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Pierre Marquet

Cette personne a quitté l’EPFL

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Publications associées (64)

Page 1 sur 4

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Florian Charrière, Frédéric Montfort, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Image-Based Marker-Free Screening of GABA(A) Agonists, Antagonists, and Modulators

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz, Pascal Jourdain, Fabien Kuttler, Damiano Banfi

The ionotropic GABA(A) receptors represent the main target for different groups of widely used drugs having hypnotic and anxiolytic effects. So far, most approaches used to assess GABA activity involve invasive low -throughput electrophysiological techniqu ...

2019

Purely numerical compensation for microscope objective phase curvature in digital holographic microscopy: influence of digital phase mask position

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

Introducing a microscope objective in an interferometric setup induces a phase curvature on the resulting wavefront. In digital holography, the compensation of this curvature is often done by introducing an identical curvature in the reference arm and the ...

2006

Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells

Pierre Marquet, Daniel Boss

In this paper, we present an automated approach to quantify information about three-dimensional (3D) morphology, hemoglobin content and density of mature red blood cells (RBCs) using off-axis digital holographic microscopy (DHM) and statistical algorithms. ...

Optical Society of America2012

Shot noise influence in reconstructed phase image SNR in digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

In digital holographic microscopy, shot noise is an intrinsic part of the recording process with the digital camera. We present a study based on simulations and real measurements describing the shot-noise influence in the quality of the reconstructed phase ...

2006

High b-value diffusion-weighted imaging: a sensitive method to reveal white matter differences in schizophrenia

Jean-Philippe Thiran, Patric Hagmann, Reto Meuli, Leila Cammoun, Djalel Eddine Meskaldji, Pierre Marquet, Leila Faiza Ben Hamida Cammoun, Philipp Baumann

Over the last ten years, Diffusion-weighted Imaging (DWI) has become an important tool to investigate white matter (WM) anomalies in schizophrenia. Despite technological improvement and the exponential use of this technique, discrepancies remain and little ...

Elsevier2012

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Nicolas Pavillon, Benjamin Rappaz, Pascal Jourdain, Daniel Boss

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

Society for Neuroscience2011

Spatially-Resolved Eigenmode Decomposition of Red Blood Cells Membrane Fluctuations Questions the Role of ATP in Flickering

Dimitri Nestor Alice Van De Ville, Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Daniel Boss

Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of ...

Public Library Science2012

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz, Michel Prudent, Manon Sandra Bardyn

We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell me ...

2018

Dual action of L-Lactate on the activity of NR2B-containing NMDA receptors: from potentiation to neuroprotection

Pierre Magistretti, Pierre Marquet, Igor Allaman, Pascal Jourdain

L-Lactate is a positive modulator of NMDAR-mediated signaling resulting in plasticity gene induction and memory consolidation. However, L-Lactate is also able to protect neurons against excito-toxic NMDAR activity, an indication of a mitigating action of L ...

Nature Research2018

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Pascal Jourdain, Daniel Boss

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Automated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy

Pierre Marquet, Gerardo Turcatti, Benjamin Rappaz

Compounds tested during drug development may have adverse effects on the heart; therefore all new chemical entities have to undergo extensive preclinical assessment for cardiac liability. Conventional intensity-based imaging techniques are not robust enoug ...

Optical Society of America2015

Polarization microscopy by use of digital holography: application to optical- fiber birefringence measurements

René Salathé, Hans Georg Limberger, Pierre Marquet, Christian Depeursinge, Tristan Colomb, Etienne Cuche, Florian Dürr

We present a digital holographic microscope that permits one to image polarization state. This technique results from the coupling of digital holographic microscopy and polarization digital holography. The interference between two orthogonally polarized re ...

2005

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Pierre Marquet, Christian Depeursinge, Jonas Kühn, Tristan Colomb, Etienne Cuche, Florian Charrière, Frédéric Montfort

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off- axis geometry, of the interfe ...

2006

Automated quantitative analysis of 3D morphology and mean corpuscular hemoglobin in human red blood cells stored in different periods

Pierre Marquet, Daniel Boss

Quantitative phase (QP) images of red blood cells (RBCs), which are obtained by off-axis digital holographic microscopy, can provide quantitative information about three-dimensional (3D) morphology of human RBCs and the characteristic properties such as me ...

Optical Soc Amer2013

Signification of the phase function parameter gamma explored with a fractal distribution of scatterers

Pierre Marquet, Christian Depeursinge, Stéphane Chamot, Elena Migacheva

The optical properties within limited volumes of diffusive media can be probed by carrying spatially-resolved measurements of diffused light at short source-detector separation (typically one scattering mean free path). At such distance, analytical models ...

Optical Society of America2010

The human CFTR protein expressed in CHO cells activates aquaporin-3 in a cAMP-dependent pathway: study by digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Sylvain Eric Lengacher, Pascal Jourdain

The transmembrane water movements during cellular processes and their relationship to ionic channel activity remain largely unknown. As an example, in epithelial cells it was proposed that the movement of water could be directly linked to cystic fibrosis t ...

Company of Biologists2014

Simultaneous cell morphometry and refractive index measurement with dual- wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Florian Charrière

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelengt ...

2008

Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Benjamin Rappaz, Etienne Cuche

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. ...

2005