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Yury Tsybin

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Publications associées (75)

Heterolytic N-Calpha Bond Cleavage in Electron Capture and Transfer Dissociation of Peptide Cations

Hisham Ben Hamidane, Yury Tsybin, Anne-Clémence Corminboeuf, Konstantin Zhurov, Matthew Wodrich, Aleksey Vorobyev

Mass spectrometry techniques employing electron capture and electron transfer dissociation represent powerful approaches for the analysis of biological samples. Despite routine employment in analytical fields, the underlying physical processes dictating pe ...

Amer Chemical Soc2012

On the utility of predictive chromatography to complement mass spectrometry based intact protein identification

Hisham Ben Hamidane, Yury Tsybin

The amino acid sequence determines the individual protein three-dimensional structure and its functioning in an organism. Therefore, "reading" a protein sequence and determining its changes due to mutations or post-translational modifications is one of the ...

Springer-Verlag2012

On the Utility of Isotopic Fine Structure Mass Spectrometry in Protein Identification

Yury Tsybin, Anton Kozhinov, Sasa Miladinovic

Modern mass spectrometry (MS)-based protein identification and characterization relies upon accurate mass measurements of the C-13 isotopic distributions of the enzymatically produced peptides. Interestingly, obtaining peptide elemental composition informa ...

2012

Filter Diagonalization Method-Based Mass Spectrometry for Molecular and Macromolecular Structure Analysis

Yury Tsybin, Anton Kozhinov

Molecular and macromolecular structure analysis by high resolution and accurate mass spectrometry (MS) is indispensable for a number of fundamental and applied research areas, including health and energy domains. Comprehensive structure analysis of molecul ...

2012

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, Simone Schmitt, Yury Tsybin, Luca Fornelli, Julien Parra

In this study, a recombinant monoclonal IgG antibody was produced by transient gene expression (TGE) in suspension-adapted HEK-293E cells. The objective of the study was to determine the variation in recombinant IgG yield and glycosylation in ten independe ...

Elsevier2012

In-Spray Supercharging of Peptides and Proteins in Electrospray Ionization Mass Spectrometry

Hubert Girault, Yury Tsybin, Yu Lu, Luca Fornelli, Sasa Miladinovic, Krzysztof Marek Piech

Enhanced charging, or supercharging, of analytes in electrospray ionization mass spectrometry (ESI MS) facilitates high resolution MS by reducing an ion mass-to-charge (m/z) ratio, increasing tandem mass spectrometry (MS/MS) efficiency. ESI MS superchargin ...

2012

Electron Transfer Dissociation Mass Spectrometry of Hemoglobin on Clinical Samples

Yury Tsybin

A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whol ...

Springer2012

Statistical Analysis of Ion Mobility Spectrometry. I. Unbiased and Guided Replica-Exchange Molecular Dynamics

Yury Tsybin

Achieving (bio) macromolecular structural assignment from the interpretation of ion mobility spectrometry (IMS) experiments requires successful comparison with computer modeling. Replica-exchange molecular dynamics simulations with suitable force fields no ...

2012

UV radiation induces genome-mediated, site-specific cleavage in viral protein

Hisham Ben Hamidane, Anne Thérèse Sigstam, Michele Cascella, Tamar Kohn, Yury Tsybin, Laure Menin, Krista Rule Wigginton

Much research has been dedicated to understanding the molecular basis of UV damage to biomolecules, yet many questions remain regarding the specific pathways involved. Here we describe a genome-mediated mechanism that causes sitespecific virus protein clea ...

2012

High-Resolution and Tandem Mass Spectrometry – the Indispensable Tools of the XXI Century

Yury Tsybin, Aleksey Vorobyev, Luca Fornelli, Anton Kozhinov, Sasa Miladinovic

Mass spectrometry-based qualitative and quantitative (bio)molecular analysis is a corner stone in the state-of-the-art pipelines in systems biology and environmental sciences. High-resolution and efficient tandem mass spectrometry methods and techniques ar ...

Schweizerische Chemische Gesellschaft2011

Repeatability and reproducibility of product ion abundances in electron capture dissociation mass spectrometry of peptides

Hisham Ben Hamidane, Yury Tsybin, Aleksey Vorobyev

Site-specific reproducibility and repeatability of electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) are of fundamental importance for product ion abundance (PIA)-based peptide and protein struct ...

