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Florian Maria Wurm

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Related publications (116)

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Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1,000 liters

Florian Maria Wurm, Alfio Quarteroni, Mathieu Benjamin Stettler, Marco Discacciati, Nicola Parolini, David Hacker, Xiuwei Zhang

Cylindrical containers agitated by orbital shaking are being developed as simple and cost-effective bioreactor systems for the cultivation of mammalian cells. Here the oxygen transfer capacities of containers with nominal volumes from 50 mL to 2000 L were ...

2009

Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing

Florian Maria Wurm

Recombinant proteins have been produced for over 30 years. Applications range from enzymes used in laundry detergents to antigen-detecting antibodies in cancer therapy. Despite similarities in manufacturing, drastic differences in retail pricing between re ...

2019

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

Sensitivity of the SHiP experiment to Heavy Neutral Leptons

Florian Maria Wurm, Aurelio Bay, Mikhail Chapochnikov, Guido Haefeli, Alexey Boyarsky, Sun Hee Kim, Oleg Ruchayskiy, Nathalie Sophie Adeline Serra, Lesya Shchutska, Adriano Pastore, Nikolaos Charitonidis, Johannes Richard Cornelis Geerit Kruis, Erica Manfredi, Sargis Hakobyan, Eirini Koukovini Platia, Jean-Loup Tastet, Ekaterina Kuznetsova, Inar Timiryasov, Maria Elena Stramaglia, Federico Leo Redi, Elena Graverini

Heavy Neutral Leptons (HNLs) are hypothetical particles predicted by many extensions of the Standard Model. These particles can, among other things, explain the origin of neutrino masses, generate the observed matter-antimatter asymmetry in the Universe an ...

Springer Nature2019

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Florian Maria Wurm, Lucia Baldi Unser, Yashas Rajendra

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of ...

2014

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Saroj Ghimire, Dominique Terry Monteil Jr

Orbitally shaken bioreactors (OSRs) support the efficient cultivation of mammalian cells in suspension at various scales of operation. Here we introduce a disposable culture vessel with a ventilated cap having a nominal volume of 600 mL (TubeSpin (R) biore ...

Elsevier2013

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Florian Maria Wurm, Madiha Derouazi, Martin Bertschinger, David Hacker

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification sys ...

2006

Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors

Christos Comninellis, Florian Maria Wurm, Mohamed Farhat, David Hacker

Efficient mixing in bioreactors is essential in order to avoid concentration gradients which can be harmful for mammalian cells. To study mixing and its scalability in orbitally shaken cylindrical bioreactors, we measured mixing times in containers with no ...

Elsevier2010

Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Recombinant therapeutic proteins produced in mammalian cells represent a major class of biopharmaceuticals. In recent years, their demand has increased dramatically and is now driving the development of a variety of improvements to maximize their expressio ...

Elsevier2008

Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names

Florian Maria Wurm

Chinese Hamster Ovary [CHO] cells are the workhorse for production of modern biopharmaceuticals. They are however immortalized cells with a high propensity for genetic change. Judging from published culture records, CHO cell populations have undergone hund ...

2021

A simple high-yielding process for transient gene expression in CHO cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

Here we describe a simplified method for transient gene expression (TGE) in suspension-adapted Chinese hamster ovary (CHO) cells using polyethylenimine (PEI) for DNA delivery. Both the transfection and production phases of the bioprocess were performed at ...

Elsevier2011

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Natalie Muller, David Hacker, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

Analysis of volumetric mass transfer coefficient (k(L)a) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors

Florian Maria Wurm, David Hacker

In this study, the combination of dimensional analysis (DA) and analysis of variance (ANOVA) was used to predict the volumetric mass transfer coefficient (k(L)a) values under different operating conditions for orbitally shaken bioreactors (OSRs) with diffe ...

SPRINGER HEIDELBERG2020

A collagen-poly(lactic acid-co-epsilon-caprolactone) hybrid scaffold for bladder tissue regeneration

Florian Maria Wurm, Jeffrey Alan Hubbell, Peter Frey

Scaffold materials should favor cell attachment and proliferation, and provide designable 3D structures with appropriate mechanical strength. Collagen matrices have proven to be beneficial scaffolds for tissue regeneration. However, apart from small intest ...

2011

Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

Florian Maria Wurm, David Hacker

Lentivirus-derived vectors (LVs) were studied for the generation of stable recombinant Chinese hamster ovary (CHO) cell lines. Stable pools and clones expressing the enhanced green fluorescent protein (eGFP) were selected via fluorescence-activated cell so ...

Wiley-Blackwell2010

Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold rel ...

Springer Netherlands2010

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Florian Maria Wurm, Mathieu Benjamin Stettler, David Hacker

Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese h ...

American Chemical Society2007

A simple plasmid-based transient gene expression method using High Five cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann, Xiao Shen

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Elsevier2015

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Florian Maria Wurm, Madiha Derouazi, David Hacker, Sébastien Chenuet

Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (G ...

2008

Numerical simulation of orbitally shaken viscous fluids with free surface

Florian Maria Wurm, Alfio Quarteroni, Marco Discacciati, David Hacker, Samuel Quinodoz

Orbitally shaken bioreactors are an emerging alternative to stirred-tank bioreactors for large-scale mammalian cell culture, but their fluid dynamics is still not well defined. Among the theoretical and practical issues that remain to be resolved, the char ...

Wiley-Blackwell2013