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Matthias Lütolf

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Publications associées (180)

Development of bioactive hydrogel capsules for the 3D expansion of pluripotent stem cells in bioreactors

Matthias Lütolf, Yoji Tabata

Pluripotent stem cells hold great promise for many pharmaceutical and therapeutic applications. However, the lack of scalable methodologies to expand these cells to clinically relevant numbers is a major roadblock in realizing their full potential. To addr ...

Royal Soc Chemistry2014

3D growth factor patterned cell substrates to locally control cellular behavior

Matthias Lütolf, Vincent Milleret, Yannick Robert Devaud, Philipp Sebastian Lienemann, Martin Ehrbar

Wiley-Blackwell2014

Microfluidic Patterning of Protein Gradients on Biomimetic Hydrogel Substrates

Matthias Lütolf, Steffen Cosson

This protocol describes a versatile microfluidic method to generate tethered protein gradients of virtually any user-defined shape on biomimetic hydrogel substrates. It can be applied to test, in a microenvironment of physiologically relevant stiffness, ho ...

Elsevier Academic Press Inc2014

Biomaterials Approaches in Stem Cell Mechanobiology

Matthias Lütolf, Nikolche Gjorevski

Multiple molecular and physical cues engage in complex interplay to afford the fine control over stem cell behavior that is required during tissue development, homeostasis, and repair. As a result of a close collaboration between biologists, engineers, and ...

Elsevier Academic Press Inc2014

Bioengineering approaches to guide stem cell-based organogenesis

Matthias Lütolf, Adrian Ranga, Nikolche Gjorevski

During organogenesis, various molecular and physical signals are orchestrated in space and time to sculpt multiple cell types into functional tissues and organs. The complex and dynamic nature of the process has hindered studies aimed at delineating morpho ...

Company of Biologists2014

Microscale patterning of hydrogel stiffness through light-triggered uncaging of thiols

Matthias Lütolf, Katarzyna Mosiewicz, Laura Kolb

Mammalian cell behavior is strongly influenced by physical and chemical cues originating from the extracellular matrix (ECM). In vivo, ECM signals are displayed in a spatiotemporally complex fashion, often composed as gradients and in concentration profile ...

Royal Soc Chemistry2014

Instructing stem cell fate by engineered niches

Matthias Lütolf

Wiley-Blackwell2014

Drug discovery through stem cell-based organoid models

Matthias Lütolf, Adrian Ranga, Nikolche Gjorevski

The development of new drugs is currently a long and costly process in large part due to the failure of promising drug candidates identified in initial in vitro screens to perform as intended in vivo. New approaches to drug screening are being developed wh ...

Elsevier2014

Artificial three-dimensional niches deconstruct pancreas development in vitro

Matthias Lütolf, Anne Grapin-Botton, Chiara Greggio, Adrian Ranga, Samy Gobaa, Filippo De Franceschi

In the context of a cellular therapy for diabetes, methods for pancreatic progenitor expansion and subsequent differentiation into insulin-producing beta cells would be extremely valuable. Here we establish three-dimensional culture conditions in Matrigel ...

Company Of Biologists Ltd2013

Microfluidic Synthesis of Cell-Type-Specific Artificial Extracellular Matrix Hydrogels

Matthias Lütolf, Tanja Cloé Hausherr, Simone Allazetta

Droplet microfluidic technology is applied for the high-throughput synthesis via Michael-type addition of reactive, micrometer-sized poly(ethylene glycol) (PEG) hydrogels (“microgels”) with precisely controlled dimension and physicochemical properties. A v ...

American Chemical Society2013

Heparin-binding domain of fibrin(ogen) binds growth factors and promotes tissue repair when incorporated within a synthetic matrix

Matthias Lütolf, Mikaël Martino, Jeffrey Alan Hubbell, Priscilla Suhasna Maithili Briquez, Adrian Ranga

By binding growth factors (GFs), the ECM tightly regulates their activity. We recently reported that the heparin-binding domain II of fibronectin acts as a promiscuous high-affinity GF-binding domain. Here we hypothesized that fibrin, the provisional ECM d ...

National Academy of Sciences2013

In situ cell manipulation through enzymatic hydrogel photopatterning

Matthias Lütolf, Mikaël Martino, Jeffrey Alan Hubbell, Katarzyna Mosiewicz, Martin Ehrbar, Laura Kolb

The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus ...

Nature Publishing Group2013

High-Throughput Screening Of Stem Cell Microenvironments

Matthias Lütolf

Wiley-Blackwell2013

Metabolic control of adult neural stem cell activity by Fasn-dependent lipogenesis

Matthias Lütolf, Marlen Knobloch

Mechanisms controlling the proliferative activity of neural stem and progenitor cells (NSPCs) have a pivotal role to ensure life-long neurogenesis in the mammalian brain(1). How metabolic programs are coupled with NSPC activity remains unknown. Here we sho ...

Nature Publishing Group2013

Cell specific ingrowth hydrogels

Matthias Lütolf

Extracellular mimetic hydrogels formed from peptide crosslinkers and polyethylene glycol monomers permit cell-controlled invasion. The use of matrix metalloproteinase specific peptides might further allow for selective control of different cell-type invasi ...

Elsevier Sci Ltd2013

Patterning of cell-instructive hydrogels by hydrodynamic flow focusing

Matthias Lütolf, Steffen Cosson, Simone Allazetta

Microfluidic gradient systems offer a very precise means to probe the response of cells to graded biomolecular signals in vitro, for example to model how morphogen proteins affect cell fate during developmental processes. However, existing gradient makers ...

Royal Society of Chemistry2013

Live mammalian cell arrays

Matthias Lütolf, Sebastian Maerkl, Samy Gobaa, Luis Miguel Fidalgo Rabanera, Kristina Pan Woodruff

High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We ...

Nature Publishing Group2013

Method for preparing topographically structured microarrays

Matthias Lütolf, Samy Gobaa

A method for preparing a topographically structured hydrogel microarray is described comprising the steps of a) providing one or more types of biomolecule(s) on top of micropillars of an array of micropillars, preferably by means of robotical spotting, b) ...

2013

A Versatile Approach to Engineering Biomolecule-Presenting Cellular Microenvironments

Matthias Lütolf, Philipp Sebastian Lienemann, Martin Ehrbar

Wiley-Blackwell2013

Diagnostic microchip to assay 3D colony-growth potential of captured circulating tumor cells

Matthias Lütolf, Stefan Kobel, Samy Gobaa

Microfluidic technology has been successfully applied to isolate very rare tumor-derived epithelial cells (circulating tumor cells, CTCs) from blood with relatively high yield and purity, opening up exciting prospects for early detection of cancer. However ...

2012

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