2011

Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry

Florian Maria Wurm, Sophie Nallet, Yury Tsybin, Luca Fornelli, Julien Parra

Improving qualitative and quantitative characterization of monoclonal antibodies is essential, because of their increasing popularity as therapeutic drug targets. Electron transfer dissociation (ETD)-based top-down mass spectrometry (MS) is the method of c ...

2011

Tissue Transglutaminase-mediated Glutamine Deamidation of beta-Amyloid Peptide Increases Peptide Solubility, Whereas Enzymatic Cross-linking and Peptide Fragmentation May Serve as Molecular Triggers for Rapid Peptide Aggregation

Horst Vogel, Enrico Condemi, Adrian Schmid, Marc Moniatte, Diego Chiappe, Yury Tsybin

Tissue transglutaminase (TGase) has been implicated in a number of cellular processes and disease states, where the enzymatic actions of TGase may serve in both, cell survival and apoptosis. To date, the precise functional properties of TGase in cell survi ...

American Society for Biochemistry and Molecular Biology2011

Structural Preferences of Gas-Phase M2TMP Monomers upon Sequence Variations

Rodolphe Antoine, Hisham Ben Hamidane, Yury Tsybin

The conformations of a number of M2TMP-(22-46) sequence variants have been investigated using ion mobility spectrometry (IMS). Substantial conformational changes were evidenced by IMS upon the variation of a single amino acid in the peptide sequence, with ...

2011

Towards data acquisition throughput increase in Fourier transform mass spectrometry of proteins using double frequency measurements

Yury Tsybin, Aleksey Vorobyev

Combined with high and ultra high pressure liquid chromatography, mass spectrometry-based peptide and protein structure analysis requires high resolution and mass accuracy achievable within short ion detection time. Although Fourier transform ion cyclotron ...

Elsevier2011

Reaction of C60 with Hydrogen Gas: In Situ Monitoring and Pathways

Yury Tsybin

Kinetics and pathways of C60 reaction with hydrogen gas were studied in a broad temperature interval and over extended periods of time. Specifically, hydrogenation was monitored in situ at high temperature and high hydrogen pressure conditions using the gr ...

American Chemical Society2011

Deamidation and Transamidation of Substance P by Tissue Transglutaminase Revealed by Electron-Capture Dissociation Fourier Transform Mass Spectrometry (Chem. Eur. J. 2/2011)

Horst Vogel, Luigino Grasso, Adrian Schmid, Yury Tsybin, Luca Fornelli

Tissue transglutaminase (tTGase) catalyzes both deamidation and transamidation of peptides and proteins by using a peptidyl glutamine as primary substrate. A precise consensus sequence for the enzyme is unknown and the ratio between deamidated and transami ...

Wiley-Blackwell2011

Coronene Fusion by Heat Treatment: Road to Nanographenes

Klaus Leifer, Yury Tsybin

The reactions of coronene dehydrogenation and fusion upon heat treatment in the temperature range of 500-700 degrees C were studied using XRD, TEM, Raman, IR, and NEXAFS spectroscopy. The formation of a coronene dimer (dicoronylene) was observed at tempera ...

American Chemical Society2011

Comparative Dissociation of Peptide Polyanions by Electron Impact and Photo-Induced Electron Detachment

Rodolphe Antoine, Yury Tsybin, Aleksey Vorobyev

We compare product-ion mass spectra produced by electron detachment dissociation (EDD) and electron photodetachment dissociation (EPD) of multi-deprotonated peptides on a Fourier transform and a linear ion trap mass spectrometer, respectively. Both methods ...

2010

Ruthenium versus platinum: interactions of anticancer metallodrugs with duplex oligonucleotides characterised by electrospray ionisation mass spectrometry

Paul Joseph Dyson, Yury Tsybin, Christian Hartinger, Michael Groessl

The binding of the ruthenium-based anticancer drug candidates KP1019, NAMI-A and RAPTA-T towards different double-stranded oligonucleotides was probed by electrospray ionisation mass spectrometry and compared with that of the widely used platinum-based che ...

Springer-Verlag2010

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