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Seamus John Holden

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Related publications (12)

Please note that this is not a complete list of this person’s publications. It includes only semantically relevant works. For a full list, please refer to Infoscience.

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Related publications (12)

Please note that this is not a complete list of this person’s publications. It includes only semantically relevant works. For a full list, please refer to Infoscience.

3D high-density localization microscopy using hybrid astigmatic/biplane imaging and sparse image reconstruction

Michaël Unser, Suliana Manley, Seamus John Holden, Lina Carlini

Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs ...

Optical Soc Amer2014

Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Suliana Manley, Seamus John Holden, Kyle Michael Douglass, Lina Carlini

Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, whi ...

Public Library of Science2015

FALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data

Michaël Unser, Suliana Manley, Cédric René Jean Vonesch, Hagay Kirshner, Seamus John Holden, Lina Carlini

Super resolution microscopy such as STORM and (F) PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow te ...

Nature Publishing Group2014