Publications de Horst Pick | EPFL Graph Search

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Fluorescence Correlation Spectroscopy (FCS) is a suitable technique to investigate biomol. interactions. The advantage of FCS lies in its wide dynamic range in the time and concn. domain, its sensitivity, and thus in the small amt. of material needed. The characteristic time of the processes that can be measured spans more than eleven orders of magnitude, between several nanoseconds up to hours; the measurable concn. range extends from 0.1 nM to several mM. Here we report on FCS expts. to investigate the pr

Investigating and understanding dynamic biofilm growth mechanisms is challenging, often because state-of-the-art optical characterization tools provide limited information. Micrometric electrochemical imaging of Escherichia coli biofilms using Soft-Probe-S ...

A general approach for the sequential labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor anal. of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates

The development of new biodegradable plastic materials from renewable sources is a major challenge for plastic industries to provide sustainable alternatives to petroleum plastic. Bio-based plastics are generally obtained from photosynthetic biomass such a ...

A target plate for enhanced matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) analysis of intact biological entities. The target plate comprises an electrically conductive substrate (1) covered at least partially with immobilised pho ...

A multi-gene family of ~1000 G protein-coupled olfactory receptors (ORs) constitutes the molecular basis of mammalian olfaction. Due to the lack of structural data its remarkable capacity to detect and discriminate thousands of odorants remains poorly unde ...

The in vivo and in vitro labeling of fusion proteins with synthetic mols. capable of probing and controlling protein function has the potential to become an important method in functional genomics and proteomics. We have recently introduced an approach for the specific labeling of fusion proteins, which is based on the generation of fusion proteins with the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT) and the irreversible reaction of hAGT with O6-benzylguanine derivs. Here, we report

Several lines of evidence point to the presence of both, cellulose and chitin as structural constituents of the Chlorella vulgaris cell wall. These biopolymers are favourable building blocks for many sustainable industrial applications for instance as bioc ...

Transient gene expression (TGE) in mammalian cells at the reactor scale is becoming increasingly important for the rapid production of recombinant proteins. We improved a process for transient calcium phosphate-based transfection of HEK293-EBNA cells in a 1-3 L bioreactor volume. Cells were adapted to suspension culture using a commercially available medium (BioWhittaker, Walkersville, MD). Process parameters were optimized using a plasmid reporter vector encoding the enhanced green fluorescent protein (EGF

Print-Light-Synthesis (PLS) combines the inkjet printing of a ruthenium precursor ink with the simultaneous photo-induced generation of ruthenium oxide films. During PLS, inkjet-printing generates on conductive as well as insulating substrates micrometer-t ...

The invention relates to various embodiments of a bioanalytical reagent with at least one vesicle, generated from a living cell, comprising at least one receptor, characterized in that a mechanism of signal transduction triggered by said receptor in the ce ...

Titanium dioxide-modified target plates were developed to enhance intact bacteria analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The plates were designed to photocatalytically destroy the bacterial envelope struct ...

The recombinant antibodies RB714, RB715, RB716, RB717 and RB718 detect by ELISA the M. smithi histone HMtA protein fused to a GST. ...

Scanning electrochemical microscopy (SECM) has been widely used for the electrochemical imaging of dynamic topographical and metabolic changes in alive adherent mammalian cells. However, extracting intracellular information by SECM is challenging, since it ...

Die Untersuchung von Biomarkern und injizierten theranostischen Nanomaterialien in Geweben und Organen spielt eine zentrale Rolle bei zahlreichen medizinischen Verfahren von der Krebsdiagnose bis hin zur Arzneimittelverabreichung. Zu diesem Zweck kann die ...

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Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy

Soft-probe-scanning electrochemical microscopy reveals electrochemical surface reactivity of E. coli biofilms

Labeling of fusion proteins with synthetic fluorophores in live cells

Algal cellulose, production and potential use in plastics: Challenges and opportunities

Maldi device and methods of preparation and use thereof

Exchanging ligand-binding specificity between a pair of mouse olfactory receptor paralogs reveals odorant recognition principles

Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro

Abiotic stress approaches for enhancing cellulose and chitin production in Chlorella vulgaris

Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

Print-Light-Synthesis of ruthenium oxide thin film electrodes for electrochemical sensing applications

Bioanalytical reagent, method for production thereof, sensor platforms and detection methods based on use of said bioanalytical reagent

Detection of antimicrobial resistance-associated proteins by titanium dioxide-facilitated intact bacteria mass spectrometry

Distribution Plasticity of the Human Estrogen Receptor α in Live Cells: Distinct Imaging of Consecutively Expressed Receptors

RB714, RB715, RB716, RB717 and RB718 antibodies recognize the histone HMtA protein from Methanobrevibacter smithii by ELISA

Fixation and Permeabilization Approaches for Scanning Electrochemical Microscopy of Living Cells

Weiche elektrochemische Sonden zum Abbilden der Verteilung von Biomarkern und injizierten Nanomaterialien in tierischem und menschlichem Gewebe

Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs

Towards an affinity-based capture system for the isolation of high expression cells using a co-expressed surface protein