Recherche

Afficher tous les résultats pour

Accueil

Personne

Yury Tsybin

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (75)

Page 3 sur 4

In-Spray Supercharging of Peptides and Proteins in Electrospray Ionization Mass Spectrometry

Krzysztof Marek Piech, Hubert Girault, Yury Tsybin, Luca Fornelli, Yu Lu, Sasa Miladinovic

Enhanced charging, or supercharging, of analytes in electrospray ionization mass spectrometry (ESI MS) facilitates high resolution MS by reducing an ion mass-to-charge (m/z) ratio, increasing tandem mass spectrometry (MS/MS) efficiency. ESI MS superchargin ...

2012

Multiplexed Middle-Down Mass Spectrometry as a Method for Revealing Light and Heavy Chain Connectivity in a Monoclonal Antibody

Yury Tsybin, Natalia Gasilova, Laure Menin, Anton Kozhinov, Luca Fornelli, Konstantin Nagornov, Kristina Srzentic, Daniel Ayoub

Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) or middle-down (MD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, ...

AMER CHEMICAL SOC2018

Repeatability and reproducibility of product ion abundances in electron capture dissociation mass spectrometry of peptides

Yury Tsybin, Aleksey Vorobyev, Hisham Ben Hamidane

Site-specific reproducibility and repeatability of electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) are of fundamental importance for product ion abundance (PIA)-based peptide and protein struct ...

2011

Composition of hydrofullerene mixtures produced by C60 reaction with hydrogen gas revealed by high-resolution mass spectrometry

Andreas Züttel, Yury Tsybin, Philippe Mauron

2005

Principles of electron capture and transfer dissociation mass spectrometry applied to peptide and protein structure analysis

Yury Tsybin, Matthew Wodrich, Konstantin Zhurov, Luca Fornelli, Unige Anna Laskay

This tutorial review describes the principles and practices of electron capture and transfer dissociation (ECD/ETD or ExD) mass spectrometry (MS) employed for peptide and protein structure analysis. ExD MS relies on interactions between gas phase peptide o ...

Royal Society of Chemistry2013

Fragmentation methods on the balance: unambiguous top–down mass spectrometric characterization of oxaliplatin–ubiquitin binding sites

Paul Joseph Dyson, Yury Tsybin, Christian Hartinger

The interaction between oxaliplatin and the model protein ubiquitin (Ub) was investigated in a top-down approach by means of high-resolution electrospray ionization mass spectrometry (ESI-MS) using diverse tandem mass spectrometric (MS/MS) techniques, incl ...

Springer-Verlag2012

Advantages of Extended Bottom-Up Proteomics Using Sap9 for Analysis of Monoclonal Antibodies

Yury Tsybin, Luca Fornelli, Kristina Srzentic, Unige Anna Laskay, Daniel Ayoub

Despite the recent advances in structural analysis of monoclonal antibodies with bottom-up, middle-down, and top-down mass spectrometry (MS), further improvements in analysis accuracy, depth, and speed are needed. The remaining challenges include quantitat ...

Amer Chemical Soc2014

Top-down analysis of 30–80 kDa proteins by electron transfer dissociation time-of-flight mass spectrometry

Yury Tsybin, Luca Fornelli

Electron transfer dissociation (ETD)-based top-down mass spectrometry (MS) is the method of choice for in-depth structure characterization of large peptides, small- and medium-sized proteins, and non-covalent protein complexes. Here, we describe the perfor ...

Springer Berlin Heidelberg2013

From High- to Super-resolution Mass Spectrometry

Yury Tsybin

High-resolution mass spectrometry (MS) is indispensable for the molecular-level analysis of biological and environmental samples with great intra- and inter-molecular complexity. Here, we summarize developments in Fourier transform mass spectrometry (FTMS) ...

Schweizerische Chemische Gesellschaft2014

High-Resolution and Tandem Mass Spectrometry – the Indispensable Tools of the XXI Century

Yury Tsybin, Anton Kozhinov, Luca Fornelli, Aleksey Vorobyev, Sasa Miladinovic

Mass spectrometry-based qualitative and quantitative (bio)molecular analysis is a corner stone in the state-of-the-art pipelines in systems biology and environmental sciences. High-resolution and efficient tandem mass spectrometry methods and techniques ar ...

