Publication

Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System

Gerardo Turcatti, Marc Chambon
2025

Abstract

Stress response pathways rely on coordinated gene expression regulation. In the endoplasmic reticulum, the unfolded protein response plays an important role in maintaining the homeostasis of protein folding and processing. A chemigenetic reporter assay is developed that allows monitoring of BiP/GRP78 expression, a key regulator of the unfolded protein response. The system is based on the coexpression of the self‐labeling protein HaloTag via an internal ribosome entry site. HaloTag allows for flexibility in labeling color as well as labeling timepoint. A two‐color timestamping strategy is designed that provides improved readout sensitivity. The reporter system can be used in live‐cell imaging as well as flow cytometry. It is applied in a high‐content screening experiment in which a new promising activator of BiP/GRP78 expression was identified.

Official source

https://infoscience.epfl.ch/entities/publication/ee542f45-907d-4220-82e8-1ee1f779b815

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Gerardo Turcatti, Marc Chambon
2025

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/ee542f45-907d-4220-82e8-1ee1f779b815

About this result

Related concepts (32)

Gene expression

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA.

Chaperone (protein)

In molecular biology, molecular chaperones are proteins that assist the conformational folding or unfolding of large proteins or macromolecular protein complexes. There are a number of classes of molecular chaperones, all of which function to assist large proteins in proper protein folding during or after synthesis, and after partial denaturation. Chaperones are also involved in the translocation of proteins for proteolysis. The first molecular chaperones discovered were a type of assembly chaperones which assist in the assembly of nucleosomes from folded histones and DNA.

Gene expression profiling

In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell. Several transcriptomics technologies can be used to generate the necessary data to analyse.

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Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System

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