Publications associées à Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis

Metabolic dependencies of metastasis-initiating cells in female breast cancer

Jörg Hülsken, Albert Santamaria Martinez, Pierre Vincent Dessen, Laurent Nam Ky Beziaud, Candice Megan T Young, Angela Madurga Alonso

Understanding the mechanisms that enable cancer cells to metastasize is essential in preventing cancer progression. Here we examine the metabolic adaptations of metastasis-initiating cells (MICs) in female breast cancer and how those shape their metastatic ...

Nature Portfolio2023

Enabling Stroke Theranostics with Hyperpolarized Magnetic Resonance

Thanh Phong Kevin Lê

The severe social and economic burden of stroke is a strong motivation for the exploration of innovative medical approaches. In this context, magnetic resonance (MR) of hyperpolarized (HP) molecular agents via dissolution dynamic nuclear polarization (dDNP ...

EPFL2023

Detection of myocardial medium‐chain fatty acid oxidation and tricarboxylic acid cycle activity with hyperpolarized [1– 13 C]octanoate

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara

Under normal conditions, the heart mainly relies on fatty acid oxidation to meet its energy needs. Changes in myocardial fuel preference are noted in the diseased and failing heart. The magnetic resonance signal enhancement provided by spin hyperpolarizati ...

2020

PHD3 Loss Promotes Exercise Capacity and Fat Oxidation in Skeletal Muscle

Daniel Garcia, Johan Auwerx, Hao Li

Rapid alterations in cellular metabolism allow tissues to maintain homeostasis during changes in energy availability. The central metabolic regulator acetyl-CoA carboxylase 2 (ACC2) is robustly phosphorylated during cellular energy stress by AMP-activated ...

Cell Press2020

A Single Metabolite which Modulates Lipid Metabolism Alters Hematopoietic Stem/Progenitor Cell Behavior and Promotes Lymphoid Reconstitution

Matthias Lütolf, Marlen Knobloch, Mergim Ramosaj, Sonja Giger

Fatty acid beta-oxidation (FAO), the breakdown of lipids, is a metabolic pathway used by various stem cells. FAO levels are generally high during quiescence and downregulated with proliferation. The endogenous metabolite malonyl-CoA modulates lipid metabol ...

2020

In vivo detection of d-amino acid oxidase with hyperpolarized d-[1-C-13]alanine

Rolf Gruetter, Hikari Ananda Infinity Yoshihara, Alice Radaelli

d-amino acid oxidase (DAO) is a peroxisomal enzyme that catalyzes the oxidative deamination of several neutral and basic d-amino acids to their corresponding alpha-keto acids. In most mammalian species studied, high DAO activity is found in the kidney, liv ...

2020

CDK4 Phosphorylates AMPK alpha 2 to Inhibit Its Activity and Repress Fatty Acid Oxidation

Anita Nasrallah, Caterina Collodet, Judit Castillo Armengol

The roles of CDK4 in the cell cycle have been extensively studied, but less is known about the mechanisms underlying the metabolic regulation by CDK4. Here, we report that CDK4 promotes anaerobic glycolysis and represses fatty acid oxidation in mouse embry ...

Cell Press2017

Mitochondrial Post-translational Modifications and Metabolic Control: Sirtuins and Beyond

Carlos Canto Alvarez

Introduction: In order to maintain metabolic homeostasis, organisms adjust the capacity and efficiency of ATP generation to changes in energetic demand and supply. While the transcriptional control of mitochondrial biogenesis allows to adapt mitochondrial ...

Bentham Science Publ Ltd2017

Energy metabolism in the rat cortex under thiopental anaesthesia measured

Rolf Gruetter, João Miguel das Neves Duarte, Nathalie Just, Sarah Catherine Sonnay

Barbiturates, commonly used as general anaesthetics, depress neuronal activity and thus cerebral metabolism. Moreover, they are likely to disrupt the metabolic support of astrocytes to neurons, as well as the uptake of nutrients from circulation. By employ ...

Wiley-Blackwell2017

Hyperpolarized Magnetic Resonance Methods to Investigate Metabolic Pathways in Liver and Immune Cells

Emine Can

Dysregulation of cellular metabolism is a key feature of major diseases such as diabetes and cancer. Monitoring cellular metabolic alterations is of critical importance for improved understanding of disease progression and the development of novel therapeu ...

