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Luc Reymond

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Publications associées (46)

Using Laser-Induced Rydberg Spectroscopy diagnostic for direct measurements of the local electric field in the edge region of NSTX/NSTX-U: Modeling

Luc Reymond

We discuss a novel diagnostic allowing direct measurements of the local electric field in the edge region of NSTX/NSTX-U. This laser based diagnostic's principle consists of depleting the naturally populated n = 3 level to a Rydberg state-sensitive to elec ...

2018

Computational design of environmental sensors for the potent opioid fentanyl

Kai Johnsson, Luc Reymond

We describe the computational design of proteins that bind the potent analgesic fentanyl. Our approach employs a fast docking algorithm to find shape complementary ligand placement in protein scaffolds, followed by design of the surrounding residues to opt ...

Elife Sciences Publications Ltd2017

Luciferases with tunable emission wavelengths

Kai Johnsson, Luc Reymond, Olivier Vincent Sallin, Alberto Schena, Rudolf Griss, Qiuliyang Yu, Lin Xue, Julien Hiblot, Nicholas Clarke Hill, Marina Daniela Beatrice Sabbadini

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. These tunable luciferases are generated by inserting the small luciferase NanoLuc into the structures of the self-labeling proteins SNAP-tag or HaloT ...

Wiley-Blackwell2017

Acetylated tubulin is essential for touch sensation in mice

Kai Johnsson, Luc Reymond, Antonino Asaro

At its most fundamental level, touch sensation requires the translation of mechanical energy into mechanosensitive ion channel opening, thereby generating electro-chemical signals. Our understanding of this process, especially how the cytoskeleton influenc ...

Elife Sciences Publications Ltd2016

Sensors, methods and kits for detecting nicotinamide adenine dinucleotides

Kai Johnsson, Luc Reymond, Olivier Vincent Sallin

The invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence or luminescence-based detection o ...

2016

Fluorogenic Probes for Multicolor Imaging in Living Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius, Olivier Vincent Sallin, Keitaro Umezawa

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag ar ...

American Chemical Society2016

Genetic targeting of chemical indicators in vivo

Kai Johnsson, Luc Reymond, Antonino Asaro

Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labe ...

Nature Publishing Group2015

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius, Alberto Schena

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...

Nature Research2015

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Luc Reymond, Grazvydas Lukinavicius, Suliana Manley, Aleksandr Benke, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red

Rudolf Hovius, Kai Johnsson, Luc Reymond, Howard Riezman, Efthymia Prifti

A fluorogenic probe for plasma membrane proteins based on the dye Nile Red and SNAP-tag is introduced. It takes advantage of Nile Red, a solvatochromic molecule highly fluorescent in an apolar environment, such as cellular membranes, but almost dark in a p ...

Amer Chemical Soc2014

Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

Luc Reymond

Single-molecule Forster resonance energy transfer (FRET) and photoinduced electron transfer (PET) have developed into versatile and complementary methods for probing distances and dynamics in biomolecules. Here we show that the two methods can be combined ...

Amer Chemical Soc2013

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

Kai Johnsson, Luc Reymond, Pierre Gönczy, Grazvydas Lukinavicius, Keitaro Umezawa

The centrosome functions as the main microtubule-organizing center of animal cells and is crucial for several fundamental cellular processes [1]. Abnormalities in centrosome number and composition correlate with tumor progression [2, 3] and other diseases ...

Elsevier2013

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Bentham Science Publ Ltd2013

Certain cancer and RASopathy associated mutations affect Ras nanoclustering

Luc Reymond, Camilo Guzman

Wiley-Blackwell2013

A Fluorescent Sensor for GABA and Synthetic GABA(B) Receptor Ligands

Kai Johnsson, Luc Reymond, Anastasiya Masharina, Keitaro Umezawa

While gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter, suitable tools to measure its concentration in living cells with high spatiotemporal resolution are missing. Herein, we describe the first ratiometric fluorescent sensor for GABA ...

American Chemical Society2012

A Semisynthetic Fluorescent Sensor Protein for Glutamate

Kai Johnsson, Luc Reymond, Matthias Albert Brun, Kui Thong Tan, Rudolf Griss

We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor di ...

2012

Visualizing Biochemical Activities in Living Cells through Chemistry

Kai Johnsson, Luc Reymond, Matthias Albert Brun, Karolina Bojkowska, Birgit Mollwitz, Grazvydas Lukinavicius, Anastasiya Masharina, Alberto Schena, Keitaro Umezawa, Rudolf Griss

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over ...

2011

Chemical Biology Approaches to Membrane Homeostasis and Function

Gerardo Turcatti, Kai Johnsson, Luc Reymond, Christian Heinis, Stefan Matile, Howard Riezman

The study of membranes is at a turning point. New theories about membrane structure and function have recently been proposed, however, new technologies, combining chemical, physical, and biochemical approaches are necessary to test these hypotheses. In par ...

2011

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Horst Vogel, Kai Johnsson, Joachim Piguet, Luc Reymond, Matthias Albert Brun, Kui Thong Tan, Ruud Peters, Rudolf Griss

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynt ...

2011

Conformational dynamics of bistable RNAs studied by time-resolved NMR spectroscopy

Philipp Wenter, Luc Reymond

The structural transition between two alternate conformations of bistable RNAs has been characterized by time-resolved NMR spectroscopy. The mechanism, kinetics, and thermodynamics underlying the global structural transition of bistable RNAs were delineate ...

2007

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