Protein-functionalized polymer brushes

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Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

Next generation microfluidic platforms for high-throughput protein biochemistry

Sebastian Maerkl

DNA technologies such as cloning, DNA microarrays, and next generation sequencing have transformed the life sciences. Protein technologies on the other hand have not seen such explosive progress. This is mainly due to the inherent difficulty of working wit ...

Elsevier2011

Interactive Surfaces Based on Polymer Brushes

Dusko Paripovic

While the bulk structure of a material determines its mechanical properties, its surface plays a key role when interactions with the environment are important. Nowadays materials in bio- and nanotechnology are required to possess very complex functionaliti ...

EPFL2011

Protein Microarrays Based on Polymer Brushes Prepared via Surface-Initiated Atom Transfer Radical Polymerization

Harm-Anton Klok, Raphaël Barbey

Polymer brushes represent an interesting platform for the development of high-capacity protein binding surfaces. Whereas the protein binding properties of polymer brushes have been investigated before, this manuscript evaluates the feasibility of poly(glyc ...

American Chemical Society2010