Publications associées à Planar Cell Polarity Controls Pancreatic Endocrine Differentiation

Bioengineering functional organoid models across scales

The last two decades have seen the development of organoid models for many different tissues and organs. Organoids are three-dimensional organ-mimetics derived from stem or progenitor cells comprising various specialized cell types, resembling the architec ...

EPFL2024

Characterization of the gut-bone marrow axis through bile acid signaling

Alejandro Alonso Calleja

Communication between the intestine and other organs such as the lungs, brain or bones is mediated by several metabolites, like short-chain fatty acids or bile acids, that relay information about nutritional and microbiota status. Bile acids are endogenous ...

EPFL2024

Disruption of stem cell niche-confined R-spondin 3 expression leads to impaired hematopoiesis

Freddy Radtke, Ute Koch, Jialin Zhang, Soufiane Boumahdi

Self-renewal and differentiation of stem and progenitor cells are tightly regulated to ensure tissue homeostasis. This regulation is enabled both remotely by systemic circulating cues, such as cytokines and hormones, and locally by various niche-confined f ...

ELSEVIER2023

Cross-anatomical single-cell definition and characterization of human adipose progenitor niche

Radiana Ferrero

Adipose tissue is a key metabolic and highly-dynamic organ whose dysregulation may cause clinical conditions of concern such as obesity and lipodystrophy. Its function varies based on the anatomy, in fact, visceral adipose tissue expansion carries a greate ...

EPFL2023

Neuronal migration prevents spatial competition in retinal morphogenesis

Martin Weigert

The concomitant occurrence of tissue growth and organization is a hallmark of organismal development(1-3). This often means that proliferating and differentiating cells are found at the same time in a continuously changing tissue environment. How cells ada ...

NATURE PORTFOLIO2023

Clonal relations in the mouse brain revealed by single-cell and spatial transcriptomics

Gioele La Manno

Ratz et al. present an easy-to-use method to barcode progenitor cells, enabling profiling of cell phenotypes and clonal relations using single-cell and spatial transcriptomics, providing an integrated approach for understanding brain architecture. ...

NATURE PORTFOLIO2022

New approaches to uncover the minimally functional hematopoietic niche using the adipocytic differentiation axis: pharmacological modulation and adrenal niche induction

Frédérica Schyrr

Hematopoietic Stem and Progenitor Cells (HSPCs) reside in their niche, a structure that regulates the balance of cellular quiescence, self-renewal and commitment towards differentiated cells. This highly plastic niche is formed by several cellular players, ...

EPFL2022

Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence

Yann Barrandon, Stéphanie Claudinot, Paola Bonfanti, Ariane Rochat, Daniel-Alexandre Littman, Christèle Gonneau

The formation of hair follicles, a landmark of mammals, requires complex mesenchymal-epithelial interactions and it is commonly believed that embryonic epidermal cells are the only cells that can respond to hair follicle morphogenetic signals in vivo. Here ...

Nature Research2020

PEG/HA Hybrid Hydrogels for Biologically and Mechanically Tailorable Bone Marrow Organoids

Martin Ehrbar, Queralt Vallmajó Martín, Nicolas Antoine Vincent Broguiere

Bone marrow (BM) organoids provide powerful tools to study the vital interplay between the BM microenvironment and resident cells. Current biomaterials, however, are limited in terms of versatility for independently studying the biochemical and biophysical ...

WILEY-V C H VERLAG GMBH2020

Polarised three-dimensional cellular aggregates

Matthias Lütolf, Giuliana Rossi

Polarised three-dimensional cellular aggregates (or gastruloids) generated in vitro from one or more pluripotent stem cells are provided. Methods for obtaining polarised three-dimensional cellular aggregates and cells (e.g. progenitor cells and derivatives ...

