Publication

Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

Kai Johnsson, Hirohito Haruki, Monica Rengifo Gonzalez
2012
Journal Articles

Abstract

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.

Official source

https://infoscience.epfl.ch/entities/publication/c0f236e2-0622-4c17-ac99-ddb322a2bd36

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Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

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