Localized in vivo hyperpolarization transfer sequences

In vivo localized and fully adiabatic homonuclear and heteronuclear polarization transfer experiments were designed and performed in the rat brain at 9.4 T after infusion of hyperpolarized sodium [1,2-(13) C(2) ] and sodium [1-(13) C] acetate. The method presented herein leads to highly enhanced in vivo detection of short-T(1) (13) C as well as attached protons. This indirect detection scheme allows for probing additional molecular sites in hyperpolarized substrates and their metabolites and can thus lead to improved spectral resolution such as in the case of (13) C-acetate metabolism.