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Pierre Marquet

Cette personne a quitté l’EPFL

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Publications associées (64)

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Marker-free phase nanoscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz, Bokyoung Kim

This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging ...

AMER CHEMICAL SOC2023

Recognition and classification of red blood cells using digital holographic microscopy and data clustering with discriminant analysis

Pierre Marquet, Daniel Boss

We propose to apply statistical clustering algorithms on a three-dimensional profile of red blood cells (RBCs) obtained through digital holographic microscopy (DHM). We show that two classes of RBCs stored for 14 and 38 days can be effectively classified. ...

2011

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Pascal Jourdain

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Nicolas Aspert, Frédéric Montfort, Stéphane Bourquin, Mihaela Marian

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, m ...

2006

Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor

Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Daniel Boss, Pascal Jourdain

Chloride channels represent a group of targets for major clinical indications. However, molecular screening for chloride channel modulators has proven to be difficult and time-consuming as approaches essentially rely on the use of fluorescent dyes or invas ...

Public Library of Science2012

Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Nicolas Aspert, Frédéric Montfort

We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such ...

2006

Automated segmentation of multiple red blood cells with digital holographic microscopy

Pierre Marquet, Daniel Boss

We present a method to automatically segment red blood cells (RBCs) visualized by digital holographic microscopy (DHM), which is based on the marker-controlled watershed algorithm. Quantitative phase images of RBCs can be obtained by using off-axis DHM alo ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Comparisons of verbal fluency brain correlates between adults and adolescents suffering from schizophrenia spectrum disorders: A pilot study

Pierre Magistretti, Pierre Marquet

Background and Objectives: Prefrontal cortex (PFC) dysfunctions leading to cognitive deficits refer to a core feature of schizophrenia spectrum disorders (SSD). This exploratory study compares the effect of SSD on two stages of maturation of PFC. Methods: ...

European Journal Of Psychiatry2016

Characterization of microlenses by digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing. Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers. Measurements performed ...

2006

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Daniel Boss

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, posses ...

Elsevier2009

L-Lactate protects neurons against excitotoxicity: implication of an ATP-mediated signaling cascade

Pierre Magistretti, Pierre Marquet, Igor Allaman, Pascal Jourdain

Converging experimental data indicate a neuroprotective action of L-Lactate. Using Digital Holographic Microscopy, we observe that transient application of glutamate (100 μM; 2 min) elicits a NMDA-dependent death in 65% of mouse cortical neurons in culture ...

Nature Research2016

High throughput second harmonic imaging for label-free biological applications

Pierre Magistretti, Pierre Marquet, Aleksandra Radenovic, Sylvie Roke, Pascal Jourdain, Orly Bagunu Tarun, Carlos Alberto Macias Romero, Vitalijs Zubkovs, Marie Eve Pascale Didier

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Pierre Marquet, Christian Depeursinge, Chen Liu

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object ...

2019

Increased activation in Broca's area after cognitive remediation in schizophrenia

Pierre Magistretti, Pierre Marquet

Functional magnetic resonance imaging (fMRI) was used to measure changes in cerebral activity in patients with schizophrenia after participation in the Cognitive Remediation Program for Schizophrenia and other related disorders (RECOS). As RECOS therapists ...

Elsevier2014

Marker-Free Automatic Quantification of Drug-Treated Cardiomyocytes with Digital Holographic Imaging

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz, Fabien Kuttler, Bokyoung Kim

We use quantitative phase digital holographic microscopy (QP-DHM) to image and quantify the beating movement of cardiomyocytes, derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. The development of an analysis algo ...

AMER CHEMICAL SOC2020

Shot noise influence in reconstructed phase images SNR in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Etienne Cuche, Florian Charrière

We present a study based on simulations describing how shot noise, an intrinsic part of the recording process with a digital camera, influences the quality of the reconstructed phase images under different beams intensities configurations. ...

SPIE2005

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Benjamin Rappaz, Florian Charrière

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007