymond Fabre

Chromatin condensation plays an important role in the regulation of gene expression. Recently, it was shown that the transcriptional activation of Hoxd genes during vertebrate digit development involves modifications in 3D interactions within and around th ...

2015

Quantitative evaluation of software packages for single-molecule localization microscopy

Michaël Unser, Daniel Sage, Suliana Manley, Hagay Kirshner, Thomas Pengo

The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution ...

Nature Publishing Group2015

Visualizing the HoxD Gene Cluster at the Nanoscale Level

Denis Duboule, Suliana Manley, Aleksandr Benke, Pierre Raymond Fabre

Transcription of HoxD cluster genes in limbs is coordinated by two topologically associating domains (TADs), neighboring the cluster and containing various enhancers. Here, we use a combination of microscopy approaches and chromosome conformation capture t ...

2015

PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy

Suliana Manley, Thomas Pengo, Seamus John Holden

During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful re ...

Oxford University Press2015

Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Suliana Manley, Seamus John Holden, Lina Carlini, Kyle Michael Douglass

Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, whi ...

Public Library of Science2015

Fast live cell imaging at nanometer scale using annihilating filter based low rank Hankel matrix approach

Michaël Unser, Suliana Manley, Lina Carlini

Localization microscopy such as STORM/PALM can achieve a nanometer scale spatial resolution by iteratively localizing fluorescence molecules. It was shown that imaging of densely activated molecules can accelerate temporal resolution which was considered a ...

Spie-Int Soc Optical Engineering2015

3D high-density localization microscopy using hybrid astigmatic/biplane imaging and sparse image reconstruction

Michaël Unser, Suliana Manley, Seamus John Holden, Lina Carlini

Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs ...

Optical Soc Amer2014

High throughput 3D Palm Imaging Elucidates Mechanisms of Bacterial Cell Division

Suliana Manley, Thomas Pengo, Seamus John Holden

Cell Press2014

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Romain Wyss, Pierre Gönczy, Debora Keller, Suliana Manley

SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 ...

Rockefeller University Press2014

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Luc Reymond, Grazvydas Lukinavicius, Suliana Manley, Aleksandr Benke, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

FALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data

Michaël Unser, Cédric René Jean Vonesch, Suliana Manley, Hagay Kirshner, Seamus John Holden, Lina Carlini

Super resolution microscopy such as STORM and (F) PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow te ...

Nature Publishing Group2014

From "There's Plenty of Room at the Bottom" to Seeing What is Actually There

Suliana Manley

Wiley-V C H Verlag Gmbh2014

A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins

Romain Wyss, Julia Gunzenhäuser, Suliana Manley

The assembly process of the human immunodeficiency virus 1 (HIV-1) is driven by the viral polyprotein Gag. Fluorescence imaging of Gag protein fusions is widely performed and has revealed important information on viral assembly. Gag fusion proteins are com ...

Public Library Science2014

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

Suliana Manley, Thomas Pengo, Seamus John Holden, Karin Lederballe Meibom

We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscal ...

National Academy of Sciences2014

Live Intracellular Super-Resolution Imaging Using Site-Specific Stains

Suliana Manley, Lina Carlini

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological feature ...

American Chemical Society2013

Simple buffers for 3D STORM microscopy

Pierre Gönczy, Debora Keller, Suliana Manley, Vinoth Edal Joseph Sundar Rajan

3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging: making dye molecu ...

Optical Soc Amer2013

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Pierre Gönczy, Debora Keller, Suliana Manley

Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolution ...

Public Library of Science2013

Multicolor Single Molecule Tracking of Stochastically Active Synthetic Dyes

Julia Gunzenhäuser, Suliana Manley, Aleksandr Benke

Single particle tracking can reveal dynamic information at the scale of single molecules in living cells but thus far has been limited either in the range of potential protein targets or in the quality and number of tracks attainable. We demonstrate a new ...

American Chemical Society2012

Heterogeneity of AMPA receptor trafficking and molecular interactions revealed by superresolution analysis of live cell imaging

Suliana Manley, Christian Sieben

Simultaneous tracking of many thousands of individual particles in live cells is possible now with the advent of high-density superresolution imaging methods. We present an approach to extract local biophysical properties of cell-particle interaction from ...

Natl Acad Sciences2012

Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions

Julia Gunzenhäuser, Suliana Manley, Thomas Pengo

The HIV structural protein Gag assembles to form spherical particles of radius ∼70 nm. During the assembly process, the number of Gag proteins increases over several orders of magnitude from a few at nucleation to thousands at completion. The challenge in ...

American Chemical Society (ACS)2012

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