Publication

Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening

Gerardo Turcatti
2015
Book Chapters

Abstract

Cellular assays using label-free Digital Holographic Microscopy (DHM) have been previously validated for cell viability assays in a drug screening context. Our automated DHM system allows performing fast and cost-effective screening assays for a wide range of applications for monitoring cell morphological changes and cell movements upon interaction with interfering compounds. In addition to these classic phenotypic assays, it has been demonstrated that target-based cellular assays can also be addressed by DHM for therapeutically relevant chloride channel receptors. Our DH-imaging (DHI) technology, potentially scalable for screening by imaging approaches in a high-throughput manner can also deliver highly informative data through long term experiments. Three examples of phenotypic screens are detailed in the present chapter: a label-free profiling approach, a cell proliferation assay, and methods for monitoring the activity of the GABAA chloride channel receptor.

Official source

https://infoscience.epfl.ch/entities/publication/dc4e6bac-f56e-44a0-9d27-a42f2fb890c7

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Gerardo Turcatti
2015
Book Chapters

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/dc4e6bac-f56e-44a0-9d27-a42f2fb890c7

About this result

Related concepts (32)

High-throughput screening

High-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology, materials science and chemistry. Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to quickly conduct millions of chemical, genetic, or pharmacological tests. Through this process one can quickly recognize active compounds, antibodies, or genes that modulate a particular biomolecular pathway.

High-content screening

High-content screening (HCS), also known as high-content analysis (HCA) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell in a desired manner. Hence high content screening is a type of phenotypic screen conducted in cells involving the analysis of whole cells or components of cells with simultaneous readout of several parameters.

Chemoproteomics

Chemoproteomics (also known as chemical proteomics) entails a broad array of techniques used to identify and interrogate protein-small molecule interactions. Chemoproteomics complements phenotypic drug discovery, a paradigm that aims to discover lead compounds on the basis of alleviating a disease phenotype, as opposed to target-based drug discovery (reverse pharmacology), in which lead compounds are designed to interact with predetermined disease-driving biological targets.

Related publications (6)

Digital Holographic Imaging for Label-Free Phenotypic Profiling, Cytotoxicity, and Chloride Channels Target Screening

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