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Harm-Anton Klok

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Publications associées (195)

Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells

Theo Lasser, Harm-Anton Klok, Zuzana Kadlecova

An optical method is presented that allows the measurement of the triplet lifetime of a fluorescent molecule. This is a characteristic specific to each fluorophore. Based on differences in triplet lifetimes of two fluorescent species (autofluorescence vers ...

Optical Soc Amer2012

Polyvalent Side Chain Peptide-Synthetic Polymer Conjugates as HIV-1 Entry Inhibitors

Harm-Anton Klok, Maarten Danial

This report describes the synthesis and properties of a series of polyvalent side chain peptide synthetic polymer conjugates designed to block the CD4 binding site on gp120 and inhibit HIV-1 entry into a host cell. The peptide sequences in the conjugates a ...

American Chemical Society2012

Voltammetric Detection of Hg2+ Using Peptide-Functionalized Polymer Brushes

Harm-Anton Klok, Maarten Danial

Polymer brushes grafted by surface-initiated atom transfer radical polymerization (SI-ATRP) from the surface of Ir-based microelectrode arrays are explored as a platform for the fabrication of sensory coatings for the voltammetric detection of Hg2+. The po ...

Csiro Publishing2012

Functional, Hyperbranched Polyesters Via Baylis-Hillman Polymerization

Harm-Anton Klok, Sanhao Ji, Frederik Roman Wurm

Hyperbranched polyesters are among the most common hyperbranched polymers. One of the interesting features of hyperbranched polyesters is that they contain unreacted hydroxyl and carboxylic acid groups at the linear and terminal structural units, which can ...

2012

Size- and Coating-Dependent Uptake of Polymer-Coated Gold Nanoparticles in Primary Human Dermal Microvascular Endothelial Cells

Harm-Anton Klok, Matthew Gibson

A library-orientated approach is used to gain understanding of the interactions of well-defined nanoparticles with primary human endothelial cells, which are a key component of the vasculature. Fifteen sequentially modified gold nanoparticles (AuNPs) based ...

American Chemical Society2012

Osteoconductive surfaces generated from peptide functionalized poly(2-hydroxyethyl methacrylate-co-2-(methacryloyloxy)ethyl phosphate) brushes

Harm-Anton Klok, Dusko Paripovic

Modification of bone implant materials with biofunctional surfaces that facilitate osteoblast adhesion and promote matrix mineralization is an attractive strategy to improve osseointegration. This manuscript discusses the preparation and properties of RGD ...

Royal Society of Chemistry2012

Squaric Acid Mediated Synthesis and Biological Activity of a Library of Linear and Hyperbranched Poly(Glycerol)-Protein Conjugates

Harm-Anton Klok, Frederik Roman Wurm

Polymer-protein conjugates generated from side chain functional synthetic polymers are attractive because they can be easily further modified with, for example, labeling groups or targeting ligands. The residue specific modification of proteins with side c ...

American Chemical Society2012

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker, Harm-Anton Klok, Zuzana Kadlecova

Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI Max) regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI ...

Wiley-Blackwell2012

Diagnostic and Sensory Polymer Brushes

Raphaël Barbey, Harm-Anton Klok

2011

Conformational consequences of cooperative binding of a coiled-coil peptide motif to poly(N-(2-hydroxypropyl) methacrylamide) HPMA copolymers

Harm-Anton Klok

Small-angle neutron scattering and pulsed-gradient spin-echo NMR have been used to examine the solution conformation of a series of water soluble poly(N-(2-hydroxypropyl) methacrylamide) P(HPMA) co-polymer drug delivery vehicles incorporating a coiled-coil ...

Elsevier2011

Polymers Interfacing with Biology

Harm-Anton Klok, Frederik Roman Wurm

The development of various controlled radical polymerization techniques as well as site- and residue specific strategies to modify peptides/proteins with synthetic polymers have made polymer chemistry a powerful tool to address materials problems at the bi ...

