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Theo Lasser

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Related publications (122)

Page 2 of 7

Visible light optical coherence correlation spectroscopy

Heinrich Hofmann, Theo Lasser, Marcel Leutenegger, Arno Pino Bouwens, Daniel Pawel Szlag, Lionel André Maurizi, Stéphane Broillet

Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a fact ...

Optical Society of America2014

Image formation and tomogram reconstruction in optical coherence microscopy

Theo Lasser, Martin Villiger

In this work we present a model for image formation in optical coherence microscopy. In the spectral domain detection, each wavenumber has a specific coherent transfer function that samples a different part of the object's spatial frequency spectrum. The r ...

2010

Compressed imaging by sparse random convolution

Theo Lasser, Aurélien Bourquard, Antonio Lopez

The theory of compressed sensing (CS) shows that signals can be acquired at sub-Nyquist rates if they are sufficiently sparse or compressible. Since many images bear this property, several acquisition models have been proposed for optical CS. An interestin ...

Optical Society of America2016

Dual-color total internal reflection fluorescence cross- correlation spectroscopy

Theo Lasser, Marcel Leutenegger

We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy (FCCS). As a performance analysis, we measur ...

SPIE Society of Photo-optical Instrumentation Engineers2006

Ultrasensitive reporter protein detection in genetically engineered bacteria

Theo Lasser

We demonstrate the use of laser-induced. fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates ...

2005

Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

Theo Lasser, Arno Pino Bouwens, Tristan Bolmont, Corinne Berclaz, Daniel Pawel Szlag

Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line ...

Optical Society of America2014

Statistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy

Vincent Khashayar Shamaei, Theo Lasser, Arno Pino Bouwens, Tristan Bolmont, Paul James Marchand, Daniel Pawel Szlag, David Nguyen, Jérôme Extermann

Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundre ...

Optical Soc Amer2017

High-speed laser Doppler perfusion imaging using an integrating CMOS image sensor

Theo Lasser

This paper describes the design and the performance of a new high-speed laser Doppler imaging system for monitoring blood flow over an area of tissue. The new imager delivers high-resolution flow images (256 x 256 pixels) every 2 to 10 seconds, depending o ...

2005

Prism-based multicolor fluorescence correlation spectrometer

Theo Lasser, Marcel Leutenegger

We report the design and application of a prism-based detection system for fluorescence (cross) correlation spectroscopy. The system utilizes a single laser wavelength for the simultaneous excitation of several dyes of different emission spectra. Fluoresce ...

2006

Simultaneous Multicolor Fluorescence Cross-Correlation Spectroscopy to Detect Higher Order Molecular Interactions Using Single Wavelength Laser Excitation

Theo Lasser

Fluorescence cross-correlation spectroscopy is a powerful method for the study of molecular interactions and dynamics in solution and even in living cells. Usually, in the optical setup, either two laser beams have to be superimposed in their respective co ...

2006

Imaging of G protein-coupled receptors in solid-supported planar lipid membranes

Theo Lasser, Marcel Leutenegger

This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform for biophysical studies. The authors introduced recently a nove ...

2009

Intensity based quantification of fast retinal blood flow in 3D via high resolution resonant Doppler spectral OCT

Theo Lasser, Martin Villiger, Adrian Bachmann, Cédric Blatter

Resonant Doppler Fourier Domain Optical Coherence Tomography is a functional imaging modality for quantifying fast tissue flow. The method profits from the effect of interference fringe blurring in spectrometer-based FDOCT in the presence of sample motion. ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2007

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Françoise Gisou van der Goot Grunberg, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

Full-field laser Doppler perfusion imaging and monitoring with an intelligent CMOS camera

Theo Lasser

A system for full-field laser Doppler blood flow imaging has been developed and tested on biomedical samples. The new imaging system allows 2D flow maps or monitoring flux signals to be obtained from a plurality of measured points simultaneously by using a ...

2005

Fabrication of arrays of sub-wavelength nano-apertures in an optically thick gold layer on glass slides for optical studies

Theo Lasser, Benjamin Dwir, Patrik Willi Hoffmann, Marcel Leutenegger, Ivo Utke, Alexandre Perentes

We report on the fabrication of two-dimensional arrays of nano-optical apertures in gold layers by electron beam lithography (EBL) on a transparent glass substrate. 30 x 30 μ m(2) large arrays of high aspect ratio sub-wavelength cylinders (400 nm diameter ...

2005

Heterodyne Fourier domain optical coherence tomography for full range probing with high axial resolution

Theo Lasser, Adrian Bachmann

One of the main drawbacks of Fourier domain optical coherence tomography (FDOCT) is the limited measurement depth range. Phase shifting techniques allow reconstructing the complex sample signal resulting in a doubled depth range. In current complex FDOCT r ...

2006

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics

Theo Lasser, Aleksandra Radenovic, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer, Jochem Deen

Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to p ...

2019

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Anne Grapin-Botton, Martin Villiger, Daniel Pawel Szlag, Arno Pino Bouwens, Corinne Berclaz, Joan Goulley, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Identification of luminescent molecules comprises fluorescence correlation spectroscopy

Theo Lasser, Radivoje Popovic, Pierre-André Besse, Alexis Rochas

The invention relates to a method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method. The inventive method comprising the following steps: a) preparing a sample (12) containing luminescent molecul ...

2005

High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis

Theo Lasser

Fluorescent nanodiamonds (fNDs) represent an emerging class of nanomaterials offering great opportunities for ultrahigh resolution imaging, sensing and drug delivery applications. Their biocompatibility, exceptional chemical and consistent photostability r ...

2019