Rudolf Hovius

Expertise •Protein structure, expression & purification•Molecular interaction & receptor activation•Fluorescence imaging & spectroscopySignalling & sensing Mission => Teaching: Lecture courses, tutorials and practicals in biochemistry, biophysical chemistry of membranes and signalling processes. Participating to the Commission d'Enseignement and Commission de l'Ecole Doctorale of Chemistry and Chemical Engineering.=> Research interests: Protein function and structure, Membrane protein (especially receptors and channels) expression, structure and function, biomolecular interaction, labeling with biophysical probes, fluorescence imaging and assay and sensor techniques, single molecule techniques. BSc course EPFL : Practical BioChem & BioPhys Practical introducing 6th Chemistry Bachelor students in genetic engineering, protein expression and purification, and biochemical and biophysical charaterisation of proteins.BSc course Molecular and cellular biophysics II .Graduate course : ESONN - Grenoble Module "Advanced biophysics for microsystems design" at the post-graduate European School on Nanosciences & Nanotechnologies (ESONN.fr) in Grenoble, organized by the IPNG, UJF, CNRS and CEA.MSc course : Cellular signalling Masters course on advanced concepts of various cellular signalling pathways and their interactionsMSc Course Chemical Biology . ResearchPatents New conjugate of agonist ligand directed to ligand-gated ion channels of cysteine loop-family with chromophore, useful, e.g. in receptor proteins/their interaction with agonistic ligand and to study the effect of unlabelled ligands.J. Grandl, R. Hovius, H. Vogel, D. Bertrand, E. Sakr, F. Kotzyba-Hibert and M. Goeldner. Patent number: WO2007057428-A1 Publication date: 2007Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics .C. Schreiter, J.-M. Segura, H. Vogel, R. Hovius, K. Martinez and M. Gjoni Boillat. Patent number: WO2006105985-A1 Publication date: 2006Current interests Structure and function DNA-protein macromolecular complexesReal-time monitoring of structural dynamics and molecular interactions Single molecule techniques & cellular signalling Teaching & PhD Past EPFL PhD Students as codirector Joachim Piguet Courses Experimental biochemistry and biophysics CH-319 A 7-week long (4+8 h) experiment where you plan and construct a fluorescent sensor protein starting from DNA bricks. The protein will be expressed in and purified from E.coli, characterized by biochemical and spectroscopic methods. Your report will be in the style of a scientific paper or poster ESONN Graduate course : ESONN - GrenobleModule "Advanced biophysics for microsystems design" at the post-graduate European School on Nanosciences & Nanotechnologies (ESONN.fr) in Grenoble, organized by the IPNG, UJF, CNRS and CEA.