EPFL2017

A rheostat mechanism governs the bifurcation of carbon flux in mycobacteria

John McKinney, Florence Pojer, Matteo Dal Peraro, Tarun Chopra, Paul Murima, Giulia Fonti

Fatty acid metabolism is an important feature of the pathogenicity of Mycobacterium tuberculosis during infection. Consumption of fatty acids requires regulation of carbon flux bifurcation between the oxidative TCA cycle and the glyoxylate shunt. In Escher ...

Nature Publishing Group2016

Measuring changes in substrate utilization in the myocardium in response to fasting using hyperpolarized [1-(13)C]butyrate and [1-(13)C]pyruvate

Arnaud Comment, Josefina Adriana Maria Bastiaansen

Cardiac dysfunction is often associated with a shift in substrate preference for ATP production. Hyperpolarized (HP) (13)C magnetic resonance spectroscopy (MRS) has the unique ability to detect real-time metabolic changes in vivo due to its high sensitivit ...

Nature Research2016

Deciphering the Role of LRH-1 in Liver Intermediary Metabolism and Cancer

Pan Xu

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death worldwide. Gaining insight into the molecular pathways involved in the development of HCC is hence a prerequisite to design and develop novel and effective therapeutic strategies. ...

EPFL2016

LRH-1-dependent programming of mitochondrial glutamine processing drives liver cancer

Arnaud Comment, Matthias Alexander Sokrates Stein, Kristina Schoonjans, Johan Auwerx, Norman Lionel Moullan, Dongryeol Ryu, Maaike Hélène Oosterveer, Emine Can, Pan Xu, Xu Wang, Hadrien Charles Edouard Demagny

Various tumors develop addiction to glutamine to support uncontrolled cell proliferation. Here we identify the nuclear receptor liver receptor homolog 1 (LRH-1) as a key regulator in the process of hepatic tumorigenesis through the coordination of a noncan ...

Cold Spring Harbor Laboratory2016

Brain energy metabolism measured by 13C magnetic resonance spectroscopy in vivo upon infusion of [3-13C]lactate

Rolf Gruetter, João Miguel das Neves Duarte, Freya-Merret Girault

The brain uses lactate produced by glycolysis as an energy source. How lactate originated from the blood stream is used to fuel brain metabolism is not clear. The current study measures brain metabolic fluxes and estimates the amount of pyruvate that becom ...

Wiley-Blackwell2015

Characterization of hepatic fatty acids in mice with reduced liver fat by ultra-short echo time (1) H-MRS at 14.1 T in vivo

Rolf Gruetter, Hongxia Lei, Ana Francisca Leal Silva Soares

Alterations in the hepatic lipid content (HLC) and fatty acid composition are associated with disruptions in whole body metabolism, both in humans and in rodent models, and can be non-invasively assessed by (1) H-MRS in vivo. We used (1) H-MRS to character ...

Wiley-Blackwell2015

Deciphering the astrocyte-neuron metabolic cooperation in vivo

Manuel Raphael Zenger

Brain energy consumption is high and a very tightly regulated energy metabolism ensures correct performance. Neurons, the cells responsible for transmitting information via synapses, interact very closely with star shaped glial cells called astrocytes. Ast ...

EPFL2015

Facile synthesis of efficient visible active C-doped TiO2 nanomaterials with high surface area for the simultaneous removal of phenol and Cr(VI)

Nicolas Xanthopoulos

A single step synthesis of carbon doped TiO2 (anatase) nanomaterials have been reported by using combustion synthesis using ascorbic acid and citric acid fuels. X-ray diffraction studies indicated the formation of nanosized anatase titania, whereas, transm ...

Pergamon-Elsevier Science Ltd2015

Quantitative Mass Spectrometry Reveals Plasticity of Metabolic Networks in Mycobacterium smegmatis

Marc Moniatte, Diego Chiappe, John McKinney, Tarun Chopra, Florence Armand, Romain Hamelin

Mycobacterium tuberculosis has a remarkable ability to persist within the human host as a clinically inapparent or chronically active infection. Fatty acids are thought to be an important carbon source used by the bacteria during long term infection. Catab ...

Amer Soc Biochemistry Molecular Biology Inc2014

Regulation of Energy Metabolism and Mitochondrial Function in Skeletal Muscle During Lipid Overfeeding in Healthy Men

Context/Objective: The aim of this study was to evaluate the regulation of the fuel partitioning and energy metabolism in skeletal muscle during lipid overfeeding in healthy men. Design/Participants/Intervention: Thirty-nine healthy volunteers were overfed ...

Endocrine Soc2014