Publications de Luc Reymond | EPFL Graph Search

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During a previous, exploratory project in our group, it was observed that duplexes formed by oligonucleotides modified with polar 2'-O-substituents were more tolerant towards the addition of 1,2-dimethoxyethane (DME) than RNA and DNA duplexes [1]. Inspired ...

Gene delivery using vector or viral-based methods is often limited by technical and safety barriers. A promising alternative that circumvents these shortcomings is the direct delivery of proteins into cells. Here we introduce a non-viral, ligand-mediated p ...

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

The study of membranes is at a turning point. New theories about membrane structure and function have recently been proposed, however, new technologies, combining chemical, physical, and biochemical approaches are necessary to test these hypotheses. In par ...

Single-molecule Forster resonance energy transfer (FRET) and photoinduced electron transfer (PET) have developed into versatile and complementary methods for probing distances and dynamics in biomolecules. Here we show that the two methods can be combined ...

Microtubules, a critical component of the cytoskeleton, carry post-translational modifications (PTMs) that are important for the regulation of key cellular processes. Long-lived microtubules, in neurons particularly, exhibit both detyrosination of a-tubuli ...

Molecular recognition events between proteins drive biological processes in living systems1. However, higher levels of mechanistic regulation have emerged, in which protein-protein interactions are conditioned to small molecules2, 3, 4-5. Despite recent ad ...

Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegen ...

We describe the computational design of proteins that bind the potent analgesic fentanyl. Our approach employs a fast docking algorithm to find shape complementary ligand placement in protein scaffolds, followed by design of the surrounding residues to opt ...

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over ...

Cysteine cathepsins are a family of proteases that are relevant therapeutic targets for the treatment of different cancers and other diseases. However, no clinically approved drugs for these proteins exist, as their systemic inhibition can induce deleterio ...

The Fox-1 protein regulates alternative splicing of tissue-specific exons by binding to GCAUG elements. Here, we report the soln. structure of the Fox-1 RNA binding domain (RBD) in complex with UGCAUGU. The last three nucleotides, UGU, are recognized in a ...

We report an optimized synthesis of all canonical 2'-O-TOM protected ribonucleoside phosphoramidites and solid supports containing [13C5]-labeled ribose moieties, their sequence-specific introduction into very short RNA sequences and their use for the stru ...

Rhodamines have been continuously optimized in brightness, biocompatibility, and color to fulfill the demands of modern bioimaging. However, the problem of phototoxicity caused by the excited fluorophore under long-term illumination has been largely neglec ...

While gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter, suitable tools to measure its concentration in living cells with high spatiotemporal resolution are missing. Herein, we describe the first ratiometric fluorescent sensor for GABA ...

The centrosome functions as the main microtubule-organizing center of animal cells and is crucial for several fundamental cellular processes [1]. Abnormalities in centrosome number and composition correlate with tumor progression [2, 3] and other diseases ...

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynt ...

The present invention relates to compounds, compositions for use and methods for the treatment of a STING associated disease, condition, or disorder. ...

At its most fundamental level, touch sensation requires the translation of mechanical energy into mechanosensitive ion channel opening, thereby generating electro-chemical signals. Our understanding of this process, especially how the cytoskeleton influenc ...

Luc Reymond

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Synthesis and evaluation of 2'-O-substituted oligoribonucleotide analogues with new properties

A ligand-based system for receptor-specific delivery of proteins

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall

Chemical Biology Approaches to Membrane Homeostasis and Function

Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

Tubulin engineering by semi-synthesis reveals that polyglutamylation directs detyrosination

Targeting protein-ligand neosurfaces with a generalizable deep learning tool

Fluorescent fatty acid conjugates for live cell imaging of peroxisomes

Computational design of environmental sensors for the potent opioid fentanyl

Visualizing Biochemical Activities in Living Cells through Chemistry

Antibody-peptide conjugates deliver covalent inhibitors blocking oncogenic cathepsins

Molecular basis of RNA recognition by the human alternative splicing factor Fox-1

Short, synthetic and selectively 13C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes

Gentle Rhodamines for Live-Cell Fluorescence Microscopy

A Fluorescent Sensor for GABA and Synthetic GABA(B) Receptor Ligands

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Sting inhibitors

Acetylated tubulin is essential for touch sensation in mice