Publications by Isabelle Barde

Related publications (8)

Measuring In Vivo Protein Half-Life

Didier Trono, Kai Johnsson, Christian Heinis, Sandra Eloise Kjeldsen, Sonia Verp, Isabelle Barde, Karolina Bojkowska

Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident i ...

Elsevier2011

KRAB can repress lentivirus proviral transcription independently of integration site

Didier Trono, Isabelle Barde

The KRAB transcriptional repressor domain, commonly found in zinc finger proteins, acts by inducing the formation of heterochromatin. We previously exploited this property to achieve drug-regulated transgenesis and knock down by combining doxycycline-contr ...

2006

Release of human cytomegalovirus from latency by a KAP1/TRIM28 phosphorylation switch

Didier Trono, Adamantia Kapopoulou, Marco Cassano, Isabelle Barde, Benjamin Rauwel, Suk Min Jang

Human cytomegalovirus (HCMV) is a highly prevalent pathogen that induces life-long infections notably through the establishment of latency in hematopoietic stem cells (HSC). Bouts of reactivation are normally controlled by the immune system, but can be fat ...

2015

Regulation of Episomal Gene Expression by KRAB/KAP1-Mediated Histone Modifications

Patrick Aebischer, Didier Trono, Sonia Verp, Isabelle Barde, Christopher Towne, Vivianne Padrun, Anna Claire Groner

KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studi ...

American Society for Microbiology2009

Modulation of mitophagy and use thereof

Didier Trono, Isabelle Barde

The present invention relates to an agent or composition comprising at least one of: (i) a micro-RNA targeting a mitophagy-associated gene or a variant thereof, (ii) a vector comprising a nucleic acid encoding said micro-RNA or variant thereof, (iii) an in ...

2014

Control of mitophagie by microRNAs - A key step of erythropoiesis

Didier Trono, Jiri Vanicek, Sandra Eloise Kjeldsen, Sonia Verp, Adamantia Kapopoulou, Isabelle Barde, Ray Marcel Marin Florez, Benjamin Rauwel, Andrea Corsinotti

Edp Sciences S A2014

A KRAB/KAP1-miRNA Cascade Regulates Erythropoiesis Through Stage-Specific Control of Mitophagy

Didier Trono, Jiri Vanicek, Sandra Eloise Kjeldsen, Adamantia Kapopoulou, Isabelle Barde, Andrea Corsinotti, Ray Marcel Marin Florez, Benjamin Rauwel, Sonia Verp

During hematopoiesis, lineage- and stage-specific transcription factors work in concert with chromatin modifiers to direct the differentiation of all blood cells. Here, we explored the role of KRAB-containing zinc finger proteins (KRAB-ZFPs) and their cofa ...

American Association for the Advancement of Science2013

The questions you should ask yourself for efficient CRISPR/Cas9 genome editing

Sandra Eloise Kjeldsen, Isabelle Barde

Springer2016