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Florian Maria Wurm

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Publications associées (117)

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

Florian Maria Wurm, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Likuan Zhu

Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50-mL tubes with a ventilated cap (OSR50), origina ...

American Chemical Society2017

Validation of Scalable and Compliant AAV Production

Florian Maria Wurm, Nicole Déglon, Bernard Schneider, Gabriel Vachey, Daniel Roman Blessing

Cell Press2017

User-Friendly Image-Based Segmentation and Analysis of Chromosomes

Florian Maria Wurm, Michaël Unser, Lucia Baldi Unser, Virginie Sophie Uhlmann, Ricard Delgado Gonzalo

We designed two efficient and user-friendly tools for the segmentation and analysis of images containing chromosomes or, more generally, rod-shaped elements that are spread on microscopic slides. The segmentation tool allows to automatically extract the pr ...

IEEE2016

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Giulia Tontodonati

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Super ...

Elsevier2016

Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Yashas Rajendra, Sowmya Balasubramanian

Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposonspiggyBac, Tol2, and Sleeping Beautyfor their ability to generate c ...

Wiley-Blackwell2016

Multigene expression in stable CHO cell pools generated with the piggyBac transposon system

Florian Maria Wurm, David Hacker, Sowmya Balasubramanian

Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...

American Chemical Society2016

Manufacture of Recombinant Therapeutic Proteins Using Chinese Hamster Ovary Cells in Large-Scale Bioreactors History, Methods, and Perspectives

Florian Maria Wurm

In the last three decades, protein yields from recombinant CHO cells in bioreactors have increased 10-100 fold, as the result of improvements in media, bioprocess design and cell culture process control. The contribution of expression vector constructs and ...

Wiley2016

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Marc Moniatte, Valentin Juvet, Dominique Terry Monteil Jr

Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...

American Chemical Society2016

Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors

Florian Maria Wurm, Nicole Déglon, Bernard Schneider, Daniel Roman Blessing

Nature Publishing Group2016

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Zuzana Kadlecova, Sowmya Balasubramanian

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...

Elsevier2015

A simple plasmid-based transient gene expression method using High Five cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann, Xiao Shen

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Elsevier2015

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sowmya Balasubramanian

Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to reduce the pDNA amou ...

American Chemical Society2015

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

Transient gene expression (TGE) in human embryonic kidney (HEK-293) and Chinese hamster ovary (CHO) cells is a well-established technology for the rapid generation of recombinant proteins. Although the maximum TGE yields have reached 1 g/L or more, the amo ...

American Chemical Society2015

Production of active glycosylation-deficient gamma-secretase complex for crystallization studies

Florian Maria Wurm, David Hacker, Patrick Fraering, Mitko Dimitrov, Andres Ricardo Lopez Robles, Hermeto Gerber, Virginie Sandra Braman

Alzheimer's disease (AD)-associated gamma-secretase is a ubiquitously expressed multi-subunit protease complex embedded in the lipid bilayer of cellular compartments including endosomes and the plasma membrane. Although gamma-secretase is of crucial intere ...

Wiley-Blackwell2015

Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Nicole Déglon, Bernard Schneider, Monique Montavon, Daniel Roman Blessing

Nature Publishing Group2015

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Florian Maria Wurm, Lucia Baldi Unser, Yashas Rajendra, Fatemeh Davami

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of ...

2014

Virus-free transient protein production in Sf9 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

A method for virus-free transient gene expression from suspension-adapted Sf9 insect cells was developed with the gene of interest being expressed from a plasmid carrying the homologous region 5 enhancer (hr5) and the immediate early 1 (ie1) promoter from ...

Elsevier2014

Highly efficient production of the Alzheimer's γ-Secretase integral membrane protease complex by a multi-gene stable integration approach

Florian Maria Wurm, David Hacker, Patrick Fraering, Lorène Aeschbach, Mitko Dimitrov, Sowmya Balasubramanian

Inefficient production of membrane-embedded multi-protein complexes by conventional methods has largely prevented the generation of high-resolution structural information and the performance of high-throughput drug discovery screens for this class of prote ...

2013

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into ...

Elsevier2013

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Saroj Ghimire, Dominique Terry Monteil Jr, Giulia Tontodonati

Orbitally shaken bioreactors (OSRs) support the efficient cultivation of mammalian cells in suspension at various scales of operation. Here we introduce a disposable culture vessel with a ventilated cap having a nominal volume of 600 mL (TubeSpin (R) biore ...

Elsevier2013

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