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Theo Lasser

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Related publications (122)

Page 3 of 7

See the Brain at Work—Intraoperative Laser Doppler Functional Brain Imaging

Michaël Unser, Dimitri Nestor Alice Van De Ville, Theo Lasser, Marcel Leutenegger, Antonio Lopez, Erica Martin-Williams

During open brain surgery we acquire perfusion images non-invasively using laser Doppler imaging. The regions of brain activity show a distinct signal in response to stimulation providing intraoperative functional brain maps of remarkably strong contrast. ...

2009

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Theo Lasser, Kai Johnsson, Iwan Märki, Claudio Dellagiacoma, Stefan Geissbühler, Noelia Laura Bocchio, Grazvydas Lukinavicius, Sambashiva Banala

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010

Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements

Theo Lasser

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-col ...

SPIE Society of Photo-optical Instrumentation Engineers2005

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

Dimitri Nestor Alice Van De Ville, Theo Lasser, Kai Johnsson, Marcel Leutenegger, Iwan Märki, Boris Hinz, Aurélie Formey de Saint Louvent, Thiemo Spielmann, Matthias Geissbühler

Triplet imaging is a novel optical technique that allows investigating oxy- gen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolution, which are required for the monitoring of fast kinetics of ox ...

Springer Science+Business Media2012

Functional optical coherent imaging platform for medical application e.g. neurosurgery, has active camera unit that captures image of specific region of test subject after transmitting stimulus signals

Dimitri Nestor Alice Van De Ville, Theo Lasser, Marcel Leutenegger, Martin Villiger, Iwan Märki, Erica Martin-Williams

The present invention relates to a functional optical coherent imaging (fOCI) platform comprising at least one active camera unit (ACU) comprising a coherent and/or a partially coherent light source, and means for spectral filtering and imaging a selected ...

2010

Spectral cross-cumulants for multicolor super-resolved SOFI imaging

Theo Lasser, Aleksandra Radenovic, Marcel Leutenegger, Tomas Lukes, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer

Super-resolution opticalfluctuation imaging provides a resolution beyond the diffraction limitby analysing stochasticfluorescencefluctuations with higher-order statistics. Usingnthorderspatio-temporal cross-cumulants the spatial resolution and the sampling ...

Nature Research2020

Speckle statistics in optical coherence tomography

Theo Lasser, Boris Clément Karamata, Markus Laubscher, Kai Hassler

The two previously reported calculations of the amplitude distribution of speckles in optical coherence tomography, each based on a different mathematical formulation, yield different results. We show that a modification of an initial assumption in one of ...

2005

Fast focus field calculations

Theo Lasser, Marcel Leutenegger, Ramachandra Rao

We present a fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye diffraction integral is evaluated with the fast Fourier transform for calculat ...

2006

Reduction of Abeta amyloid pathology in APPPS1 transgenic mice in the absence of gut microbiota

Theo Lasser, Tristan Bolmont, Taoufiq Harach

Alzheimer’s disease is the most common form of dementia in the western world, however there is no cure available for this devastating neurodegenerative disorder. Despite clinical and experimental evidence implicating the intestinal microbiota in a number o ...

Nature Research2017

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Theo Lasser, Marcel Leutenegger, Noelia Laura Bocchio, Claudio Dellagiacoma, Corinne Berclaz

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

Dark field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

The invention relates to an optical coherence microscopy system for fast, phase resolved imaging by means of optical coherence microscopy with decoupled illumination and detection apertures, producing a dark-field effect with an enhanced optical contrast. ...

2011

Gastro-intestinal biomarkers for diagnosis and therapies of proteinopathies

Theo Lasser, Tristan Bolmont, Taoufiq Harach

Pharmaceutical composition for the treatment of proteinopathies comprising products which are able to stimulate growth or inhibition of at least one of Odoribacter, Oscillospira, Dehalobacterium, Alistipes, Parabacteroides, Lactobacillus and Sutterella, Fi ...

2016

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Theo Lasser, Aleksandra Radenovic, Tomas Lukes, Hendrik Georges Deschout, Lely Feletti, Azat Sharipov

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Spie-Int Soc Optical Engineering2017

Complex Ambiguity-Free Fourier Domain Optical Coherence Tomography through Transverse Scanning

Theo Lasser

We introduce a simple and cheap method for phase-shifting Fourier domain optical coherence tomography (FDOCT) that does not need additional devices and can easily be implemented. A small beam offset at the fast beam-scanning mirror introduces a causal phas ...

OSA2007

Diffraction-unlimited imaging: from pretty pictures to hard numbers

Theo Lasser, Marcel Leutenegger

Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts are now being focused on deriving quantitative ...

Springer Verlag2015

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Theo Lasser, Hilal Lashuel, Marcel Leutenegger, Emrah Bostan, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux, Anne-Laure Mahul Mellier

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Nature Publishing Group2018

In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

Theo Lasser, Anne Grapin-Botton, Martin Villiger, Joan Goulley

Aims/hypothesis Structural and functional imaging of the islets of Langerhans and the insulin-secreting beta cells represents a significant challenge and a long-lasting objective in diabetes research. In vivo microscopy offers a valuable insight into beta ...

Springer Verlag2009

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, François Aguet, Iwan Märki, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Vectorial reconstruction of retinal blood flow in three dimensions measured with high resolution resonant Doppler Fourier domain optical coherence tomography

Theo Lasser, Martin Villiger, Adrian Bachmann, Cédric Blatter

Resonant Doppler Fourier domain optical coherence tomography (FDOCT) is a functional imaging tool for extracting tissue flow. The method is based on the effect of interference fringe blurring in spectrometer-based FDOCT, where the path difference between s ...

SPIE Society of Photo-optical Instrumentation Engineers2007

Optical projection tomography for rapid whole mouse brain imaging

Dimitri Nestor Alice Van De Ville, Theo Lasser, Jérôme Extermann, Paul James Marchand, Antonio Lopez, Miguel Sison, David Nguyen, Arielle Louise Planchette, Jessica Sordet, Marcin Antoni Sylwestrzak

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of ...

Optical Soc Amer2017