Publication

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Theo Lasser, Kai Johnsson, Iwan Märki, Stefan Geissbühler, Claudio Dellagiacoma, Noelia Laura Bocchio, Grazvydas Lukinavicius, Sambashiva Banala
2010
Journal Articles

Abstract

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged microtubule structures with ~25 nm resolution. The described probe in combination with the versatile SNAP-tag labeling opens new possibilities for imaging biological structures at the nanoscale.

Official source

https://infoscience.epfl.ch/entities/publication/56018c5d-0f2c-4fe1-b861-c8ce72fea4ae

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Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

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