Protein-functionalized polymer brushes

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Proteins are foundational biomolecules of life playing a crucial role in a myriad of biological processes. Their function often requires interplay with other biomolecules, including proteins themselves. Protein-protein interactions (PPIs) are essential for ...

Aortic diseases are characterized by dire prognosis and inadequate diagnosis, owing to their insidious yet lethal nature. Aortopathies, namely aortic aneurysms and dissections, along with certain congenital diseases, might necessitate surgical replacement ...

The goal of my PhD was the development of a new method for unselective surface labeling, not substrate-specific, that would give access to direct visualization of any surface interactions. In order to achieve this challenging goal, we wanted to develop a g ...

The development of innovative bioanalytical technologies that enable the discovery of new therapeutics or the creation of systems for rapid, accurate and parallel analyses has gained an extraordinary interest in the biomedical sector in the last 10-15 year ...

This manuscript reports on the post-polymerization modification of poly(glycidyl methacrylate) (PGMA) and PGMA-co-poly(2-(diethylamino)ethyl methacrylate) (PGMA(x)-co-PDEAEMA(y)) (co)polymer brushes prepared via surface-initiated atom transfer radical poly ...

The mis-regulation of gene transcription in the living cell can be the cause of complex human diseases like cancer. One challenge of the post-genomic era of biomedical research is the development of in vitro techniques for the rapid and cost-effective anal ...

Surface-initiated controlled radical polymerization is a powerful strategy to tailor the chemical and physical surface properties of materials. This article highlights recent work from the author's laboratory in which surface-initiated atom transfer radica ...

Telomeres are specialized nucleoprotein structures present at the ends of linear chromosome. The telomeric DNA part is comprised of 5-15 kilo base pairs of double stranded TTAGGG repeats and it contains at the 3' end a single-stranded G-rich overhang of 50 ...

The term polymer brush refers to a well defined arrangement of polymer chains, which are tethered with one end to an interface and usually the surface of a solid substrate. Due to steric repulsion the polymer chains furthermore adopt a defined, stretched c ...

Here we discuss, how by applying chemical concepts to biological problems, methods have been developed to map spatiotemporal regulation of proteins and small-molecule modulation of proteome signaling responses. We outline why chemical-biology platforms are ...

With the human genome and many other eukaryotic and procaryotic genomes sequenced, the interest in life sciences is shifting towards the examination of the function, modification and regulation of the encoded proteins. Proteins are involved in virtually al ...

Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...

The research described within this thesis is primarily motivated by two fields of science with reversed, yet complementary, approaches to addressing the same essential objective: manipulating and understanding the complex program of life. Whereas synthetic ...

Protein microarrays are an attractive approach for the high-throughput analysis of protein function, but their impact on proteomics has been limited by the technical difficulties associated with their generation. Here we demonstrate that fusion proteins of ...

A new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subse ...

The surface chemistry at the DNA recognition and binding interface plays an important role in determining the performance of DNA microarrays and DNA biosensors. Surface-initiated controlled radical polymerization (SI-CRP) reactions represent a powerful too ...

Gene product 5 (gp5) of bacteriophage T4 is a spike-shaped protein that functions to disrupt the membrane of the target cell during phage infection. Its C-terminal domain is a long and slender -helix that is formed by three polypeptide chains wrapped aroun ...

DNA biosensing requires high oligonudeotide binding capacity interface chemistries that can be tuned to maximize probe presentation as well as hybridization efficiency. This contribution investigates the feasibility of aldehyde-functionalized poly(2-hydrox ...

Antibody microarrays are powerful and high-throughput tools for screening and identifying tumor markers from small sample volumes of only a few microliters. Optimization of surface chemistry and spotting conditions are crucial parameters to enhance antibod ...

While antibodies currently play a dominant role as affinity reagents in biological research and for diagnostics, a broad range of recombinant proteins are emerging as promising alternative affinity reagents in detection assays and quantification. DNA-media ...

Protein-functionalized polymer brushes

Chat with Graph Search

Engineering novel protein interactions with therapeutic potential using deep learning-guided surface design

Compliance-matching vascular grafts with personalization potential

Azide-Containing Hypervalent Iodine Reagents for Biomolecules Functionalization

Polymer Brushes as Substrates for Microarray Applications

Room Temperature, Aqueous Post-Polymerization Modification of Glycidyl Methacrylate-Containing Polymer Brushes Prepared via Surface-Initiated Atom Transfer Radical Polymerization

Protein-binding microarray technology and ChIP-sequencing for the identification of novel targets of the human tumor suppressor AP2[alpha]

Biofunctional and biomimetic polymer brushes prepared via surface-initiated atom transfer radical polymerization

RADX: A novel single-stranded DNA-binding protein regulating telomere recombination

Functional polymer brushes via surface-initiated controlled radical polymerizations

Chemical Biology Gateways to Mapping Location, Association, and Pathway Responsivity

Chemical approaches to microarrays and multifunctional protein labeling

Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection

Integrating Gene Synthesis and MITOMI for Rapid Protein Engineering

Protein function microarrays based on self-immobilizing and self-labeling fusion proteins

Protein-Functionalized Polymer Brushes

Surface-initiated controlled radical polymerization enhanced DNA biosensing

Structure and Biophysical Properties of a Triple-Stranded Beta-Helix Comprising the Central Spike of Bacteriophage T4

Oligonucleotide Immobilization and Hybridization on Aldehyde-Functionalized Poly(2-hydroxyethyl methacrylate) Brushes

Characterization of Three Amino-Functionalized Surfaces and Evaluation of Antibody Immobilization for the Multiplex Detection of Tumor Markers Involved in Colorectal Cancer

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation