Publications by Mor-Miri Mishkovsky

Related publications (41)

Tracking Hyperpolarized [1-¹³C] Pyruvate and [1-¹³C] L-Lactate Metabolism in the Healthy and Post-Stroke Mouse Brain

Rolf Gruetter, Bernard Lanz, Mor-Miri Mishkovsky, Jean-Noël Hyacinthe, Thanh Phong Kevin Lê, Mario Gaetano Lepore, Lara Buscemi Estefanell, Elise Marie Catherine Vinckenbosch

Tracking hyperpolarized (HP) ¹³C labeling from either [1-¹³C] pyruvate or [1-¹³C] lactate is a useful tool to assess intermediary metabolism in vivo, which has already been translated from preclinical to clinical research. HP [1-¹³C] pyruvate and [1-¹³C] l ...

Wiley2025

A hybrid setup for rodent hyperpolarized metabolic imaging using a clinical magnetic resonance scanner

Mor-Miri Mishkovsky

Metabolic magnetic resonance spectroscopic imaging using hyperpolarized contrast agents offers a non-invasive approach to monitoring real-time in vivo energy metabolism. The technique involves hyperpolarizing a contrast agent in a polarizer, administering ...

2025

Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo

Arnaud Comment, Bernard Lanz, Josefina Adriana Maria Bastiaansen, Tian Cheng, Mor-Miri Mishkovsky, Hikari Ananda Infinity Yoshihara, Yuhei Takado, Sylvain Eric Lengacher

The dynamics of L-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C-13 magnetic resonance spectro ...

2018

Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging

Arnaud Comment, Tian Cheng, Mor-Miri Mishkovsky, Najat Salameh, Christophe Roussel, Andrea Capozzi, Yuhei Takado, Jean-Noël Hyacinthe, Tim Rolf Eichhorn

Hyperpolarized substrates prepared via dissolution dynamic nuclear polarization have been proposed as magnetic resonance imaging (MRI) agents for cancer or cardiac failure diagnosis and therapy monitoring through the detection of metabolic impairments in v ...

National Academy of Sciences2013

In vivo enzymatic activity of acetylCoA synthetase in skeletal muscle revealed by (13)C turnover from hyperpolarized [1-(13)C]acetate to [1-(13)C]acetylcarnitine

João Miguel das Neves Duarte, Rolf Gruetter, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Tian Cheng, Mor-Miri Mishkovsky

BACKGROUND: Acetate metabolism in skeletal muscle is regulated by acetylCoA synthetase (ACS). The main function of ACS is to provide cells with acetylCoA, a key molecule for numerous metabolic pathways including fatty acid and cholesterol synthesis and the ...

Elsevier Science Bv2013

Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments

Mor-Miri Mishkovsky

Three-dimensional nuclear magnetic resonance (3D NMR) provides one of the foremost analytical tools available for the elucidation of biomolecular structure, function and dynamics. Executing a 3D NMR experiment generally involves scanning a series of time-d ...

2007

Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized C-13 magnetic resonance

Rolf Gruetter, Arnaud Comment, Mor-Miri Mishkovsky

The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, C-13 magnetic resonance spectroscopy (MRS) allows following the fate of C-13-enriched substrates th ...

Springer Nature2017

Evaluating the potential of hyperpolarised [1-13C] L-lactate as a neuroprotectant metabolic biosensor for stroke

Mor-Miri Mishkovsky, Jean-Noël Hyacinthe, Lara Buscemi Estefanell, Thanh Phong Kevin Lê, Mario Gaetano Lepore

Cerebral metabolism, which can be monitored by magnetic resonance spectroscopy (MRS), changes rapidly after brain ischaemic injury. Hyperpolarisation techniques boost (13)C MRS sensitivity by several orders of magnitude, thereby enabling in vivo monitoring ...

Nature Research2020

Hyperpolarized MRS: New tool to study real-time brain function and metabolism

Arnaud Comment, Mor-Miri Mishkovsky

The advent of dissolution dynamic nuclear polarization (DNP) led to the emergence of a new kind of magnetic resonance (MR) measurements providing the opportunity to probe metabolism in vivo in real time. It has been shown that, following the injection of h ...

Elsevier2017

Measuring Glycolytic Activity with Hyperpolarized [2H7, U-13C6] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions

Rolf Gruetter, Bernard Lanz, Mor-Miri Mishkovsky, Andrea Capozzi, Yves Pilloud, Emmanuelle Ines Flatt

Glucose is the primary fuel for the brain; its metabolism is linked with cerebral function. Different magnetic resonance spectroscopy (MRS) techniques are available to assess glucose metabolism, providing complementary information. Our first aim was to inv ...

