Publications associées à Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models

Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos

Constitutive heterochromatin is essential for transcriptional silencing and genome integrity. The establishment of constitutive heterochromatin in early embryos and its role in early fruitfly development are unknown. Lysine 9 trimethylation of histone H3 ( ...

Springernature2024

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos

Pierre Gönczy, Fernando Romero Balestra

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the ...

GENETICS SOCIETY AMERICA2023

The nuclear-to-cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arctica

Marine Olivetta, Omaya Pierre Dudin

The ratio of nuclear content to cytoplasmic volume (N/C ratio) is a key regulator driving the maternal-to -zy-gotic transition in most animal embryos. Altering this ratio often impacts zygotic genome activation and de-regulates the timing and outcome of em ...

CELL PRESS2023

Species-specific regulation of XIST by the JPX/FTX orthologs

Olga Marie Louise Rosspopoff

X chromosome inactivation (XCI) is an essential process, yet it initiates with remarkable diversity in various mammalian species. XIST, the main trigger of XCI, is controlled in the mouse by an interplay of lncRNA genes (LRGs), some of which evolved concom ...

OXFORD UNIV PRESS2023

Targeted search for scaling genes reveals matrix metalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos

Daria Korotkova

How embryos scale patterning according to size is still not fully understood. Through in silico screening and analysis of reaction-diffusion systems that could be responsible for scaling, we predicted the existence of genes whose expression is sensitive to ...

Cell Press - Elsevier2022

NMR spectroscopy of a single mammalian early stage embryo

Giovanni Boero, Jürgen Brugger, Marco Grisi, Gaurasundar Marc Conley, Kyle Joel Rodriguez

The resolving power, chemical sensitivity and non-invasive nature of NMR have made it an established technique for in vivo studies of large organisms both for research and clinical applications. NMR would clearly be beneficial for analysis of entities at t ...

Academic Press Inc - Elsevier Science2022

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Stefano Davide Vianello

In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...

EPFL2022

A computational modeling approach to study the neuromechanics of terrestrial locomotion

Shravan Tata Ramalingasetty

The main theme of my thesis will be to use neuro-muscular modeling techniques to study locomotion of terrestrial mammals. Locomotion is the ability of animals to interact with the environment to propel themselves in space. It involves co-ordination and pre ...

EPFL2022

KRAB zinc finger protein-mediated control of transposon-embedded regulatory sequences from human germline to early embryos

Alexandra Iouranova

Transposable elements (TEs), also called jumping genes, are genetic elements capable of changing their position within the genome of their host. They make up large fractions of genomes, including 45% of human DNA content, according to current estimates. S ...

EPFL2021

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Gijs, Matteo Cornaglia, Hüseyin Baris Atakan, Tunç Alkanat, Raphaël Etienne Jean Trouillon

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

Unequal cleavage of the C. elegans zygote ensures robust embryogenesis

Radek Jankele

Asymmetric cell divisions play key roles in generating cellular diversity during embryogenesis, and in tissue homeostasis during postembryonic life. Particular asymmetric cell divisions producing differentially sized daughter cells were described in many s ...

EPFL2020

Fractal Analysis And Related Forms Of Complexity Of Embryo Development. Is There A New Tool For Human Embryo Selection?

Maria-Alexandra Paun

A total number of 1092 embryos, originated from 168 couples that underwent IVF cycles, were analyzed in this study. The embryos were cultivated in a continuous culture and monitored using the time lapse system EmbryoScope. The microscopic images of the fer ...

UNIV POLITEHNICA BUCHAREST, SCI BULL2019

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Gijs, Elena Katsyuba, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Thomas Lehnert, Martinus Gijs, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids

Matthias Lütolf, Denis Duboule, Leonardo Beccari, Mehmet Ugur Girgin, Anne-Catherine Cossy, Alfonso Martinez Arias

The emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordina ...

NATURE PUBLISHING GROUP2018

DUX-family transcription factors regulate zygotic genome activation in placental mammals

Didier Trono, Sonia Verp, Julien Léonard Duc, Andrea Coluccio, Alberto De Iaco, Evaristo Jose Planet Letschert

In animal embryos, transcription is mostly silent for several cell divisions, until the release of the first major wave of embryonic transcripts through so-called zygotic genome activation (ZGA). Maternally provided ZGA-triggering factors have been identif ...

2017

Segmentation of Developing Human Embryo in Time-lapse Microscopy

Mathieu Salzmann

Being able to efficiently segment a developing embryo from background clutter constitutes an important step in automated monitoring of human embryonic cells. State-of-the-art automatic segmentation methods remain ill-suited to handle the complex behavior a ...

IEEE2016

Molecular Criteria for Defining the Naive Human Pluripotent State

Didier Trono, Julien Léonard Duc, Marc Samuel Friedli, Alexandra Iouranova, Michaël Imbeault, Evaristo Jose Planet Letschert, Julien Paul André Pontis, Haoyi Wang

Recent studies have aimed to convert cultured human pluripotent cells to a naive state, but it remains unclear to what extent the resulting cells recapitulate in vivo naive pluripotency. Here we propose a set of molecular criteria for evaluating the naive ...

Cell Press2016

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Royal Soc Chemistry2016

Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification

Daniel Constam, Winship Herr, Sylvain Frédéric Bessonnard

Mammalian Host-Cell Factor 1 (HCF-1), a transcriptional co-regulator, plays important roles during the cell-division cycle in cell culture, embryogenesis as well as adult tissue. In mice, HCF-1 is encoded by the X-chromosome-linked Hcfc1 gene. Induced Hcfc ...

Academic Press Inc Elsevier Science2016