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Giovanni Dietler

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Publications associées (149)

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Experimenting with knots

Giovanni Dietler

Knots are interesting objects to experiment since their topological properties are combined with mechanical properties, fluid mechanics or dynamical properties like unknotting under the excitation of the thermal energy. Knots play also a role in biology be ...

SPRINGER HEIDELBERG2019

Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains

Giovanni Dietler, Paolo De Los Rios, Françoise Gisou van der Goot Grunberg, Sandor Kasas, Charles Roduit, Alexandre Yersin, Pascal Steiner

Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existen ...

Elsevier2008

Micro- and nanoscale hierarchical structure of core-shell protein microgels

Giovanni Dietler, Raffaele Mezzenga, Francesco Simone Ruggeri

Protein nanofibrils were first discovered in the context of misfolding and neurodegenerative diseases but have recently been found in naturally occurring functional materials including algal adhesives, bacterial coatings, and even mammalian melanosomes. Th ...

Royal Society of Chemistry2016

Toward an Effective Control of DNA's Submolecular Conformation on a Surface

Giovanni Dietler, Aleksandre Japaridze, Dusan Vobornik, Andrea Cerreta, Ewelina Wioletta Lipiec

DNA adopts different conformations based on its environment. We reveal conditions that either preserve the DNA's physiological B-conformation, even upon its placement in UHV, or lead to a partial B-form to A-form reorganization upon DNA's deposition on a s ...

American Chemical Society2016

Influence of DNA Binding Dyes on Bare DNA Structure Studied with Atomic Force Microscopy

Giovanni Dietler, Aleksandr Benke, Aleksandre Japaridze

Fluorescent dyes are widely used for staining and visualization of DNA in optical microscopy based methods. Even though for some dyes the mechanism of binding is known, how this binding affects DNA remains poorly understood. Here we present a novel experim ...

American Chemical Society2015

Blue surface plasmon propagation along thin gold film-gas interface and its use for sensitive nitrogen dioxide detection

Giovanni Dietler, Serguei Sekatski

We demonstrate effective surface plasmon propagation along thin gold film in a blue spectral range: the measured 0.6 degrees angular width of the plasmon excitation peak corresponds to the propagation length around 7 mu m. For this end, we prepared a speci ...

Elsevier2013

Electrical Excitation of Long-Range Surface Plasmons in PC/OLED Structure with Two Metal Nanolayers

Giovanni Dietler, Serguei Sekatski

A current-driven source of long-range surface plasmons (LRSPs) on a duplex metal nanolayer is reported. Electrical excitation of LRSPs was experimentally observed in a planar structure, where an organic light-emitting film was sandwiched between two metal ...

SHANGHAI JIAO TONG UNIV PRESS2020

An Optical Fiber-Based Nanomotion Sensor for Rapid Antibiotic and Antifungal Susceptibility Tests

Giovanni Dietler, Sandor Kasas, Serguei Sekatski, Jiangtao Zhou, María Inés Villalba, Leonardo Venturelli, Anne-Céline Verenna Kohler

The emergence of antibiotic and antifungal resistant microorganisms represents nowadays a major public health issue that might push humanity into a post-antibiotic/antifungal era. One of the approaches to avoid such a catastrophe is to advance rapid antibi ...

Amer Chemical Soc2024

Detecting nanoscale vibrations as signature of life

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Francesco Simone Ruggeri, Petar Stupar

The existence of life in extreme conditions, in particular in extraterrestrial environments, is certainly one of the most intriguing scientific questions of our time. In this report, we demonstrate the use of an innovative nanoscale motion sensor in life-s ...

National Academy of Sciences2015

Nonkinetic Modeling of the Mechanical Unfolding of Multimodular Proteins: Theory and Experiments

Giovanni Dietler, Serguei Sekatski, Fabrizio Benedetti

We introduce and discuss a novel approach called back-calculation for analyzing force spectroscopy experiments on multimodular proteins. The relationship between the histograms of the unfolding forces for different peaks, corresponding to a different numbe ...

Elsevier2011

Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

Giovanni Dietler, Sandor Kasas, Giovanni Longo, María Inés Villalba, Leonardo Venturelli

Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantile ...

MDPI2021

Scanning near-field optical microscope based on double-resonant fiber probe montage and its operation in liquids - art. no. 67282D

Giovanni Dietler, Serguei Sekatski, Kanat Dukenbayev

The operation of the scanning near-field optical microscope based on the double-resonant montage of a fiber probe onto the tuning fork (working frequency of the latter, that is 32 kHz, coincides with the second resonance frequency of the bending oscillatio ...

SPIE2007

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Giovanni Dietler, Harald Brune, Andrzej Kulik, Nicolas Mermod, Guillaume Witz

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

Future Medicine2014

Real-Time Monitoring of Protein Conformational Changes Using a Nano-Mechanical Sensor

Giovanni Dietler, Paolo De Los Rios, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy, Fabrizio Benedetti, Dusan Vobornik

Proteins can switch between different conformations in response to stimuli, such as pH or temperature variations, or to the binding of ligands. Such plasticity and its kinetics can have a crucial functional role, and their characterization has taken center ...

Public Library of Science2014

A universal fluid cell for the imaging of biological specimens in the atomic force microscope

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy, Charles Roduit

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM ...

Wiley-Blackwell2013

Spatial confinement induces hairpins in nicked circular DNA

Giovanni Dietler, Aleksandre Japaridze

In living cells, DNA is highly confined in space with the help of condensing agents, DNA binding proteins and high levels of supercoiling. Due to challenges associated with experimentally studying DNA under confinement, little is known about the impact of ...

Oxford University Press (OUP)2017

Persistence length and scaling properties of single-stranded DNA adsorbed on modiﬁed graphite

Giovanni Dietler, Guillaume Witz

We have characterized the polymer physics of single-stranded DNA (ssDNA) using atomic force microscopy. The persistence length l(p) of circular ssDNA adsorbed on a modified graphite surface was determined independently of secondary structure. At a very low ...

2009

Single yeast cell nanomotions correlate with cellular activity

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Anton Malovichko

Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical ...

AMER ASSOC ADVANCEMENT SCIENCE2020

Fiber-Tip Polymer Microcantilever for Fast and Highly Sensitive Hydrogen Measurement

Giovanni Dietler, Sandor Kasas, Jiangtao Zhou, Leonardo Venturelli

Hydrogen as an antioxidant gas has been widely used in the medical and biological fields for preventing cancer or treating inflammation. However, controlling the hydrogen concentration is crucial for practical use due to its explosive property when its vol ...

AMER CHEMICAL SOC2020

Nanomechanical bio-sensing for fast and reliable detection of viability and susceptibility of microorganisms

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Leonardo Venturelli, Raphaël Foschia

The metabolic activity of living organisms results in cellular motion on a nanometric scale that can be efficiently detected by micro- and nano- fabricated sensors. Quartz crystal microbalance and atomic force microscopy (AFM) inspired techniques have rece ...

Elsevier2021