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Giovanni Longo

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Related publications (30)

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A Rapid Unraveling of the Activity and Antibiotic Susceptibility of Mycobacteria

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Leonardo Venturelli

The development of antibiotic-resistant bacteria is a worldwide health-related emergency that calls for new tools to study the bacterial metabolism and to obtain fast diagnoses. Indeed, the conventional analysis time scale is too long and affects our abili ...

AMER SOC MICROBIOLOGY2019

Graphitic carbon in a nanostructured titanium oxycarbide thin film to improve implant osseointegration

Giovanni Longo

A nanostructured coating layer on titanium implants, able to improve their integration into bones and to protect against the harsh conditions of body fluids, was obtained by Ion Plating Plasma Assisted, a method suitable for industrial applications. A tita ...

Elsevier Science Bv2015

Identification of Oxidative Stress in Red Blood Cells with Nanoscale Chemical Resolution by Infrared Nanospectroscopy

Giovanni Dietler, Giovanni Longo, Francesco Simone Ruggeri

During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include devia ...

2018

Amyloid Single Cell Cytotoxicity Assays by Nanomotion Detection

Giovanni Dietler, Hilal Lashuel, Sandor Kasas, Giovanni Longo, Francesco Simone Ruggeri, Anne-Laure Mahul Mellier

Cells are extremely complex systems able to modify actively their metabolism and behaviour in response to environmental conditions and stimuli, such as pathogenic agents or drugs. The comprehension of these responses is central to understand the molecular ...

Springer Nature2017

Combination of fluorescence microscopy and nanomotion detection to characterize bacteria

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Justin Terence Notz Pekkanen

Antibiotic-resistant pathogens are a major health concern in everyday clinical practice. Because their detection by conventional microbial techniques requires minimally 24h, some of us have recently introduced a nanomechanical sensor, which can reveal moti ...

Wiley-Blackwell2013

Nanomechanical sensor applied to blood culture pellets: a fast approach to determine the antibiotic susceptibility against agents of bloodstream infections

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Petar Stupar

Objectives: The management of bloodstream infection, a life-threatening disease, largely relies on early detection of infecting microorganisms and accurate determination of their antibiotic susceptibility to reduce both mortality and morbidity. Recently we ...

2017

Effects of antibacterial agents and drugs monitored by atomic force microscopy

Sandor Kasas, Giovanni Longo

Originally invented for topographic imaging, atomic force microscopy (AFM) has evolved into a multifunctional biological toolkit, enabling to measure structural and functional details of cells and molecules. Its versatility and the large scope of informati ...

Wiley-Blackwell2014

Investigation of resins suitable for the preparation of biological sample for 3-D electron microscopy

Giovanni Longo

In the last two decades, the third-dimension has become a focus of attention in electron microscopy to better understand the interactions within subcellular compartments. Initially, transmission electron tomography (TEM tomography) was introduced to image ...

Elsevier2015

Detecting nanoscale vibrations as signature of life

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Francesco Simone Ruggeri, Petar Stupar

The existence of life in extreme conditions, in particular in extraterrestrial environments, is certainly one of the most intriguing scientific questions of our time. In this report, we demonstrate the use of an innovative nanoscale motion sensor in life-s ...

National Academy of Sciences2015

Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

Giovanni Dietler, Sandor Kasas, Giovanni Longo, María Inés Villalba, Leonardo Venturelli

Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantile ...

MDPI2021

Real-Time Monitoring of Protein Conformational Changes Using a Nano-Mechanical Sensor

Giovanni Dietler, Paolo De Los Rios, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy, Fabrizio Benedetti, Dusan Vobornik

Proteins can switch between different conformations in response to stimuli, such as pH or temperature variations, or to the binding of ligands. Such plasticity and its kinetics can have a crucial functional role, and their characterization has taken center ...

Public Library of Science2014

A universal fluid cell for the imaging of biological specimens in the atomic force microscope

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy, Charles Roduit

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM ...

Wiley-Blackwell2013

Improving Osteoblast Response In Vitro by a Nanostructured Thin Film with Titanium Carbide and Titanium Oxides Clustered around Graphitic Carbon

Giovanni Longo

Introduction Recently, we introduced a new deposition method, based on Ion Plating Plasma Assisted technology, to coat titanium implants with a thin but hard nanostructured layer composed of titanium carbide and titanium oxides, clustered around graphitic ...

Public Library of Science2016

A perspective view on the nanomotion detection of living organisms and its features

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Petar Stupar, María Inés Villalba, Leonardo Venturelli, Anne-Céline Verenna Kohler

The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tool ...

WILEY2020

Force volume and stiffness tomography investigation on the dynamics of stiff material under bacterial membranes

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Charles Roduit

The determination of the characteristics of micro-organisms in clinical specimens is essential for the rapid diagnosis and treatment of infections. A thorough investigation of the nanoscale properties of bacteria can prove to be a fundamental tool. Indeed, ...

Wiley-Blackwell2012

Stiffness tomography exploration of living and fixed macrophages

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Charles Roduit

Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We ...

Wiley-Blackwell2012

Time-Lapse AFM Imaging of DNA Conformational Changes Induced by Daunorubicin

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy

Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. I ...

American Chemical Society2013

Euclid: Searching for pair-instability supernovae with the Deep Survey

Yi Wang, Frédéric Courbin, Georges Meylan, Giovanni Longo

Pair-instability supernovae are theorized supernovae that have not yet been observationally confirmed. They are predicted to exist in low-metallicity environments. Because overall metallicity becomes lower at higher redshifts, deep near-infrared transient ...

EDP SCIENCES S A2022

Erythrocyte's aging in microgravity highlights how environmental stimuli shape metabolism and morphology

Giovanni Dietler, Giovanni Longo

The determination of the function of cells in zero-gravity conditions is a subject of interest in many different research fields. Due to their metabolic unicity, the characterization of the behaviour of erythrocytes maintained in prolonged microgravity con ...

Nature Research2018

Nanotools and molecular techniques to rapidly identify and fight bacterial infections

Sandor Kasas, Giovanni Longo

Reducing the emergence and spread of antibiotic-resistant bacteria is one of the major healthcare issues of our century. In addition to the increased mortality, infections caused by multi-resistant bacteria drastically enhance the healthcare costs, mainly ...

Elsevier2017