Publication

Towards the fluorescence resonance energy transfer (FRET) scanning near-field optical microscopy: Investigation of nanolocal FRET processes and FRET probe microscope

Majed Chergui, Giovanni Dietler
2000
Journal Articles

Abstract

The fluorescence resonance energy-transfer (FRET) process was studied between donor dye mols. deposited on the sample surface and acceptor dye mols. deposited on the tips of scanning near-field and at. force microscopes. The FRET process was obsd. only when the tip acquired contact with the sample and took place in regions of sizes of only a few tens of nanometers with only a few thousands (or even hundreds) of mols. involved. The dependence of the FRET intensity on the tip-sample acting force is recorded and interpreted. In relation to the obtained results, the construction of a previously proposed 1-atom FRET SNOM is described. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/833c5fab-33e4-479d-9021-cbd6d3b1e3de

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Majed Chergui, Giovanni Dietler
2000
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/833c5fab-33e4-479d-9021-cbd6d3b1e3de

About this result

Related concepts (24)

Scanning probe microscopy

Scanning probe microscopy (SPM) is a branch of microscopy that forms images of surfaces using a physical probe that scans the specimen. SPM was founded in 1981, with the invention of the scanning tunneling microscope, an instrument for imaging surfaces at the atomic level. The first successful scanning tunneling microscope experiment was done by Gerd Binnig and Heinrich Rohrer. The key to their success was using a feedback loop to regulate gap distance between the sample and the probe.

Förster resonance energy transfer

Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole–dipole coupling. The efficiency of this energy transfer is inversely proportional to the sixth power of the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance.

Atomic force microscopy

Atomic force microscopy (AFM) or scanning force microscopy (SFM) is a very-high-resolution type of scanning probe microscopy (SPM), with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit. Atomic force microscopy (AFM) is a type of scanning probe microscopy (SPM), with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit.

Related publications (6)

Towards the fluorescence resonance energy transfer (FRET) scanning near-field optical microscopy: Investigation of nanolocal FRET processes and FRET probe microscope

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