Converting disulfide bridges in native peptides to stable methylene thioacetals

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Tris-(2-carboxyethyl)phosphine (TCEP) linked to agarose beads is widely used for reducing disulfide bridges in proteins and peptides. The immobilization of TCEP on beads allows efficient removal after reduction to prevent its reaction with alkylating reage ...

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Thiol groups are suitable handles for site-selectively modifying, immobilizing or cyclizing individual peptides or entire peptide libraries. A limiting step in producing the thiol-functionalized peptides is the chromatographic purification, which is partic ...

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The disulfide bonds that form between two cysteine residues are important in defining and rigidifying the structures of proteins and peptides. In polypeptides containing multiple cysteine residues, disulfide isomerization can lead to multiple products with ...

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Converting disulfide bridges in native peptides to stable methylene thioacetals

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Engineering of stable and functional metalloproteins using evolutionary computation, deep learning and simulation

Benchmarking machine-readable vectors of chemical reactions on computed activation barriers

Methods for high-throughput synthesis and screening of peptide libraries

High-Density Immobilization of TCEP on Silica Beads for Efficient Disulfide Reduction and Thiol Alkylation in Peptides

Development of methods for the synthesis of large combinatorial libraries of macrocyclic compounds

Solid-phase peptide synthesis on disulfide-linker resin followed by reductive release affords pure thiol-functionalized peptides

Cyclative Release Strategy to Obtain Pure Cyclic Peptides Directly from the Solid Phase

Methods for the generation of large combinatorial macrocycle libraries

A Rosetta-based protein design protocol converging to natural sequences

Repair or Degrade: the Thermodynamic Dilemma of Cellular Protein Quality-Control

Application of Hypervalent Iodine Reagents for Novel Cysteine Labeling

Synthesis of DNA‐encoded disulfide‐ and thioether‐cyclized peptides

Can Mutational Analysis Be Used To Assist Structure Determination of Peptides?

Molecular Imaging of Biopolymers Enabled by Electrospray-Ion Beam Deposition

Noncovalent Protein and Peptide Functionalization of Single-Walled Carbon Nanotubes for Biodelivery and Optical Sensing Applications

Development of Stapling Methods by Selective Side Chain Modifications of Bioactive Peptides

Middle-down approaches for mass spectrometry-based protein identification and characterization

Dithiol amino acids can structurally shape and enhance the ligand-binding properties of polypeptides

Spectroscopic studies of peptide fragments produced by collision-induced dissociation

Development of bicyclic peptide Her2 binders and phage selection of alpha-helical peptide ligands