2020

Bioengineering human bone marrow models to elucidate the triggers of the stem cell niche

Queralt Vallmajó Martín

The bone marrow (BM) microenvironment, or niche, significantly affects behaviors of its resident stem cell populations. Disruptions in the BM niche contribute to a number of severe clinical pathologies. Discovery of novel therapeutic strategies for BM-rela ...

EPFL2019

Metabolic and microenvironmental strategies to accelerate hematopoietic recovery post-aplasia

Vasco Ledebur de Antas de Campos

The procedure-associated mortality rate of bone marrow transplantations (BMT) remains close to 25%. The BMT is therefore only indicated for life-threatening diseases, with no replacement of this technology to be expected in the foreseeable future. Modest i ...

EPFL2019

Modeling hematopoietic stem cell dynamics in bioengineered niches

Sonja Giger

Bone marrow transplantation is a well-established medical procedure for the treatment of various hematologic diseases. However, the relatively low number of hematopoietic stem cells (HSCs) that can be harvested, especially from umbilical cord blood, limits ...

EPFL2019

An extended regulatory landscape drives Tbx18 activity in a variety of prostate-associated cell lineages

Christopher Chase Bolt, Soumya Negi

The evolutionarily conserved transcription factor, Tbx18, is expressed in a dynamic pattern throughout embryonic and early postnatal life and plays crucial roles in the development of multiple organ systems. Previous studies have indicated that this dynami ...

ACADEMIC PRESS INC ELSEVIER SCIENCE2019

Effect of temporal onsets of mechanical loading on bone formation inside a tissue engineering scaffold combined with cell therapy

Dominique Pioletti, Tanja Cloé Hausherr

Several approaches to combine bone substitutes with biomolecules, cells or mechanical loading have been explored as an alternative to the limitation and risk-related bone auto- and allo-grafts. In particular, human bone progenitor cells seeded in porous po ...

2018

Autologous Cell Therapy Approach for Duchenne Muscular Dystrophy using PiggyBac Transposons and Mesoangioblasts

Nicolas Mermod

Duchenne muscular dystrophy (DMD) is a lethal muscle-wasting disease currently without cure. We investigated the use of the PiggyBac transposon for full-length dystrophin expression in murine mesoangioblast (MABs) progenitor cells. DMD murine MABs were tra ...

CELL PRESS2018

Human bone progenitor cells for clinical application: what kind of immune reaction does fetal xenograft tissue trigger in immuno-competent rats?

Dominique Pioletti, Tanja Cloé Hausherr, Lee Ann Laurent-Applegate

The potential of human fetal bone cells for successful bone regeneration has been shown in vivo. In particular, it has been demonstrated that the seeding of these cells in porous poly-(L-lactic acid)/ ß-tricalcium phosphate scaffolds improved the bone form ...

Cognizant Communication Corp2017

Outside-in integrin signalling regulates haematopoietic stem cell function via Periostin-Itgav axis

Jörg Hülsken, Albert Santamaria Martinez

Integrins play an important role in haematopoietic stem cell (HSC) maintenance in the bone marrow niche. Here, we demonstrate that Periostin (Postn) via interaction with Integrin-alpha v (Itgav) regulates HSC proliferation. Systemic deletion of Postn resul ...

Nature Publishing Group2016

In vivo mechanical loading combined with cell therapy in a bone tissue engineering scaffold

Tanja Cloé Hausherr

Bone defects generate a worldwide high demand for bone repair and reconstruction. The incidence of bone defect repair in our aging society is expected to increase in the next decades. As bone auto- and allografting solutions often suffer from limited suppl ...

EPFL2016

Cell Cycle-Dependent Differentiation Dynamics Balances Growth and Endocrine Differentiation in the Pancreas

Anne Grapin-Botton, Laurence Anne E Lemaire, Yung Hae Kim

Organogenesis relies on the spatiotemporal balancing of differentiation and proliferation driven by an expanding pool of progenitor cells. In the mouse pancreas, lineage tracing at the population level has shown that the expanding pancreas progenitors can ...

Public Library Science2015