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Florian Maria Wurm

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Publications associées (117)

CHO Quasispecies—Implications for Manufacturing Processes

Florian Maria Wurm

Chinese hamster ovary (CHO) cells are a source of multi-ton quantities of protein pharmaceuticals. They are, however, immortalized cells, characterized by a high degree of genetic and phenotypic diversity. As is known for any biological system, this divers ...

MDPI2013

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Stéphanie Tissot, Clara Joséphine Douet

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefﬁcient of oxygen (kLa) was 200%–400% higher in the HT tu ...

2013

Numerical simulation of orbitally shaken viscous fluids with free surface

Florian Maria Wurm, Alfio Quarteroni, Marco Discacciati, David Hacker, Stéphanie Tissot, Samuel Quinodoz

Orbitally shaken bioreactors are an emerging alternative to stirred-tank bioreactors for large-scale mammalian cell culture, but their fluid dynamics is still not well defined. Among the theoretical and practical issues that remain to be resolved, the char ...

Wiley-Blackwell2013

Study of the improved Sf9 transient gene expression process

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TG ...

2013

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sarah Thurnheer

Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfect ...

Elsevier2013

The optimization of a rapid low-cost alternative of large-scale medium sterilization

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Dominique Terry Monteil Jr

2013

DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Harm-Anton Klok, Zuzana Kadlecova, Yashas Rajendra

PEI and polylysine are among the most investigated synthetic polymeric carriers for DNA delivery. Apart from their practical use, these 2 classes of polymers are also of interest from a fundamental point of view as they both can be prepared in different ar ...

Elsevier2013

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, Simone Schmitt, Yury Tsybin, Luca Fornelli, Julien Parra

In this study, a recombinant monoclonal IgG antibody was produced by transient gene expression (TGE) in suspension-adapted HEK-293E cells. The objective of the study was to determine the variation in recombinant IgG yield and glycosylation in ten independe ...

Elsevier2012

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Harm-Anton Klok, Zuzana Kadlecova, Yashas Rajendra

The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in I ...

2012

Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Harm-Anton Klok, Zuzana Kadlecova

Lysine-based polycations are widely used as nonviral carriers for gene delivery. This manuscript reports the results of a comparative study on the in vitro cytotoxicity of a library of three structural polylysine variants, namely, linear polylysine (LPL), ...

American Chemical Society2012

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Mélanie Hubert

We describe a rapid method for monitoring the cell growth and decline phases in suspension cultures of animal cells. During the cell growth phase, ultraviolet (UV)-absorbing components in the medium are consumed, but at later times as cells begin to die, U ...

Elsevier2012

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sagar Shashidhar Manoli

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Stéphanie Tissot

The aim of this study was to gain a better understanding of orbitally shaken bioreactors (OSRs) operated without controllers for pH and dissolved oxygen (DO) concentration. We used cylindrical OSRs with working volumes ranging from 250mL to 200L to determi ...

Elsevier2012

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker, Harm-Anton Klok, Zuzana Kadlecova

Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI Max) regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI ...

Wiley-Blackwell2012

Large-Scale Transfection of Mammalian Cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Carine Meerschman

The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneim ...

Humana Press2011

Hydrodynamic stress in orbitally shaken bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Alfio Quarteroni, Mohamed Farhat, Marco Discacciati, David Hacker, Stéphanie Tissot, Martino Reclari, Samuel Quinodoz, Matthieu Dreyer, Dominique Terry Monteil Jr

2011

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

2011

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sagar Shashidhar Manoli

2011

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Yashas Rajendra, Joao Nuno Dos Santos Pereira

2011

Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry

Florian Maria Wurm, Sophie Nallet, Yury Tsybin, Luca Fornelli, Julien Parra

Improving qualitative and quantitative characterization of monoclonal antibodies is essential, because of their increasing popularity as therapeutic drug targets. Electron transfer dissociation (ETD)-based top-down mass spectrometry (MS) is the method of c ...

2011

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