Publications de Matthias Lütolf

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Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they are ra ...

The invention concerns a device (1) for aggregating cells comprising at least one cavity (2), wherein said cavity comprises a plurality of microwells (3) for receiving at least one cell, wherein each said well comprises a vertical sidewall and a curved bot ...

Synthetic hydrogels have been molecularly engineered to mimic the invasive characteristics of native provisional extracellular matrixes: a combination of integrin-binding sites and substrates for matrix metalloproteinases (MMP) was required to render the networks degradable and invasive by cells via cell-secreted MMPs. Degrdn. of gels was engineered starting from a characterization of the degrdn. kinetics (kcat and Km) of synthetic MMP substrates in the sol. form and after crosslinking into a 3D hydrogel ne

A new chem. crosslinking scheme was utilized for the formation of degradable poly(ethylene glycol) hydrogels suitable for the delivery of protein drugs. An aq. soln. contg. a PEG-multiacrylate and solid particles of albumin was mixed with an aq. soln. contg. a PEG-dithiol, rapidly producing a cross-linked hydrogel through a Michael-type addn. reaction. For some formulations, it was obsd. that about 65% of the incorporated protein was released with zero-order kinetics over a period of about 4 days. By changi

Many crucial cellular processes in our tissues are governed by complex, spatio-temporally regulated interactions between cells and their extracellular matrix (ECM). These interactions can be studied using well-established 3D in vitro model systems such as ...

Synthetic bioactive hydrogels have been widely recognized as key elements of emerging strategies to engineer tissues. However, the current shortage of highly specific and biocompatible methods to form and functionalize these materials hampers their wide pharmaceutical and medical use. In particular, enzymatic reactions are underexplored for the synthesis of bioactive hydrogels. Here, we present an approach by which phosphopantetheinyl transferase (PPTase), a small (16.2 kDa) enzyme that plays a key role in

Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...

Conventional in situ hydrogel micropatterning techniques work successfully for relatively stiff hydrogels, but they often result in locally damaged surfaces upon demolding in the case of soft and fragile polymer networks formed at low precursor concentrati ...

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...

The fabrication of microfluidic devices is often still a time-consuming and costly process. Here we introduce a very simple and cheap microfabrication process based on "razor writing", also termed xurography, for the ultra-rapid prototyping of microfluidic ...

The development of new drugs is currently a long and costly process in large part due to the failure of promising drug candidates identified in initial in vitro screens to perform as intended in vivo. New approaches to drug screening are being developed wh ...

Organotypic models of patient-specific tumours are revolutionizing our understanding of cancer heterogeneity and its implications for personalized medicine. These advancements are, in part, attributed to the ability of organoid models to stably preserve ge ...

Three-dimensional (3D) bioprinting strategies use computer-aided processes to enable automated simultaneous spatial patterning of cells and/or biomaterials. These technologies are suitable for a broad range of biomedical applications owing to their capabil ...

Mammalian cell behavior is strongly influenced by physical and chemical cues originating from the extracellular matrix (ECM). In vivo, ECM signals are displayed in a spatiotemporally complex fashion, often composed as gradients and in concentration profile ...

The neurosphere assay is the standard retrospective assay to test the self-renewal capability and multipotency of neural stem cells (NSC) in vitro. However, it has recently become clear that not all neurospheres are derived from a NSC and that on conventio ...

Biomaterials are increasingly being developed as in vitro microenvironments mimicking in vivo stem cell niches. However, current macroscale methodologies to produce these niche models fail to recapitulate the spatial and temporal characteristics of the com ...

Organoids form through self-organization processes in which initially homogeneous populations of stem cells spontaneously break symmetry and undergo in-vivo-like pattern formation and morphogenesis, though the processes controlling this are poorly characte ...

Adult stem cells (SCs) are essential for tissue maintenance and regeneration yet are susceptible to senescence during aging. We demonstrate the importance of the amount of the oxidized form of cellular nicotinamide adenine dinucleotide (NAD(+)) and its imp ...

Matthias Lütolf

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A functional genetic toolbox for human tissue-derived organoids

Devices for high-throughput aggregation and manipulation of mammalian cells

Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: Engineering cell-invasion characteristics

Protein delivery from materials formed by self-selective conjugate addition reactions

Integration column: Artificial ECM: expanding the cell biology toolbox in 3D

Phosphopantetheinyl transferase-catalyzed formation of bioactive hydrogels for tissue engineering

Organoids as tools for fundamental discovery and translation-A Keystone Symposia report

Micropatterning of Hydrogels by Soft Embossing

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells

Dynamic Hydrogel Design For Spatiotemporal Control Of Morphogenesis

Ultra-rapid prototyping of flexible, multi-layered microfluidic devices via razor writing

Drug discovery through stem cell-based organoid models

Next-generation cancer organoids

3D extrusion bioprinting

Microscale patterning of hydrogel stiffness through light-triggered uncaging of thiols

Enhancing the Reliability and Throughput of Neurosphere Culture on Hydrogel Microwell Arrays

Biomaterials meet microfluidics: building the next generation of artificial niches

Engineering Stem Cell Self-organization to Build Better Organoids

NAD+ repletion improves mitochondrial and stem cell function and enhances life span in mice