Schweizerische Chemische Gesellschaft2011

Observation of ion coalescence in Orbitrap Fourier transform mass spectrometry

Yury Tsybin, Luca Fornelli

RATIONALE Similar to other mass spectrometric technologies based on ion trapping in a spatially restricted area, the performance of Orbitrap Fourier transform mass spectrometry (FTMS) is affected by the interaction between the trapped ion clouds. One of th ...

Wiley-Blackwell2012

Identification of hemoglobin variants by top-down mass spectrometry using selected diagnostic product ions

Yury Tsybin

Hemoglobin disorder diagnosis is a complex procedure combining several analytical steps. Due to the lack of specificity of the currently used protein analysis methods, the identification of uncommon hemoglobin variants (proteoforms) can become a hard task ...

Springer Heidelberg2015

Ping-Pong Protons: How Hydrogen Bonding Networks Facilitate Heterolytic Bond Cleavages in Peptide Radical Cations

Anne-Clémence Corminboeuf, Yury Tsybin, Matthew Wodrich, Konstantin Zhurov

Electron capture and electron transfer dissociation (ECD/ETD) tandem mass spectrometry (MS/MS) are commonly employed techniques for biomolecular analysis. The ECD/ETD process predominately cleaves N-C-alpha peptide backbone bonds, leading to primary sequen ...

Amer Chemical Soc2014

Coronene Fusion by Heat Treatment: Road to Nanographenes

Yury Tsybin, Klaus Leifer

The reactions of coronene dehydrogenation and fusion upon heat treatment in the temperature range of 500-700 degrees C were studied using XRD, TEM, Raman, IR, and NEXAFS spectroscopy. The formation of a coronene dimer (dicoronylene) was observed at tempera ...

American Chemical Society2011

Thermal Decomposition of C60H18

Yury Tsybin

Products of thermal dehydrogenation of C60H18 (which mainly occurs at 450-600 degrees C) were studied by XRD, Raman, IR and mass spectrometry. IR spectra indicate that dehydrogenation resulted in partial recovery of pristine C-60. XRD data indicate that th ...

2009

Gold finger formation studied by high-resolution mass spectrometry and in silico methods

Yury Tsybin, Angela Casini, Unige Anna Laskay

High-resolution mass spectrometry and quantum mechanics/molecular mechanics studies were employed for characterizing the formation of two gold finger (GF) domains from the reaction of zinc fingers (ZF) with gold complexes. The influence of both the gold ox ...

Royal Society of Chemistry2015

Filter Diagonalization Method-Based Mass Spectrometry for Molecular and Macromolecular Structure Analysis

Yury Tsybin, Anton Kozhinov

Molecular and macromolecular structure analysis by high resolution and accurate mass spectrometry (MS) is indispensable for a number of fundamental and applied research areas, including health and energy domains. Comprehensive structure analysis of molecul ...

2012

Comparative Dissociation of Peptide Polyanions by Electron Impact and Photo-Induced Electron Detachment

Yury Tsybin, Aleksey Vorobyev

We compare product-ion mass spectra produced by electron detachment dissociation (EDD) and electron photodetachment dissociation (EPD) of multi-deprotonated peptides on a Fourier transform and a linear ion trap mass spectrometer, respectively. Both methods ...

2010

Analysis of Intact Monoclonal Antibody IgG1 by Electron Transfer Dissociation Orbitrap FTMS

Yury Tsybin, Luca Fornelli

The primary structural information of proteins employed as biotherapeutics is essential if one wishes to understand their structure-function relationship, as well as in the rational design of new therapeutics and for quality control. Given both the large s ...

Amer Soc Biochemistry Molecular Biology Inc2012

Identification of Single and Double Sites of Phosphorylation by ECD FT-ICR/MS in Peptides Related to the Phosphorylation Site Domain of the Myristoylated Alanine-rich C Kinase Protein

Yury Tsybin

2007