Henning Paul-Julius Stahlberg

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The basic photosynthetic unit contg. the reaction center and the light-harvesting I complex (RC-LHI) of the purple non-sulfur bacterium Rhodospirillum rubrum was purified and reconstituted into two-dimensional (2D) membrane crystals. Transmission electron microscopy using conventional techniques and cryoelectron microscopy of the purified single particles and of 2D crystals yielded a projection of the RC-LHI complex at a resoln. of at least 1.6 nm. In this projection the LHI ring appears to have a square sy

The intricate process of α-synuclein aggregation and fibrillization holds pivotal roles in Parkinson’s disease (PD) and multiple system atrophy (MSA). While mouse α-synuclein can fibrillize in vitro, whether these fibrils commonly used in research to induc ...

Lewy bodies, the defining pathological feature of Parkinson’s disease, are intraneuronal inclusions enriched in aggregated alpha-synuclein (αSyn). We used correlative light and electron microscopy to selectively investigate phosphorylated αSyn (αSyn pS129 )-positive inclusions in the substantia nigra of end-stage postmortem Parkinson’s disease brain. Here we show that somatic αSyn pS129 inclusions in nigral dopaminergic neurons are consistently fibrillar, whereas the membranous-type inclusions are restricte

The minimal photounit of the photosynthetic membranes of the purple non-sulfur bacterium Rhodospirillum rubrum, comprising the reaction center and the light-harvesting complex has been purified and crystd. in two dimensions in the presence of added phospholipids, and subsequently visualized by electron microscopy after neg.-staining. The position of the reaction centers within the light-harvesting ring has been detd. at low resoln. by the application of a new anal. for rotationally disordered identical unit

Grayscale electron beam lithography (g-EBL) is a fabrication technique that allows for tunable control of resist topography. In most cases, the height of the structures is in the submicron regime. Here, we present an extensive experimental characterization ...

ExbBD is part of a cytoplasmic membrane molecular motor driven by the proton-motive force. It belongs to the larger family of motors involved in nutriment import across the outer membrane of Gram-negative bacteria (ExbBD), flagellar rotation (MotAB) or lat ...

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly un ...

ABCG2 is a multidrug transporter that affects drug pharmacokinetics and contributes to multidrug resistance of cancer cells. In previously reported structures, the reaction cycle was halted by the absence of substrates or ATP, mutation of catalytic residue ...

The rapid evolution of SARS-CoV-2 to variants with improved transmission efficiency and reduced sensitivity to vaccine-induced humoral immunity has abolished the protective effect of licensed therapeutic human monoclonal antibodies (mAbs). To fill this unmet medical need and protect vulnerable patient populations, we isolated the P4J15 mAb from a previously infected, vaccinated donor, with

The careful design of the antibody architecture is becoming more and more important, especially when the purpose is agonism. We present the design of a novel antibody format that is able to promote receptor dimerization and induce signal transduction resul ...

Transient receptor potential melastatin 4 (TRPM4) is a Ca 2+ −activated cation channel whose pharmacology is shaped by its molecular environment. It remains poorly understood how temperature and membrane context influence inhibitor recognition. Here we combine cryo−electron microscopy of membrane-derived vesicles and detergent−solubilized TRPM4 to investigate lipid−associated architecture and binding of the potent anthranilic anilide inhibitor PBA. We find that membrane vesicles preserve a native−like paral

Several systems, including contractile tail bacteriophages, the type VI secretion system and R-type pyocins, use a multiprotein tubular apparatus to attach to and penetrate host cell membranes. This macromolecular machine resembles a stretched, coiled spri ...

Focal adhesion kinase (FAK) is a key component of the membrane proximal signaling layer in focal adhesion complexes, regulating important cellular processes, including cell migration, proliferation, and survival. In the cytosol, FAK adopts an autoinhibited ...

Due to the rapid evolution of SARS-CoV-2 to variants with reduced sensitivity to vaccine-induced humoral immunity and the near complete loss of protective efficacy of licensed therapeutic monoclonal antibodies, we isolated a potent, broad-spectrum neutrali ...

Amyloid polymorphism is a hallmark of almost all amyloid species, yet the mechanisms underlying the formation of amyloid polymorphs and their complex architectures remain elusive. Commonly, two main mesoscopic topologies are found in amyloid polymorphs cha ...

Injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the first three-dimensional structure of Yersinia enterocolitica ...

ABCG2 is a multidrug transporter that protects tissues from xenobiotics, affects drug pharmacokinetics, and contributes to multidrug resistance of cancer cells. Here, we present tetracyclic fumitremorgin C analog Ko143 derivatives, evaluate their in vitro ...

Interphotoreceptor retinoid‐binding protein (IRBP) is a highly expressed protein secreted by rod and cone photoreceptors that has major roles in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retin ...

With the advent of modern technologies for cryo-electron tomography (cryo-ET), high-quality tilt series are more rapidly acquired than processed and analyzed. Thus, a robust and fast-automated alignment for batch processing in cryo-ET is needed. While diff ...

Fibrils of alpha-synuclein are significant components of cellular inclusions associated with several neuropathological disorders including Parkinson’s disease, multiple system atrophy and dementia with Lewy bodies. In recent years, technological advances i ...

Henning Paul-Julius Stahlberg

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Are the light-harvesting I complexes from Rhodospirillum rubrum arranged around the reaction center in a square geometry?

Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases

Ultrastructural diversity and subcellular organization of nigral Lewy pathology in Parkinson’s disease

The reaction center of the photounit of Rhodospirillum rubrum is anchored to the light-harvesting complex with four-fold rotational disorder

Grayscale e-beam lithography: Effects of a delayed development for well-controlled 3D patterning

Functional and structural characterization of Serratia marcescens ExbB: determinants of the interaction with HasB/TonB

X-ray structure of the mouse serotonin 5-HT3 receptor

Structures of ABCG2 under turnover conditions reveal a key step in the drug transport mechanism

ACE2 mimetic antibody potently neutralizes all SARS-CoV-2 variants and fully protects in XBB.1.5 challenged monkeys

The Contorsbody, an antibody format for agonism: design, structure, and function

Temperature-dependent ligand relocation reveals plasticity of TRPM4 inhibition

Structure of the T4 baseplate and its function in triggering sheath contraction

Structural basis of Focal Adhesion Kinase activation on lipid membranes

Broadly potent anti-SARS-CoV-2 antibody shares 93% of epitope with ACE2 and provides full protection in monkeys

Hierarchical Protofilament Intertwining Rules the Formation of Mixed‐Curvature Amyloid Polymorphs

In situ structural analysis of the Yersinia enterocolitica injectisome

Modulation of ABCG2 Transporter Activity by Ko143 Derivatives

Single particle cryo‐EM of the complex between interphotoreceptor retinoid‐binding protein and a monoclonal antibody

Automated fiducial-based alignment of cryo-electron tomography tilt series in Dynamo

New insights on the structure of alpha-synuclein fibrils using cryo-electron microscopy