Publications by Graham Knott | EPFL Graph Search

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Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

We present a new approach for the automated segmentation of synapses in image stacks acquired by Electron Microscopy (EM) that relies on image features specifically designed to take spatial context into account. These features are used to train a classifie ...

This protocol describes how dendrites and axons, imaged in vivo, can subsequently be analyzed in 3D using focused ion beam scanning electron microscopy (FIBSEM). The fluorescent structures are identified after chemical fixation and their position highlight ...

CNN-based volumetric methods that label individual voxels now dominate the field of biomedical segmentation. However, 3D surface representations are often required for proper analysis. They can be obtained by post-processing the labeled volumes which typic ...

Axons are ultrathin membrane cables that are specialized for the conduction of action potentials. Although their diameter is variable along their length, how their morphology is determined is unclear. Here, we demonstrate that unmyelinated axons of the mou ...

BackgroundThe meninges, formed by dura, arachnoid and pia mater, cover the central nervous system and provide important barrier functions. Located between arachnoid and pia mater, the cerebrospinal fluid (CSF)-filled subarachnoid space (SAS) features a var ...

Glucose is the primary source of energy for the brain but also an important source of building blocks for proteins, lipids, and nucleic acids. Little is known about the use of glucose for biosynthesis in tissues at the cellular level. We demonstrate that l ...

The appearance and disappearance of dendritic spines, accompanied by synapse formation and elimination may underlie the experience-dependent reorganization of cortical circuits. The exact temporal relationship between spine and synapse formation in vivo re ...

Plasticity of cortical responses in vivo involves activity-dependent changes at synapses, but the manner in which different forms of synaptic plasticity act together to create functional changes in neurons remains unknown. We found that spike timing-induce ...

The in vitro inter-and intra-cellular distribution of an isotopically labelled ruthenium(II)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution ...

Parkinson’s disease (PD) is characterized by the accumulation of misfolded and aggregated α-synuclein (α-syn) into intraneuronal inclusions named Lewy bodies (LBs). Although it is widely believed that α-syn plays a central role in the pathogenesis of PD, t ...

Thanks to their flexibility, optical techniques could be the key to explore anatomy, plasticity, and functionality of the cerebellum. As an example, an in vivo analysis of the dynamic remodeling of cerebellar axons by nonlinear microscopy can provide funda ...

Mycobacterium tuberculosis (Mtb) cultured axenically without detergent forms biofilm-like cords, a clinical identifier of virulence. In lung-on-chip (LoC) and mouse models, cords in alveolar cells contribute to suppression of innate immune signaling via nu ...

If we are ever to unravel the mysteries of brain function at its most fundamental level, we will need a precise understanding of how its component neurons connect to each other. Electron Microscopes (EM) can now provide the nanometer resolution that is nee ...

Synaptic neurotransmission is modified at cortical connections throughout life. Varying the amplitude of the postsynaptic response is one mechanism that generates flexible signaling in neural circuits. The timing of the synaptic response may also play a ro ...

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, pre ...

Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed by ...

Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonlyfound in aquatic habitats. Within such environments, the bacterium must defend itselfagainst predatory protozoan grazers. Amoebae are prominent grazers, withAcant ...

Pacinian corpuscles are among the most sensitive mechanoreceptors found in vertebrates, and they are tuned to vibrations in the highest perceptible frequency range (100 to 2000 Hz). One of their anatomical hallmarks is the onion-like cell layers surroundin ...

We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with

Graham Knott

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Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Learning Context Cues for Synapse Segmentation

Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure

Voxel2Mesh: 3D Mesh Model Generation from Volumetric Data

Membrane mechanics dictate axonal pearls-on-a-string morphology and function

Large-scale morphometry of the subarachnoid space of the optic nerve

Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose

The Relationship between PSD-95 Clustering and Spine Stability In Vivo

Locally coordinated synaptic plasticity of visual cortex neurons in vivo

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

The process of Lewy body formation, rather than simply α-synuclein fibrillization, is one of the major drivers of neurodegeneration

Multi-Modal Optical Imaging of the Cerebellum in Animals

Mechanopathology of biofilm-like Mycobacterium tuberculosis cords

Modeling Brain Circuitry over a Wide Range of Scales

Delayed and Temporally Imprecise Neurotransmission in Reorganizing Cortical Microcircuits

Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons

Is EM dead?

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

Lamellar Schwann Cells in the Pacinian Corpuscle Potentiate Vibration Perception

Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution