Course

CH-319: Experimental biochemistry and biophysics

Summary

A seven-week laboratory course (4 h+8 h per week) in which students construct, modify and screen microbial sensor strains from defined DNA parts. Proteins are heterologously expressed and purified, then characterized biochemically and spectroscopically. Final reporting in scientific article format.

Lectures

Instructor

Rudolf Hovius

Expertise Â•Protein structure, expression & purificationÂ•Molecular interaction & receptor activationÂ•Fluorescence imaging & spectroscopySignalling & sensing Mission => Teaching: Lecture courses, tutorials and practicals in biochemistry, biophysical chemistry of membranes and signalling processes. Participating to the Commission d'Enseignement and Commission de l'Ecole Doctorale of Chemistry and Chemical Engineering.=> Research interests: Protein function and structure, Membrane protein (especially recepto

Official source

https://edu.epfl.ch/coursebook/en/experimental-biochemistry-and-biophysics-CH-319

Moodle Page

http://go.epfl.ch/CH-319

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Summary

Lectures

Instructor

Rudolf Hovius

Official source

https://edu.epfl.ch/coursebook/en/experimental-biochemistry-and-biophysics-CH-319

Moodle Page

http://go.epfl.ch/CH-319

About this result

Lectures in this course (10)

PCR and Restriction Cloning

Covers genetic engineering, FRET sensors, PCR cloning, and DNA constructs for practical applications.

Circular Permutation in Protein Engineering

Delves into circular permutation in protein engineering, focusing on optimizing proteins by introducing new termini without changing the structure.

Cloning Projects Analysis

Explores the use of ApE for planning and analyzing cloning projects.

Protein Expression Systems

Explores protein expression systems, vector design, and solubility enhancement in E. coli and other common systems.

Plasmid Transformation and DNA Isolation

Covers transforming plasmid DNA into bacteria and isolating DNA from bacterial colonies.

Related courses (31)

BIO-212: Biological chemistry I

Biochemistry is a key discipline for the Life Sciences. Biological Chemistry I and II are two tightly interconnected courses that aim to describe and understand in molecular terms the processes that make life possible. ...

CH-210: Biochemistry

Les constituants biochimiques de l'organisme, protéines, glucides, lipides, à la lumière de l'évolution des concepts et des progrès en biologie moléculaire et génétique, sont étudiés. ...

BIO-204: Integrated labo in Life sciences II

Au cours de deux semestres, vous utilisez la biologie moléculaire, la biologie cellulaire ainsi que la biochimie pour cloner un ADNc dans un plasmide d'expression, afin de produire, purifier et caractériser la protéine recombinante. ...

BIO-203: Integrated labo in Life sciences I

BIOENG-320: Synthetic biology

This advanced Bachelor/Master level course will cover fundamentals and approaches at the interface of biology, chemistry, engineering and computer science for diverse fields of synthetic biology. This class requires critical and analytical thinking at the ...