Recherche

Afficher tous les résultats pour

Accueil

Personne

Matthias Lütolf

Cette personne a quitté l’EPFL

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (176)

Page 7 sur 9

Programmable microfluidic patterning of protein gradients on hydrogels

Matthias Lütolf, Steffen Cosson, Simone Allazetta

Computer-controlled hydrodynamic flow focusing was utilized to generate tethered protein gradients of any user-defined shape on the surface of soft synthetic hydrogels. ...

Royal Society of Chemistry2010

Microfluidics-generated immobilized biomolecule gradients on hydrogels

Matthias Lütolf, Steffen Cosson, Stefan Kobel

Extra-cellular matrix (ECM) tethered morphogen gradients play an important role in tissue development and regeneration. There is currently only a limited set of model systems which can mimic these gradients in an artificial ECM-like environment. We have de ...

The Chemical and Biological Microsystems Society2008

Establishment of a microwell-array-based miniaturized thymic organoid model suitable for high-throughput applications

Matthias Lütolf, Tania Hübscher

T cell development depends critically on the thymic stroma—in particular, the diverse array of functionally distinct thymic epithelial cell (TEC) types. However, a robust in vitro thymus model mimicking the native thymus and compatible with medium-/high-th ...

2025

Substrate elasticity modulates the responsiveness of mesenchymal stem cells to commitment cues

Matthias Lütolf, Samy Gobaa

Fate choices of stem cells are regulated in response to a complex array of biochemical and physical signals from their microenvironmental niche. Whereas the molecular composition and the role of mechanical niche cues have been extensively studied, relative ...

Royal Soc Chemistry2015

Tissue-Engineering the Intestine: The Trials before the Trials

Matthias Lütolf

Building complex tissues requires the development of innovative interdisciplinary engineering solutions. In this Forum, the INTENS Consortium discuss experimental considerations and challenges for generating a tissue-engineered intestine for the treatment ...

CELL PRESS2019

The hope and the hype of organoid research

Matthias Lütolf

The recent increase in organoid research has been met with great enthusiasm, as well as expectation, from the scientific community and the public alike. There is no doubt that this technology opens up a world of possibilities for scientific discovery in de ...

Company of Biologists2017

Specification of haematopoietic stem cell fate via modulation of mitochondrial activity

Johan Auwerx, Matthias Lütolf, Olaia Maria Naveiras Torres-Quiroga, Mukul Girotra, Vasco Ledebur de Antas de Campos, Aline Roch, Nicola Vannini, Sonja Giger, Gennady Nikitin

Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. However, whether this change in the metabolic program is the cause ...

Nature Publishing Group2016

High-Fidelity Printing Strategies for Printing 3D Vascular Hydrogel Structures

Jürgen Brugger, Philippe Renaud, Matthias Lütolf, Thomas Michael Braschler, Kristopher Pataky

Vascularization arguably poses the most significant hurdle for the success of most biomaterials-based tissue engineering therapies. In this work, we report two printing strategies that permit the 3D Inkjet printing of fluorescent alginate hydro gels into o ...

Soc Imaging Science & Technology, 7003 Kilworth Lane, Springfield, Va 22151 Usa2009

A Single Metabolite which Modulates Lipid Metabolism Alters Hematopoietic Stem/Progenitor Cell Behavior and Promotes Lymphoid Reconstitution

Matthias Lütolf, Sonja Giger, Marlen Knobloch

Fatty acid beta-oxidation (FAO), the breakdown of lipids, is a metabolic pathway used by various stem cells. FAO levels are generally high during quiescence and downregulated with proliferation. The endogenous metabolite malonyl-CoA modulates lipid metabol ...

2020

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice

Matthias Lütolf, Saba Rezakhani, Antonius Chrisnandy

Glandular epithelia, including mammary gland (MG) and prostate, are composed of luminal and basal cells. During embryonic development, glandular epithelia arise from multipotent stem cells (SCs) that are replaced after birth by unipotent basal and unipoten ...

Nature Research2024

Understanding the Mechanobiology of Early Mammalian Development through Bioengineered Models

Matthias Lütolf, Stefano Davide Vianello

Research in developmental biology has been recently enriched by a multitude of in vitro models recapitulating key milestones of mammalian embryogenesis. These models obviate the challenge posed by the inaccessibility of implanted embryos, multiply experime ...

Cell Press - Elsevier2019

Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment

Matthias Lütolf, Olaia Maria Naveiras Torres-Quiroga, Aline Roch, Philipp Sebastian Lienemann, Stéphanie Elisabeth Metzger, Queralt Vallmajó Martín

The treatment of bone defects with recombinant bone morphogenetic protein-2 (BMP-2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low-dose BMP-2-based bone regeneration by mob ...

WILEY2020

High-throughput stem cell-based phenotypic screening through microniches

Matthias Lütolf, Simone Allazetta, Mehmet Ugur Girgin, Laura Kolb

As the field of tissue engineering develops, methods for screening combinations of signals for their effects on stem cell behavior are needed. We introduce a microgel-based screening platform for testing combinations of in situ-generated proteins on stem c ...

ROYAL SOC CHEMISTRY2019

Biomaterials: Spotlight on hydrogels

Matthias Lütolf

By using light to control the degradation of hydrogel components in space and time, researchers have generated a tool to help them reconstruct functional biological tissues in a culture dish. ...

2009

Bioengineered Tubular Biliary Organoids

Matthias Lütolf, Saba Rezakhani, Mikhail Nikolaev, Bilge Sen Elçi

Liver organoids have emerged as promising in vitro models for toxicology, drug discovery, and disease modeling. However, conventional 3D epithelial organoid culture systems suffer from significant drawbacks, including limited culture duration, a nonphysiol ...

Wiley2024

Assessing the precision of morphogen gradients in neural tube development

Matthias Lütolf, Nathalie Brandenberg

Nature Portfolio2024

Defined three-dimensional microenvironments boost induction of pluripotency

Massimiliano Caiazzo, Matthias Lütolf, Alessandra Piersigilli, Adrian Ranga, Yoji Tabata, Yuya Okawa

Since the discovery of induced pluripotent stem cells (iPSCs), numerous approaches have been explored to improve the original protocol, which is based on a two-dimensional (2D) cell-culture system. Surprisingly, nothing is known about the effect of a more ...

Nature Publishing Group2016

Method and device for mixing two streams of droplets

Matthias Lütolf, Yoji Tabata, Andrea Negro

The invention relates to a method for mixing a first stream of droplets (1) with at least one second stream of droplets (2) to obtain a third stream of fused droplets (3), by collision and fusion of at least one droplet of the first stream (1) with at leas ...

2016

Cell-Instructive Microgels with Tailor-Made Physicochemical Properties

Matthias Lütolf, Simone Allazetta, Laura Kolb

A microfluidic in vitro cell encapsulation platform to systematically test the effects of microenvironmental parameters on cell fate in 3D is developed. Multiple cell types including fibroblasts, embryonic stem cells, and cancer cells are incorporated in e ...

Wiley-V C H Verlag Gmbh2015

3D chemical characterization of frozen hydrated hydrogels using ToF-SIMS with argon cluster sputter depth profiling

Matthias Lütolf

Hydrogels have been used extensively in bioengineering as artificial cell culture supports. Investigation of the interrelationship between cellular response to the hydrogel and its chemistry ideally requires methods that allow characterization without labe ...

Amer Inst Physics2016