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Theo Lasser

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Publications associées (125)

Real-time full field laser Doppler imaging

Marcel Leutenegger, Theo Lasser, Antonio Lopez, Tyler Nerton Thacher, Erica Martin-Williams

We present a full field laser Doppler imaging instrument, which enables real-time in vivo assessment of blood flow in dermal tissue and skin. This instrument monitors the blood perfusion in an area of about 50 cm² with 480 × 480 pixels per frame at a rate ...

2011

Dark field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

The invention relates to an optical coherence microscopy system for fast, phase resolved imaging by means of optical coherence microscopy with decoupled illumination and detection apertures, producing a dark-field effect with an enhanced optical contrast. ...

2011

Apparatus and method for detecting and measuring biomolecular interactions

Theo Lasser, Nicolas Durand

A method and system for the rapid detection of biomolecular interactions, the system comprising a sensing platform which comprises a primary support structure including recesses designed to be located in front of a detection unit, said recesses containing ...

2011

Functional optical coherent imaging platform for medical application e.g. neurosurgery, has active camera unit that captures image of specific region of test subject after transmitting stimulus signals

Iwan Märki, Marcel Leutenegger, Theo Lasser, Martin Villiger, Michael Friedrich, Dimitri Nestor Alice Van De Ville, Erica Martin-Williams

The present invention relates to a functional optical coherent imaging (fOCI) platform comprising at least one active camera unit (ACU) comprising a coherent and/or a partially coherent light source, and means for spectral filtering and imaging a selected ...

2010

Dark-field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...

Optical Society of America2010

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Iwan Märki, Theo Lasser, Kai Johnsson, Claudio Dellagiacoma, Stefan Geissbühler, Sambashiva Banala, Grazvydas Lukinavicius, Noelia Laura Bocchio

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010

Method for displaying field e.g. tumor, of brain of human patient during surgery of brain, involves acquiring topography data representing topography of field, where output image is displayed based on topography data

Marcel Leutenegger, Theo Lasser, Antonio Lopez, Erica Martin-Williams

NOVELTY - The method involves illuminating a field (11) of brain (13) of a patient for acquiring an image sequence of the field. The field is illuminated after stimulating the patient for acquiring another image sequence. The image sequences are evaluated ...

2010

Three-dimensional nano-localization of single fluorescent emitters

Iwan Märki, Theo Lasser, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Image formation and tomogram reconstruction in optical coherence microscopy

Theo Lasser, Martin Villiger

In this work we present a model for image formation in optical coherence microscopy. In the spectral domain detection, each wavenumber has a specific coherent transfer function that samples a different part of the object's spatial frequency spectrum. The r ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Iwan Märki, Theo Lasser, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Towards high resolution optical imaging of beta cells in vivo

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Joan Goulley, Erica Martin-Williams

Endocrine beta cells produce and release insulin in order to tightly regulate glucose homeostasis and prevent metabolic pathologies such as Diabetes Mellitus. Optical imaging has contributed greatly to our current understanding of beta cell structure and f ...

Bentham Science Publishers2010

Object i.e. aneurysm, examining method for brain of patient, involves evaluating sequence of images by associating pixels with analysis image value depending on temporal changes of image values of pixels in specified frequency range

Marcel Leutenegger, Theo Lasser, Antonio Lopez, Michael Friedrich, Erica Martin-Williams

NOVELTY - The method involves illuminating an object i.e. aneurysm, with measuring light with wavelengths ranging from 790 nanometer to 820 nanometer, and recording a sequence of images (21, 22) i.e. white light image, of the illuminated object. The sequen ...

2009

Direct measurement of diffusing proteins in nanochannels using fluorescence correlation spectroscopy

Philippe Renaud, Arnaud Bertsch, Theo Lasser, Nicolas Durand, Raphaël Goetschmann

Transport of proteins in liquid-filled nanometer-sized apertures with charged surfaces based on both diffusion and convective flow is investigated with the use of fluorescence correlation spectroscopy. The measured fluctuations of fluorescence allow the ev ...

Elsevier2009

In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

Theo Lasser, Martin Villiger, Michael Friedrich, Rainer Leitgeb, Anne Grapin-Botton, Joan Goulley

Aims/hypothesis Structural and functional imaging of the islets of Langerhans and the insulin-secreting beta cells represents a significant challenge and a long-lasting objective in diabetes research. In vivo microscopy offers a valuable insight into beta ...

Springer Verlag2009

Steerable filters for orientation estimation and localization of fluorescent dipoles

Iwan Märki, Michaël Unser, Theo Lasser, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy (i.e., PALM, STORM) has enabled optical imaging at nanometer-scale resolutions. The localization algorithms used in these techniques rely on fitting a 2-D Gaussian to the in-focus image of individual fluorophores. For f ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2009

Controlling light at the nanoscale with plasmonic antennas

Christian Santschi, Theo Lasser, Thomas Schmid, Olivier Martin, Holger Fischer, Andreas Kern

2009

High volume confinement in two-photon total-internal- reflection fluorescence correlation spectroscopy

Iwan Märki, Marcel Leutenegger, Theo Lasser, Denis Ivanov

We report results on two-photon total-internal-reflection fluorescence correlation spectroscopy with radially polarized light. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdV ...

AIP American Institute of Physics2009

Imaging of G protein-coupled receptors in solid-supported planar lipid membranes

Marcel Leutenegger, Theo Lasser

This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform for biophysical studies. The authors introduced recently a nove ...

2009

Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters

Iwan Märki, Michaël Unser, Theo Lasser, François Aguet, Stefan Geissbühler

Fluorophores that are fixed during image acquisition produce a diffraction pattern that is characteristic of the orientation of the fluorophore’s underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolu ...

OSA2009

See the Brain at Work—Intraoperative Laser Doppler Functional Brain Imaging

Marcel Leutenegger, Michaël Unser, Theo Lasser, Antonio Lopez, Dimitri Nestor Alice Van De Ville, Rainer Leitgeb, Erica Martin-Williams

During open brain surgery we acquire perfusion images non-invasively using laser Doppler imaging. The regions of brain activity show a distinct signal in response to stimulation providing intraoperative functional brain maps of remarkably strong contrast. ...

2009

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