Publications associées à Dietary Fiber Is Essential to Maintain Intestinal Size, L-Cell Secretion, and Intestinal Integrity in Mice

Characterization of the gut-bone marrow axis through bile acid signaling

Communication between the intestine and other organs such as the lungs, brain or bones is mediated by several metabolites, like short-chain fatty acids or bile acids, that relay information about nutritional and microbiota status. Bile acids are endogenous ...

EPFL2024

Innovative Biomarkers for Obesity and Type 1 Diabetes Based on Bifidobacterium and Metabolomic Profiling

Stephan Morgenthaler, Paul Refinetti, Mariya Yuryevna Skvortsova

The role of Bifidobacterium species and microbial metabolites such as short-chain fatty acids (SCFAs) and human milk oligosaccharides in controlling intestinal inflammation and the pathogenesis of obesity and type 1 diabetes (T1D) has been largely studied ...

MDPI2024

Microfluidic systems for infectious disease diagnostics

Thomas Lehnert, Martinus Gijs

Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, i ...

Royal Soc Chemistry2024

Quench Detection and Temperature Measurement With Fiber Optic Sensors

Davide Uglietti, Kamil Sedlák, Christoph Müller

For the EU DEMO conductor testing, a temperature sensor based on Fiber Bragg Grating (FBG) optical fiber is studied at the EPFL Swiss Plasma Center. The SULTAN test facility has been upgraded to use fiber optic sensor for temperature monitoring and quench ...

IEEE Institute of Electrical and Electronics Engineers2024

Accessible and highly observable gastrointestinal organoid model systems for studying host-pathogen interactions

Moritz Hofer

Traditional cell cultures have long been fundamental to biological research, offering an alternative to animal models burdened by ethical constraints and procedural intricacies, often lacking relevance to human physiology and disease. Moreover, their inabi ...

EPFL2024

Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi

Anders Meibom, Stéphane Laurent Escrig

Diverse bacteria can colonize the animal gut using dietary nutrients or by engaging in microbial crossfeeding interactions. Less is known about the role of host-derived nutrients in enabling gut bacterial colonization. Here we examined metabolic interactio ...

Nature Portfolio2024

Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease

Yogesh Singh Chauhan, Hilal Lashuel, Florian Lang, Joan Romani Aumedes, Yogesh Singh

Background Braak's hypothesis states that sporadic Parkinson's disease (PD) follows a specific progression of pathology from the peripheral to the central nervous system, and this progression can be monitored by detecting the accumulation of alpha-Synuclei ...

BMC2023

Identifying genetic and dietary modulators of metabolic disorders using systems genetics

Xiaoxu Li

Long-term consumption of lipid-rich foods can contribute to common metabolic diseases and systemic low-grade inflammation. However, dietary responses and the development of non-communicable diseases are shaped by genetic factors and gene-by-environment int ...

EPFL2023

Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine

Farzad Rezaeianaran

In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...

EPFL2023

High-resolution imaging and analysis of the intestinal bacterial load of Caenorhabditis elegans during early adulthood

Martinus Gijs, Farzad Rezaeianaran

We study the presence within the worm Caenorhabditis elegans (C. elegans) of a fluorescent strain of the worm's bacterial food (Escherichia coli (E. coli) OP50) during early adulthood. Use of a microfluidic chip based on a thin glass coverslip substrate al ...

ROYAL SOC CHEMISTRY2023

Bioengineering human mini-intestines with in vivo-like complexity and function

Olga Mitrofanova

Biological research heavily relies on the use of animal models, which has made it difficult to answer specific questions about human biology and disease. However, with the advent of human organoids - miniature versions of tissues generated in 3D human stem ...

EPFL2023

Sporadic type VI secretion in seventh pandemic Vibrio cholerae

Melanie Blokesch, Sandrine Stutzmann, Lisa Christina Metzger, Natalia Carolina Drebes Dorr, Sandrine Natacha Isaac, Nicolas René Chiaruttini, Alexis Joseph Proutière, Loriane Bader

Vibrio cholerae is a pathogen that causes disease in millions of people every year by colonizing the small intestine and then secreting the potent cholera toxin. How the pathogen overcomes the colonization barrier created by the host’s natural microbiota i ...

2023

The Effects of Spatial Organization and Fluid Flow on the Social Interactions in Microbial Communities

Jeremy Pak Hei Wong

Bacteria often engage in social interactions with neighbouring bacteria. Ecosystems which are subjected to social interactions have been widely studied in well mixed settings such as test tubes, allowing us to identify the cellular components contributing ...

EPFL2023

Methanobrevibacter smithii ( m. smithii) for use as a biomarker and in the diagnosis and the treatment of disorders associated with aberrant microbiota and/or archaea-deficiency

Duncan-Bruce Sutherland

The present invention generally relates to the field of intestinal health, disorders associated with aberrant microbiota and/or archaea-deficiency, and early-life immune system development. The present invention more specifically relates to M. smithii and ...

2023

Process development of pre-impregnated hybrid thin-ply composites

Alexios Argyropoulos

Thin-ply composites, obtained with recently developed fiber spreading techniques, rapidly gained industrial interest because they offer a large composite design freedom, and lead to a composite tensile strength that is close to that of the individual fiber ...

EPFL2023

Apelin-driven endothelial cell migration sustains intestinal progenitor cells and tumor growth

Mauro Delorenzi, Stefanie Siegert, Pascale Anderle Pedone, Nadine Fournier, Sina Nassiri, François Renevey, Michele De Palma, Mario Leonardo Squadrito, Simone Ragusa

Stem and progenitor cells residing in the intestinal crypts drive the majority of colorectal cancers (CRCs), yet vascular contribution to this niche remains largely unexplored. Vascular endothelial growth factor A (VEGFA) is a key driver of physiological a ...

Springernature2022

Multidisciplinary Investigation of the Gut-Brain Ecosystem in a Model of Alzheimer's Disease

Arielle Louise Planchette

The search for an understanding of the causal elements that lead to neurodegenerative diseases has motivated researchers for decades. Today, Alzheimer's disease is the most prevalent form of dementia and affects approximately fifty million people worldwide ...

EPFL2022

Fiber Buckling in Confined Viscous Flows: An Absolute Instability Described by the Linear Ginzburg-Landau Equation

François Gallaire, Jean Cappello

We explore the dynamics of a flexible fiber transported by a viscous flow in a Hele-Shaw cell of height comparable to the fiber height. We show that long fibers aligned with the flow experience a buckling instability. Competition between viscous and elasti ...

AMER PHYSICAL SOC2022

IL-17 controls central nervous system autoimmunity through the intestinal microbiome

Sandrine Natacha Isaac

Interleukin-17A- (IL-17A) and IL-17F-producing CD4(+) T helper cells (T(H)17 cells) are implicated in the development of chronic inflammatory diseases, such as multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). T-H 17 ...

2021

High-content, targeted RNA-seq screening in organoids for drug discovery in colorectal cancer

Jörg Hülsken, Paloma Ordonez Moran, Maxim Norkin

Organoids allow the recapitulation of intestinal homeostasis and cancerogenesis in vitro; however, RNA sequencing (RNA-seq)-based methods for drug screens are missing. We develop targeted organoid sequencing (TORNADO-seq), a high-throughput, high-content d ...

2021