Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9 +/- 8.9 nm and 4.7 +/- 0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively. (C) 2009 Elsevier Inc. All rights reserved.