A method for introducing a nucleic acid into a eukaryotic cell

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Frozen, the movie, released in 2013, made a big impression on many people: some children were no doubt amazed by the talking snowman, others by the film’s music, the designers were probably more interesed in the reproduction quality of the snow modelled, by the Material Point Method (MPM). Many paperspraise the effectiveness of this method for simulating complex phenomena. However, very few studies have been carried out to verify the method on very simple academic problems; that is precisely what we will tr

In this paper, we present a multilevel Monte Carlo (MLMC) version of the Stochastic Gradient (SG) method for optimization under uncertainty, in order to tackle Optimal Control Problems (OCP) where the constraints are described in the form of PDEs with random parameters. The deterministic control acts as a distributed forcing term in the random PDE and the objective function is an expected quadratic loss. We use a Stochastic Gradient approach to compute the optimal control, where the steepest descent directi

In this paper, we consider unilateral contact problem without friction between a rigid body_and deformable one in the framework of isogeometric analysis. We present the theoretical_analysis of the mixed problem using an active-set strategy and for a primal space of NURBS_of degree

p

and

p-2

for a dual space of B-Spline. A inf--sup stability is proved to ensure_a good property of the method. An optimal a priori error estimate is demonstrated without_assumption on the unknown contact set. Several numerica

Transient Stability Assessment (TSA) is the process in which the stability of a system is characterized qualitatively or quantitatively. The TSA algorithm presented in this paper is derived from the well-established Single Machine Equivalent (SIME) method and can thus be categorized as a hybrid direct-temporal method. The novelty of the proposed algorithm is that it derives a Transient Stability Index (TSI) with a single TimeDomain (TD) simulation for both stable and unstable cases. The resulting TSI is uni

Cellular signalling is classically investigated by measuring optical or electrical properties of single or populations of living cells. Here we show how cell-derived vesicles can be used for anlaysing transmembrane signalling. The vesicles are derived from live mammalian cells by using either chemicals, or by optical tweezers and they comprise parts of the plasma membrane and cytosol of the mother cell. We measured in vesicles derived from individual cells with single molecule sensitivity the different step

Researchers and developers typically use I-V curves (current vs. potential) as a means to demonstrate good data fitting of a model under experimental validation. Despite the popularity of this method, assessment of the results can be intuitive rather than mathematical, and usually, when such a demonstration occurs, information on the quality of the parameter estimation is not given. On the contrary, it has been shown that simple model fitting on I–V curves cannot give statistically reliable results on the v

During infection in humans, P. falciparum invades and refurbishes the red blood cells (RBCs) in order to persist and proliferate. Parasite survival depends on expression of a parasite encoded cytoadherence ligand at the surface of the infected RBC called PfEMP1 (P. falciparum erythrocyte membrane protein 1). PfEMP1 comprises a N-terminal extracellular part, which mediates binding to receptors at the host endothelium. It also contains a C-terminal part which is conserved among all PfEMP1 molecules. This acid

The paper presents a method to determine hysteresis losses in synchronous permanent magnet (M) slotless motors. The method comprises four steps: (1) experimental determination of the B-H loop, () analytical modeling of the B-H loop by applying different models, (3) analytical modeling of the hysteresis losses, and (4) determination of the hysteresis losses in a synchronous PM motor, and results verification using an experiment and an FEM software. This paper also presents an innovative and simple way to tak

We propose an extension of secant methods for nonlinear equations using a population of previous iterates. Contrarily to classical secant methods, where exact interpolation is used, we prefer a least squares approach to calibrate the linear model. We propose an explicit control of the numerical stability of the method. We show that our approach can lead to an update formula. In that case, we prove the local convergence of the corresponding undamped quasi-Newton method. Finally, computational comparisons wit

This paper proposes a new method for reconstruction of star-shaped 3D surfaces from scattered datasets, where such surfaces are considered as signals living in the space of square integrable functions on the unit sphere. We first propose a generalization of the Fourier transform on the sphere. A practical reconstruction method is then presented, which interpolates a spherical signal on an equiangular grid, from non-uniformly sampled dataset representing a 3D point cloud. The experiments show that the propos

We analyze iterative coding for the frame-synchronous binary-input discrete-time Gaussian multiple access channel. Using the method of Density Evolution, we optimize degree distributions to exhibit code ensembles whose iterative decoding thresholds are only few tenths of dB away from the Shannon limit.

In this paper, a method for sensorless position estimation of linear transducers at standstill is suggested. The method makes use of current pulses, which is a common sensorless starting technique for 3-phase synchronous motors. Most transducers possess however only one phase. In order to find a unique solution, the proposed method differs in some important aspects from the methods used in 3-phase systems. A general discussion of different inductance phenomena is presented. A combination of these phenomena

A nonlinear method has been developed to separate arterial pressure and flow waves into their forward and backward running components. It takes into account nonlinearities arising from the area-pressure relationship as well as convective acceleration. The method is based on the treatment of the Riemann invariants and requires measurements of pressure, flow and diameter at one arterial location. The method has been successfully tested by means of a simulation experiment in which the forward and backward wave

A method for introducing a nucleic acid into a eukaryotic cell

Chat with Graph Search

MATERIAL POINT METHOD A numerical evaluation for elastic problems

MATHICSE Technical Report : A Multilevel Stochastic Gradient method for PDE-constrained Optimal Control Problems with uncertain parameters

MATHICSE Technical Report : A priori error for unilateral contact problems with Lagrange multipliers and IsoGeometric Analyis

A transient stability assessment method using post-fault trajectories

Cell-derived plasma membrane vesicles as minimal cells for analyzing transmembrane signaling

A Parameter Estimation Method for Fuel Cell Diagnostics

Proteins interacting with PfEMP1 in P. falciparum infected erythrocyte

Modeling of Hysteresis Losses Applied to Slotless Permanent Magnet Motors

A multi-iterate method to solve systems of nonlinear equations

FST-based Reconstruction of 3D-models from Non-Uniformly Sampled Datasets on the Sphere

Achieving General Points in the 2-User Gaussian MAC Without Time-Sharing or Rate-Splitting by Means of Iterative Coding

Sensorless position estimation of linear voice-coil transducers

Forward and backward waves in the arterial system: nonlinear separation using Riemann invariants