2011

Exploring the Scope of the Baylis-Hillman Reaction for the Synthesis of Side-Chain Functional Polyesters

Harm-Anton Klok, Sanhao Ji

The BaylisHillman reaction provides a protective-group-free strategy to heterobifunctional polyesters, which can be further modified via various post-polymerization modification reactions. This contribution explores the BaylisHillman polymerization of 1,3- ...

2011

Sequentially Modified, Polymer-Stabilized Gold Nanoparticle Libraries: Convergent Synthesis and Aggregation Behavior

Harm-Anton Klok, Maarten Danial, Matthew Gibson

This manuscript describes a versatile, yet experimentally facile, method for producing libraries of polymer coated (core-shell type) gold nanoparticles. The synthetic principle relies on two, sequential postmodification reactions, which ensures homogeneity ...

2011

Cell Uptake and Trafficking Behavior of Non-covalent, Coiled-coil Based Polymer-Drug Conjugates

Harm-Anton Klok

This paper reports on the cell uptake and trafficking properties of a series of non-covalent polymer-drug conjugates. These nanomedicines are composed of a poly(N-(2-hydroxypropyl)methacrylamide) backbone functionalized with multiple copies of a drug. The ...

Wiley-Blackwell2011

Neutron Reflectivity Study on the Postpolymerization Modification of Poly(2-hydroxyethyl methacrylate) Brushes

Harm-Anton Klok, Juan Pablo Hinestrosa Salazar

Postpolymerization modification reactions are widely employed to prepare functional polymer brushes. Relatively little is known, however, about the distribution of functional groups in such postmodified brushes. Using neutron reflectometry and UV-vis spect ...

American Chemical Society2011

Improving the Stability in Aqueous Media of Polymer Brushes Grafted from Silicon Oxide Substrates by Surface-Initiated Atom Transfer Radical Polymerization

Harm-Anton Klok, Dusko Paripovic

Hydrophilic polymer brushes grown via surface-initiated ATRP from silicon oxide surfaces are susceptible to detachment via hydrolytic cleavage of the anchoring siloxane bond. This paper investigates the influence of the structure of the ATRP initiator on t ...

2011

Side-Chain Peptide-Synthetic Polymer Conjugates via Tandem "Ester-Amide/Thiol-Ene" Post-Polymerization Modification of Poly(pentafluorophenyl methacrylate) Obtained Using ATRP

Harm-Anton Klok, Maarten Danial, Matthew Gibson

Herein the concept of tandem postpolymerization modification as a versatile route to synthesize well-defined, highly functionalized polymers is introduced. Poly(pentafluorophenyl methacrylate) obtained by atom transfer radical polymerization was first modi ...

American Chemical Society2011

Aqueous solution self-assembly of polystyrene-b-poly(L-lysine) diblock oligomers

Harm-Anton Klok, Harald Nuhn

This paper reports on the dilute, aqueous solution self-assembly of two asymmetric polystyrene-b-poly(L-lysine) (PSnPKm) diblock oligomers with PS and PK number-average degrees of polymerization of eight, respectively, 40 and 60. The self-assembly of these ...

2011

Tuning the pH Sensitivity of Poly(methacrylic acid) Brushes

Harm-Anton Klok

The pH-induced swelling and collapse of surface-tethered, weak polyelectrolyte brushes is of interest for the development of actuators or to allow pH controlled transport or adsorption. This contribution discusses results of an extensive series of quartz c ...

2011

Arginine-specific protein modification using alpha-oxo-aldehyde functional polymers prepared by atom transfer radical polymerization

Harm-Anton Klok, Maxime Ayer, Marc Gauthier, Justyna Kowal, Frederik Roman Wurm

The residue-specific modification of peptides and proteins is a powerful strategy for preparing biomolecular-synthetic polymer conjugates with advanced properties. This manuscript aims at expanding the present toolbox of residue-selective protein modificat ...

2011

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