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Graham Knott

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Related publications (97)

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Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Romain Guiet, Claudia Battistella

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

Learning Context Cues for Synapse Segmentation

Pascal Fua, Graham Knott, Carlos Joaquin Becker, Karim Ali

We present a new approach for the automated segmentation of synapses in image stacks acquired by Electron Microscopy (EM) that relies on image features specifically designed to take spatial context into account. These features are used to train a classifie ...

Institute of Electrical and Electronics Engineers2013

Voxel2Mesh: 3D Mesh Model Generation from Volumetric Data

Pascal Fua, Graham Knott, Edoardo Remelli, Pamuditha Udaranga Wickramasinghe

CNN-based volumetric methods that label individual voxels now dominate the field of biomedical segmentation. However, 3D surface representations are often required for proper analysis. They can be obtained by post-processing the labeled volumes which typic ...

2020

Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure

Pascal Fua, Graham Knott, Anne Alison Jorstad, Bohumil Maco

This protocol describes how dendrites and axons, imaged in vivo, can subsequently be analyzed in 3D using focused ion beam scanning electron microscopy (FIBSEM). The fluorescent structures are identified after chemical fixation and their position highlight ...

Elsevier Academic Press Inc2014

Deep Active Surface Models

Pascal Fua, Graham Knott, Pamuditha Udaranga Wickramasinghe

Active Surface Models have a long history of being useful to model complex 3D surfaces. But only Active Contours have been used in conjunction with deep networks, and then only to produce the data term as well as meta-parameter maps controlling them. In th ...

2021

Membrane mechanics dictate axonal pearls-on-a-string morphology and function

Graham Knott

Axons are ultrathin membrane cables that are specialized for the conduction of action potentials. Although their diameter is variable along their length, how their morphology is determined is unclear. Here, we demonstrate that unmyelinated axons of the mou ...

2025

Large-scale morphometry of the subarachnoid space of the optic nerve

Graham Knott, Gilles Fourestey

BackgroundThe meninges, formed by dura, arachnoid and pia mater, cover the central nervous system and provide important barrier functions. Located between arachnoid and pia mater, the cerebrospinal fluid (CSF)-filled subarachnoid space (SAS) features a var ...

BMC2023

Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose

Anders Meibom, Arnaud Comment, Graham Knott, Stéphane Laurent Escrig, Yuhei Takado

Glucose is the primary source of energy for the brain but also an important source of building blocks for proteins, lipids, and nucleic acids. Little is known about the use of glucose for biosynthesis in tissues at the cellular level. We demonstrate that l ...

Elsevier2015

The Relationship between PSD-95 Clustering and Spine Stability In Vivo

Graham Knott, Bohumil Maco

The appearance and disappearance of dendritic spines, accompanied by synapse formation and elimination may underlie the experience-dependent reorganization of cortical circuits. The exact temporal relationship between spine and synapse formation in vivo re ...

Soc Neuroscience2014

Locally coordinated synaptic plasticity of visual cortex neurons in vivo

Graham Knott, Sami El-Boustani

Plasticity of cortical responses in vivo involves activity-dependent changes at synapses, but the manner in which different forms of synaptic plasticity act together to create functional changes in neurons remains unknown. We found that spike timing-induce ...

American Association for the Advancement of Science2018

Focused Ion Beam Nano-Tomography Using Different Detectors

Cécile Hébert, Graham Knott, Marco Cantoni, Pierre Burdet

Cambridge, Cambridge University Press2011

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

Paul Joseph Dyson, Anders Meibom, Kai Johnsson, Graham Knott, Stéphane Laurent Escrig, Mary-Claude Croisier-Coeytaux

The in vitro inter-and intra-cellular distribution of an isotopically labelled ruthenium(II)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution ...

Royal Society of Chemistry2015

The process of Lewy body formation, rather than simply α-synuclein fibrillization, is one of the major drivers of neurodegeneration

Laura Emmeline H Weerens, Hilal Lashuel, Graham Knott, Johannes Burtscher, Marion Leleu, Fabien Kuttler, Mary-Claude Croisier-Coeytaux, Anne-Laure Mahul Mellier, Niran Maharjan

Parkinson’s disease (PD) is characterized by the accumulation of misfolded and aggregated α-synuclein (α-syn) into intraneuronal inclusions named Lewy bodies (LBs). Although it is widely believed that α-syn plays a central role in the pathogenesis of PD, t ...

National Academy of Sciences2020

Multi-Modal Optical Imaging of the Cerebellum in Animals

Graham Knott

Thanks to their flexibility, optical techniques could be the key to explore anatomy, plasticity, and functionality of the cerebellum. As an example, an in vivo analysis of the dynamic remodeling of cerebellar axons by nonlinear microscopy can provide funda ...

Springer2016

Mechanopathology of biofilm-like Mycobacterium tuberculosis cords

Maria Gabriela Kirsch, Graham Knott, John McKinney, Neeraj Dhar, Nicolas Jean Philippe Guex, Mélanie Thérèse Marie Hannebelle, Vivek Vijay Thacker, Jessica Sordet, Kunal Sharma, Maxime Jan, Anaëlle Fabienne Dubois, Richa Mishra

Mycobacterium tuberculosis (Mtb) cultured axenically without detergent forms biofilm-like cords, a clinical identifier of virulence. In lung-on-chip (LoC) and mouse models, cords in alveolar cells contribute to suppression of innate immune signaling via nu ...

Cell Press2023

Modeling Brain Circuitry over a Wide Range of Scales

Pascal Fua, Graham Knott

If we are ever to unravel the mysteries of brain function at its most fundamental level, we will need a precise understanding of how its component neurons connect to each other. Electron Microscopes (EM) can now provide the nanometer resolution that is nee ...

Frontiers Research Foundation2015

Delayed and Temporally Imprecise Neurotransmission in Reorganizing Cortical Microcircuits

Graham Knott, Anne Alison Jorstad, Yan Liu

Synaptic neurotransmission is modified at cortical connections throughout life. Varying the amplitude of the postsynaptic response is one mechanism that generates flexible signaling in neural circuits. The timing of the synaptic response may also play a ro ...

Soc Neuroscience2015

Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons

Graham Knott, Marco Cantoni, Bohumil Maco

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, pre ...

Public Library of Science2013

Is EM dead?

Graham Knott, Christel Genoud

Since electron microscopy (EM) first appeared in the 1930s, it has held centre stage as the primary tool for the exploration of biological structure. Yet, with the recent developments of light microscopy techniques that overcome the limitations imposed by ...

Company Of Biologists Ltd2013

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

Audrey Sophie Vanhove, Graham Knott, Melanie Blokesch, Sandrine Stutzmann, Catherine Maclachlan, Natalia Carolina Drebes Dorr, Candice Stoudmann, Stéphanie Clerc, Charles Ulric V Van der Henst

Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonlyfound in aquatic habitats. Within such environments, the bacterium must defend itselfagainst predatory protozoan grazers. Amoebae are prominent grazers, withAcant ...

Nature Research2018