Mediaspace scheduled maintenance: Aug 25, 2026 07:00 - 12:00 AM. During this time, videos will be temporarily unavailable. Check status updates.

Graph
Search

Course

CH-319: Experimental biochemistry and biophysics

Summary

A 7-week long (4+8 h) experiment where you plan and construct a fluorescent sensor protein starting from DNA bricks. The protein will be expressed in and purified from E.coli, characterized by biochemical and spectroscopic methods. Your report will be in the style of a scientific paper or poster

Lectures

Instructor

Rudolf Hovius

Official source

https://edu.epfl.ch/coursebook/en/experimental-biochemistry-and-biophysics-CH-319

Moodle Page

http://go.epfl.ch/CH-319

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Summary

Lectures

Instructor

Rudolf Hovius

Official source

https://edu.epfl.ch/coursebook/en/experimental-biochemistry-and-biophysics-CH-319

Moodle Page

http://go.epfl.ch/CH-319

About this result

Ontological neighbourhood

Biology

Molecular biology: Topics in molecular biology

Biological engineering

Biomedical engineering: Genetic engineering

Lectures in this course (10)

PCR and Restriction Cloning

Covers genetic engineering, FRET sensors, PCR cloning, and DNA constructs for practical applications.

Circular Permutation in Protein Engineering

Delves into circular permutation in protein engineering, focusing on optimizing proteins by introducing new termini without changing the structure.

Cloning Projects Analysis

Explores the use of ApE for planning and analyzing cloning projects.

Protein Expression Systems

Explores protein expression systems, vector design, and solubility enhancement in E. coli and other common systems.

Plasmid Transformation and DNA Isolation

Covers transforming plasmid DNA into bacteria and isolating DNA from bacterial colonies.

Related courses (31)

BIO-212: Biological chemistry I

Biochemistry is a key discipline for the Life Sciences. Biological Chemistry I and II are two tightly interconnected courses that aim to describe and understand in molecular terms the processes that make life possible. ...

CH-210: Biochemistry

Les constituants biochimiques de l'organisme, protéines, glucides, lipides, à la lumière de l'évolution des concepts et des progrès en biologie moléculaire et génétique, sont étudiés. ...

BIO-204: Integrated labo in Life sciences II

Au cours de deux semestres, vous utilisez la biologie moléculaire, la biologie cellulaire ainsi que la biochimie pour cloner un ADNc dans un plasmide d'expression, afin de produire, purifier et caractériser la protéine recombinante. ...

BIO-203: Integrated labo in Life sciences I

BIOENG-320: Synthetic biology

This advanced Bachelor/Master level course will cover fundamentals and approaches at the interface of biology, chemistry, engineering and computer science for diverse fields of synthetic biology. This class requires critical and analytical thinking at the ...