Claudio Dellagiacoma

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Living organisms consist of cells, the elementary components of which are proteins providing cellular structures and functionality. Nowadays, proteins of the size of one to a few tens of nanometers can be efficiently monitored by fluorescence microscopy an ...

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

We report on the characterization of long-range surface plasmon waveguide bends at telecom wavelengths (lambda=1550 nm). The structures consist of a thin Au stripe embedded in a transparent polymer film. When the polymer thickness is larger than the latera ...

The invention describes a method and a microscopy system for imaging and analysing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction o ...

The diffusion of charged proteins in liquid-filled nanometer-sized apertures with charged surfaces has been investigated with fluorescence correlation spectroscopy (FCS). Based on a two-dimensional (2D) multicomponent diffusion model, key parameters such a ...

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time usi ...

Claudio Dellagiacoma

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Single Molecule Sensing

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Experimental comparison between conventional and hybrid long-range surface plasmon waveguide bends

Cumulant microscopy

Direct Observation of Transitions between Surface-Dominated and Bulk Diffusion Regimes in Nanochannels

Comparison between SOFI and STORM

From metamaterials to plasmonics: the advancing role of metals in optics