2021

In vivo detection of brain Krebs cycle intermediate by hyperpolarized magnetic resonance

Rolf Gruetter, Arnaud Comment, Mor-Miri Mishkovsky

The Krebs (or tricarboxylic acid (TCA)) cycle has a central role in the regulation of brain energy regulation and metabolism, yet brain TCA cycle intermediates have never been directly detected in vivo. This study reports the first direct in vivo observati ...

Nature Publishing Group2012

Hyperpolarized C-13-glucose magnetic resonance highlights reduced aerobic glycolysis in vivo in infiltrative glioblastoma

Rolf Gruetter, Arnaud Comment, Cristina Ramona Cudalbu Moldovan, Bernard Lanz, Mor-Miri Mishkovsky, Jocelyne Bloch

Glioblastoma (GBM) is the most aggressive brain tumor type in adults. GBM is heterogeneous, with a compact core lesion surrounded by an invasive tumor front. This front is highly relevant for tumor recurrence but is generally non-detectable using standard ...

Nature Research2021

Producing Radical-Free Hyperpolarized Perfusion Agents for In Vivo Magnetic Resonance Using Spin-Labeled Thermoresponsive Hydrogel

Arnaud Comment, Tian Cheng, Mor-Miri Mishkovsky

Dissolution dynamic nuclear polarization (DNP) provides a way to tremendously improve the sensitivity of nuclear magnetic resonance experiments. Once the spins are hyperpolarized by dissolution DNP, the radicals used as polarizing agents become undesirable ...

Wiley-Blackwell2016

Hyperpolarized acetate as a metabolic tracer for skeletal and cardiac muscle energetics

Rolf Gruetter, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Tian Cheng, Mor-Miri Mishkovsky

2011

Localized in vivo hyperpolarization transfer sequences

Rolf Gruetter, Arnaud Comment, Tian Cheng, Mor-Miri Mishkovsky

In vivo localized and fully adiabatic homonuclear and heteronuclear polarization transfer experiments were designed and performed in the rat brain at 9.4 T after infusion of hyperpolarized sodium [1,2-(13) C(2) ] and sodium [1-(13) C] acetate. The method p ...

Wiley-Blackwell2012

Scavenging Free Radicals To Preserve Enhancement and Extend Relaxation Times in NMR using Dynamic Nuclear Polarization

Rolf Gruetter, Geoffrey Bodenhausen, Arnaud Comment, Sandrine Gerber, Mor-Miri Mishkovsky, Sami Jannin, Pascal Miéville, Riddhiman Sarkar, Paul Romeo Vasos, Puneet Ahuja

Vitamin C for longer lifetimes: N-oxide radicals that are widely used for dynamic nuclear polarization can be reduced by scavengers such as sodium ascorbate (vitamin C) during the dissolution process, thus diminishing losses of polarization during the tran ...

Wiley-Blackwell2010

Automated transfer and injection of hyperpolarized molecules with polarization measurement prior to in vivo NMR

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Tian Cheng, Mor-Miri Mishkovsky

Hyperpolarized magnetic resonance via dissolution dynamic nuclear polarization necessitates the transfer of the hyperpolarized molecules from the polarizer to the imager prior to in vivo measurements. This process leads to unavoidable losses in nuclear pol ...

2013

Principles and Progress in Ultrafast Multidimensional Nuclear Magnetic Resonance

Mor-Miri Mishkovsky

Multidimensional acquisitions play a central role in the progress and applications of nuclear magnetic resonance (NMR) spectroscopy. Such experiments have been collected traditionally as an array of one-dimensional scans, with suitably incremented delay pa ...

2009

Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke

Mor-Miri Mishkovsky, Jean-Noël Hyacinthe, Thanh Phong Kevin Lê, Mario Gaetano Lepore, Lara Buscemi Estefanell

Hyperpolarization of 13C by dissolution dynamic nuclear polarization (dDNP) boosts the sensitivity of magnetic resonance spectroscopy (MRS), making possible the monitoring in vivo and in real time of the biochemical reactions of exogenously infused 13C-lab ...

2023

Hyperpolarized 6Li as a probe for hemoglobin oxygenation level

Rolf Gruetter, Arnaud Comment, Mor-Miri Mishkovsky, Ruud van Heeswijk, Riccardo Balzan

Hyperpolarization by dissolution dynamic nuclear polarization (DNP) is a versatile technique to dramatically enhance the nuclear magnetic resonance (NMR) signal intensity of insensitive long-T1 nuclear spins such as 6 Li. The 6 Li longitudinal relaxation o ...

Wiley-Blackwell2016