Publications by Romain Wyss | EPFL Graph Search

Related publications (11)

Nanostructured Surfaces for the Detection of Single-Particles and Single-Molecules

Biomarker detection and diagnosis are important steps in the prevention and the treatment of a disease. There is an increasing need for utmost sensitive and robust sensing devices; in this framework, subwavelength (metal) pores could contribute to a concep ...

EPFL2013

A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins

Suliana Manley, Romain Wyss, Julia Gunzenhäuser

The assembly process of the human immunodeficiency virus 1 (HIV-1) is driven by the viral polyprotein Gag. Fluorescence imaging of Gag protein fusions is widely performed and has revealed important information on viral assembly. Gag fusion proteins are com ...

Public Library Science2014

Use of fourier transform infrared spectroscopy analysis of extracellular vesicles isolated from body fluids for diagnosing, prognosing and monitoring pathophysiological states and method therfor

Horst Arthur Vogel, Romain Wyss, Luigino Grasso

The method analyzes biological fluids collected from patients, and uses attenuated total reflecting (ATR) sensors and spectral analysis by Fourier transform infrared spectroscopy (FTIR) to form a spectral fingerprint of extracellular vesicles (EVs) isolate ...

2016

Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy

Horst Arthur Vogel, Niels Erik Lion, Michel Prudent, Davide Demurtas, Romain Wyss, Luigino Grasso

We report on a generic method to detect and iden- tify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identit ...

Springer Heidelberg2015

X-ray structure of the mouse serotonin 5-HT3 receptor

Horst Arthur Vogel, Henning Paul-Julius Stahlberg, Rudolf Hovius, Cédric Deluz, Ghérici Hassaïne, Romain Wyss, Luigino Grasso, Menno Berend Tol

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly un ...

Nature Publishing Group2014

DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pH

Romain Wyss

Nanopores are under development for the detection of a variety of analytes and the investigation of chemical reactions at the single molecule level. In particular, the analysis of nucleic acid molecules is under intense investigation, including the develop ...

American Chemical Society (ACS)2009

Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

Horst Arthur Vogel, Rudolf Hovius, Romain Wyss, Joachim Piguet, Luigino Grasso, Ghérici Hassaïne, Michael Werner

Cellular signaling is classically investigated by measuring optical or electrical properties of single or populations of living cells. Here we show that ligand binding to cell surface receptors and subsequent activation of signaling cascades can be monitor ...

Public Library of Science2013

Molecular and Dimensional Profiling of Highly Purified Extracellular Vesicles by Fluorescence Fluctuation Spectroscopy

Horst Arthur Vogel, Davide Demurtas, Romain Wyss, Luigino Grasso, Camille Wolf, Hans Wolfgang Grosse

Cells secrete extracellular vesicles (EVs) into their microenvironment that act as mediators of intercellulard communication under physiological conditions and in this context also actively participate in spreading various diseases. Large efforts are curre ...

Amer Chemical Soc2014

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Pierre Gönczy, Suliana Manley, Romain Wyss, Debora Keller

SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 ...

Rockefeller University Press2014

A Zeptoliter Volume Meter for Analysis of Single Protein Molecules

Olivier Martin, Horst Arthur Vogel, Christian Santschi, Cédric Deluz, Romain Wyss, Ghérici Hassaïne

American Chemical Society (ACS)2012

Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking

Horst Arthur Vogel, Romano Tobias Wyss, Romain Wyss

Amer Soc Cell Biology2014