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Theo Lasser

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Publications associées (125)

Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging

Theo Lasser, Tomas Lukes

Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and pathological condit ...

Nature Research2017

NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes

Theo Lasser, Jérôme Extermann, Miguel Sison

We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT derived peptide enabling cellular uptake as well as mitochondria targeting triphenyl-phosphonium (TPP ...

Royal Soc Chemistry2017

Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Séverine Coquoz, Arno Pino Bouwens, Jérôme Extermann

Optical coherence microscopy (OCM) is an interferometric technique providing 3D images of biological samples with micrometric resolution and penetration depth of several hundreds of micrometers. OCM differs from optical coherence tomography (OCT) in that i ...

2017

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Azat Sharipov, Hendrik Georges Deschout, Tomas Lukes

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Spie-Int Soc Optical Engineering2017

Optical projection tomography for rapid whole mouse brain imaging

Theo Lasser, Antonio Lopez, Dimitri Nestor Alice Van De Ville, Paul James Marchand, Jessica Sordet, David Nguyen, Jérôme Extermann, Miguel Sison, Marcin Antoni Sylwestrzak, Julia Sophie Katarina Nilsson, Arielle Louise Planchette

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of ...

Optical Soc Amer2017

Gastro-intestinal biomarkers for diagnosis and therapies of proteinopathies

Theo Lasser, Tristan Bolmont, Taoufiq Harach

Pharmaceutical composition for the treatment of proteinopathies comprising products which are able to stimulate growth or inhibition of at least one of Odoribacter, Oscillospira, Dehalobacterium, Alistipes, Parabacteroides, Lactobacillus and Sutterella, Fi ...

2016

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic, Lely Feletti, Daniel Pawel Szlag, Azat Sharipov, Hendrik Georges Deschout, Tomas Lukes

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Anne Grapin-Botton, Corinne Berclaz, Paul James Marchand, David Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator

Theo Lasser, Antonio Lopez, Séverine Coquoz, Miguel Sison, Amir Jean Nahas

We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. Afte ...

SPIE Society of Photo-optical Instrumentation Engineers2016

Optical prism for creating multiple views of an image

Marcel Leutenegger, Theo Lasser, Stefan Geissbuehler

Optical prism comprising two external parallel and reflecting surfaces, one internal interface, parallel to said surfaces and acting as light splitter; said prism furthermore comprising an entrance side forming an angle β with respect to said interface and ...

2016

Compressed imaging by sparse random convolution

Theo Lasser, Antonio Lopez, Aurélien Bourquard

The theory of compressed sensing (CS) shows that signals can be acquired at sub-Nyquist rates if they are sufficiently sparse or compressible. Since many images bear this property, several acquisition models have been proposed for optical CS. An interestin ...

Optical Society of America2016

Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

Theo Lasser, Daniel Pawel Szlag

We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM inst ...

SPIE digital Library2016

Fluorescent Nanodiamond–Gold Hybrid Particles for Multimodal Optical and Electron Microscopy Cellular Imaging

Theo Lasser, Miguel Sison, Weina Liu

There is a continuous demand for imaging probes offering excellent performance in various microscopy techniques for comprehensive investigations. of cellular processes by more than, one technique. FluoreScent nanodianiond gold nanoparticles (END Au) consti ...

American Chemical Society (ACS)2016

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)

Theo Lasser, Christophe Pache, Jérôme Extermann, Miguel Sison, Amir Jean Nahas

Spie-Int Soc Optical Engineering2016

Diffraction-unlimited imaging: from pretty pictures to hard numbers

Marcel Leutenegger, Theo Lasser

Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts are now being focused on deriving quantitative ...

Springer Verlag2015

Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers

Theo Lasser, Antonio Lopez, Anne Grapin-Botton, Corinne Berclaz, Christophe Pache, Arno Pino Bouwens, Daniel Pawel Szlag

The identification of a beta-cell tracer is a major quest in diabetes research. However, since MRI, PET and SPECT cannot resolve individual islets, optical techniques are required to assess the specificity of these tracers. We propose to combine Optical Co ...

Nature Publishing Group2015

IMAGING White-light diffraction tomography

Theo Lasser, Arno Pino Bouwens

Nature Publishing Group2014

Optical coherence correlation spectroscopy (OCCS)

Marcel Leutenegger, Theo Lasser, Stéphane Broillet, Stefan Geissbühler, Christophe Pache, Arno Pino Bouwens, Akihiro Sato

We present a new method called optical coherence correlation spectroscopy (OCCS) using nanoparticles as reporters of kinetic processes at the single particle level. OCCS is a spectral interferometry based method, thus giving simultaneous access to several ...

Optical Society of America2014

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Marcel Leutenegger, Theo Lasser, Aurélien Godinat, Françoise Gisou van der Goot Grunberg, Patrick Alain Sandoz, Noelia Laura Bocchio, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

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